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Caesalpinia sappan L. Ethanolic Extract Decrease Intracellular ROS Level and Senescence of 4T1 Breast Cancer Cells

Indonesian Journal of Cancer Chemoprevention · Vol. 10 No. 1 (2019) · hlm. 16–23

DOI
10.14499/indonesianjcanchemoprev10iss1pp16-23
ISSN
2355-8989, 2088-0197
Terbit
2019-02-28
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Highly and uncontrolled cell proliferation on cancer cells may boost ROS level intracellular accumulation up significantly. Sappan heartwood (Caesalpinia sappan L.) have been known to have cytotoxicity effect toward several cancer cells. This research was conducted to develop Caesalpinia sappan L. heartwood ethanolic extract (CSE) as chemopreventive agent which can be used as cancer therapy, looked from antioxidant and anti-senescence activity toward 4T1 breast cancer cell. CSE was obtained through maceration using ethanol 70% as solvent. Cytotoxicity activity of CSE was done by using MTT assay with IC50 as parameter. This IC50 was used as basic to the next assay, ROS assay by using DCFDA staining flowcytometry to look ROS level intracellular of CSE toward 4T1 cell and senescence assay by using senescence associated β-galactosidase (SA β-gal) assay with % cell senescence as their parameter. CSE toxic to 4T1 cell that proved from IC50 value of 25 µg/mL. Single treatment of CSE on concentration 12,5; 25; and 37.5 µg/mL able to suppress ROS level intracellular. If compared with untreated cell, single treatment of CSE on concentration 6 and 12 µg/mL showed % cell senescence not significantly different. Based on this result, CSE is cytotoxic, has antioxidant and antisenescence activity, so it has great potential to developed as chemoprevention agent on cancer therapy.Keywords: Caesalpinia sappan L., 4T1 breast cancer line, Reactive Oxygen Species (ROS), anti-senescence

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Kementerian Kesehatan Republik Indonesia
  • Nara Institute of Science and Technology
  • Atma Jaya University Yogyakarta
  • Muhammadiyah University Purwokerto
  • Tanjungpura University
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Rekan penulisOrganisasiArtikel bersama
Ratna Asmah SusidartiUniversitas Gadjah Mada20
Riris Istighfari JenieUniversitas Gadjah Mada18
Muthi IkawatiUniversitas Gadjah Mada13
Rohmad Yudi UtomoUniversitas Gadjah Mada12
Dyaningtyas Dewi Pamungkas PutriUniversitas Gadjah Mada9
Faaza Aulia RahmanUniversitas Gadjah Mada5
Sari HaryantiKementerian Kesehatan Republik Indonesia5
Dhania NovitasariUniversitas Gadjah Mada5
Desty Restia RahmawatiUniversitas Gadjah Mada5
Anindyajati AnindyajatiUniversitas Gadjah Mada5
Retno MurwantiUniversitas Gadjah Mada4
Larasati LarasatiUniversitas Gadjah Mada4
Lailatul QodriaUniversitas Gadjah Mada4
Gergorius Gena MaranUniversitas Gadjah Mada4
Yurista GilangUniversitas Gadjah Mada4
Sri HandayaniIndonesian Institute of Sciences3
Ummi Maryam ZulfinUniversitas Gadjah Mada3
Erlina RivantiUniversitas Gadjah Mada3
Sendy JunediAtma Jaya University Yogyakarta3
Sofa FaridaUniversitas Gadjah Mada3
Indri KusharyantiTanjungpura University3
Nanda Resa PratamaUniversitas Gadjah Mada3
Nindya Budiana PutriUniversitas Gadjah Mada3
Herwandhani PutriUniversitas Gadjah Mada3
Masashi KawaichiNara Institute of Science and Technology2
Nunuk Aries NurulitaMuhammadiyah University Purwokerto2
Suwijiyo PramonoUniversitas Gadjah Mada2
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Aulia Nur SeptianiUniversitas Gadjah Mada2
Beni LestariUniversitas Gadjah Mada2
Asri Mega PutriUniversitas Gadjah Mada2
Hilyatul FadliyahUniversitas Gadjah Mada2
Yonika Arum LarasatiUniversitas Gadjah Mada2
Raditya Prima IstiajiUniversitas Gadjah Mada2
Ni Putu Linda LaksmianiUdayana University2
Astrid Ayu MarutiUniversitas Gadjah Mada2

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Caesalpinia sappan L. Ethanolic Extract Decrease Intracellular ROS Level and Senescence of 4T1 Breast Cancer Cells</dc:title>
	<dc:creator>Hanif, Naufa</dc:creator>
	<dc:creator>Hermawan, Adam</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:description xml:lang="en-US">Highly and uncontrolled cell proliferation on cancer cells may boost ROS level intracellular accumulation up significantly. Sappan heartwood (Caesalpinia sappan L.) have been known to have cytotoxicity effect toward several cancer cells. This research was conducted to develop Caesalpinia sappan L. heartwood ethanolic extract (CSE) as chemopreventive agent which can be used as cancer therapy, looked from antioxidant and anti-senescence activity toward 4T1 breast cancer cell. CSE was obtained through maceration using ethanol 70% as solvent. Cytotoxicity activity of CSE was done by using MTT assay with IC50 as parameter. This IC50 was used as basic to the next assay, ROS assay by using DCFDA staining flowcytometry to look ROS level intracellular of CSE toward 4T1 cell and senescence assay by using senescence associated β-galactosidase (SA β-gal) assay with % cell senescence as their parameter. CSE toxic to 4T1 cell that proved from IC50 value of 25 µg/mL. Single treatment of CSE on concentration 12,5; 25; and 37.5 µg/mL able to suppress ROS level intracellular. If compared with untreated cell, single treatment of CSE on concentration 6 and 12 µg/mL showed % cell senescence not significantly different. Based on this result, CSE is cytotoxic, has antioxidant and antisenescence activity, so it has great potential to developed as chemoprevention agent on cancer therapy.Keywords : Caesalpinia sappan L., 4T1 breast cancer line, Reactive Oxygen Species (ROS), anti-senescence</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2019-02-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/253</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev10iss1pp16-23</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 10, No 1 (2019); 16-23</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/253/177</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Naufa Hanif</dc:rights>
</oai_dc:dc>