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The Role of Curcumin on Apoptosis through The RASSF1A and Bax Pathways in Breast Cancer

Indonesian Journal of Cancer Chemoprevention · Vol. 11 No. 2 (2020) · hlm. 67–74

DOI
10.14499/indonesianjcanchemoprev11iss2pp67-74
ISSN
2355-8989, 2088-0197
Terbit
2020-06-30
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Curcumin has been reported with an in vitro the cytotoxic effect on several human cancer cells. However, reports on the mode of action and detail mechanism of curcumin in breast cancer disease are limited. Hence, curcumin’s effect on the human breast cancer cell line MCF-7 and MDA-MB-468 was investigated. The MCF-7 and MDA-MB-468 breast cancer cells line were given curcumin in several doses. The anti-proliferation activity of curcumin was determined using the MTS cell viability test and caspase-3 activity was used to detect apoptosis using flowcytometry. The expression of Ras-association domain family 1 isoform A (RASSF1A) and Bax protein in cells was evaluated by ELISA analysis. Kruskal-Wallis followed by the Mann-Whitney test and the Spearman correlation tests were used to asses correlation among RASSF1A, Bax, and caspase-3. Cytotoxicity of curcumin on MCF-7 was lower than that of MDA-MB-468 (75.73 μg/mL and 380.79 μg/mL). The concentration of curcumin at 80 μg/mL induced apoptosis mainly through the intrinsic pathway by caspase-3 activation. Curcumin also showed an anti-proliferative activity as shown by the increase of RASSF1A and Bax protein. Curcumin mediates anti-proliferative and apoptotic effect through the activation of RASSF1A and Bax. Our research data adds information about the role of curcumin in epigenetic events through RASSF1A protein.Keywords: Bax, caspase-3, curcumin, MCF-7, MDA-MB-468, RASSF1A

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14 penulis · 39 tautan kepenulisan bersama · 26 lintas organisasi

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  • Yarsi University
  • University of Indonesia
  • Dharmais Cancer Hospital
  • Padjadjaran University
  • National University of Malaysia
  • University of Technology Malaysia
  • Lampung University
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Rekan penulisOrganisasiArtikel bersama
Kuslestari KuslestariYarsi University2
W.N. Wan ZurinahAfiliasi tidak diketahui1
M.Y. Yasmin AnumNational University of Malaysia1
Noor Azian MoradUniversity of Technology Malaysia1
Widayanti WidayantiLampung University1
Linda WeniYarsi University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">The Role of Curcumin on Apoptosis through The RASSF1A and Bax Pathways in Breast Cancer</dc:title>
	<dc:creator>Rahmah, Nunung Ainur</dc:creator>
	<dc:creator>Harliansyah, Harliansyah</dc:creator>
	<dc:creator>Suyatna, Fransiscus D.</dc:creator>
	<dc:creator>Kanoko, Mpu</dc:creator>
	<dc:creator>Rustamadji, Primariadewi</dc:creator>
	<dc:creator>Prihartono, Joedo</dc:creator>
	<dc:creator>Haryono, Samuel Johny</dc:creator>
	<dc:creator>Hernowo, Bethy Suryawati</dc:creator>
	<dc:description xml:lang="en-US">Curcumin has been reported with an in vitro the cytotoxic effect on several human cancer cells. However, reports on the mode of action and detail mechanism of curcumin in breast cancer disease are limited. Hence, curcumin’s effect on the human breast cancer cell line MCF-7 and MDA-MB-468 was investigated. The MCF-7 and MDA-MB-468 breast cancer cells line were given curcumin in several doses. The anti-proliferation activity of curcumin was determined using the MTS cell viability test and caspase-3 activity was used to detect apoptosis using flowcytometry. The expression of Ras-association domain family 1 isoform A (RASSF1A) and Bax protein in cells was evaluated by ELISA analysis. Kruskal-Wallis followed by the Mann-Whitney test and the Spearman correlation tests were used to asses correlation among RASSF1A, Bax, and caspase-3. Cytotoxicity of curcumin on MCF-7 was lower than that of MDA-MB-468 (75.73 μg/mL and 380.79 μg/mL). The concentration of curcumin at 80 μg/mL induced apoptosis mainly through the intrinsic pathway by caspase-3 activation. Curcumin also showed an anti-proliferative activity as shown by the increase of RASSF1A and Bax protein. Curcumin mediates anti-proliferative and apoptotic effect through the activation of RASSF1A and Bax. Our research data adds information about the role of curcumin in epigenetic events through RASSF1A protein.Keywords: Bax, caspase-3, curcumin, MCF-7, MDA-MB-468, RASSF1A</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/320</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev11iss2pp67-74</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 11, No 2 (2020); 67-74</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/320/215</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>