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Antigenotoxicity Activity of Papaya (Carica papaya L.) Leaf Ethanolic Extract on Swiss Mice Induced Cyclophosphamide through Mammalian In Vivo Micronucleus Test

Indonesian Journal of Cancer Chemoprevention · Vol. 7 No. 1 (2016) · hlm. 31–37

DOI
10.14499/indonesianjcanchemoprev7iss1pp31-37
ISSN
2355-8989, 2088-0197
Terbit
2016-02-29
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Cyclophosphamide (CPA) is an effective chemotherapeutic agent, but has side effect, causing DNA damage (genotoxic). Papaya leaf (Carica papaya L.) is known has flavonoid compound, quercetin. Quercetin is known has DNA protecting effect (antigenotoxic effect) by metabolism modulation. Thus, the aim of this research is to investigate the antigenotoxic effect of ethanolic extract of papaya (Carica papaya L.) leaf (EEPL) on CPA induced mice. The antigenotoxic effect was evaluated by mammalian in vivo micronucleus test. EEPL was orally administered as single treatment at dose 1000 mg/kgBW and in combination with CPA 50 mg/kgBW at dose 250 mg/kgBW; 500 mg/kgBW; and 1000 mg/kgBW. Molecular docking using PLANTS on CYP 3A4 was performed to explore the antigenotoxic effect mechanism. The three different combination dose of EEPL with CPA significantly (P<0.05) decreased the amount of micronucleated polychromatic erytrhocyte (MNPCE)/1000 polychromatic erythrocyte (PCE) and also increased % PCE/(PCE+normochromatic erythrocyte (NCE)), compared with single dose of CPA. Nevertheless, the antigenotoxic effect wasn’t significant compared with each combination dose. The docking score result showed quercetin (-82,41) has more potent interaction to CYP 3A4 than cyclophosphamide (-70,16) and both of them has similar active site at amino acid residue Ile 369 and Thr 309. The results obtained indicated that EEPL at dose 250 mg/KgBB is the optimal dose as antigenotoxic agent by interaction between quercetin with CYP 3A4 based on molecular docking.Keywords: antigenotoxic, Carica papaya L., MNPCE, in vivo

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Kementerian Kesehatan Republik Indonesia
  • Universitas Jenderal Achmad Yani
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Edy MeiyantoUniversitas Gadjah Mada14
Riris Istighfari JenieUniversitas Gadjah Mada8
Adam HermawanUniversitas Gadjah Mada5
Asri Mega PutriUniversitas Gadjah Mada3
Prisnu TirtanirmalaUniversitas Gadjah Mada3
Sari HaryantiKementerian Kesehatan Republik Indonesia2
Suwijiyo PramonoUniversitas Gadjah Mada2
Yogi ErtantoUniversitas Gadjah Mada2
Lailatul QodriaUniversitas Gadjah Mada2
Indah HairunisaUniversitas Gadjah Mada2
Ratna Dwi RamadaniNational Research and Innovation Agency2
Annisa NovarinaUniversitas Gadjah Mada2
Rahmawaty RachmadyUniversitas Gadjah Mada2
Idlohatud DilalahUniversitas Gadjah Mada2
Ratna Asmah SusidartiUniversitas Gadjah Mada1
Yoce ApriantoUniversitas Gadjah Mada1
Hilyatul FadliyahUniversitas Gadjah Mada1
Dwi SulistyowatiUniversitas Gadjah Mada1
Laeli MuntafiahUniversitas Gadjah Mada1
Murah Riski Novi AsshagabUniversitas Gadjah Mada1
Nindi WulandariUniversitas Gadjah Mada1
Fikriansyah FikriansyahUniversitas Gadjah Mada1
Mentari WidiastutiUniversitas Gadjah Mada1
Alifia Brilliani HidayahUniversitas Gadjah Mada1
Nuqya AshfannadaUniversitas Gadjah Mada1
Aisyah Nur KhasanahUniversitas Gadjah Mada1
Siti Nur AnnisaUniversitas Gadjah Mada1
Ghea Rachella TiffanyUniversitas Gadjah Mada1
Latifa AmaliaUniversitas Gadjah Mada1
Triana HertianiUniversitas Gadjah Mada1
Kurnia Rahayu PurnomoUniversitas Jenderal Achmad Yani1
Herwandhani PutriUniversitas Gadjah Mada1
Gagas Pradani Nur IlmawatiUniversitas Gadjah Mada1
Mila HanifaUniversitas Gadjah Mada1
Ria Fajarwati KastianNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Antigenotoxicity Activity of Papaya (Carica papaya L.) Leaf Ethanolic Extract on Swiss Mice Induced Cyclophosphamide through Mammalian In Vivo Micronucleus Test</dc:title>
	<dc:creator>Shabrina, Bani Adlina</dc:creator>
	<dc:creator>Juansa, Juang</dc:creator>
	<dc:creator>Putri, Nindya Budiana</dc:creator>
	<dc:creator>Utomo, Rohmad Yudi</dc:creator>
	<dc:creator>Murwanti, Retno</dc:creator>
	<dc:description xml:lang="en-US">Cyclophosphamide (CPA) is an effective chemotherapeutic agent, but has side effect, causing DNA damage (genotoxic). Papaya leaf (Carica papaya L.) is known has flavonoid compound, quercetin. Quercetin is known has DNA protecting effect (antigenotoxic effect) by metabolism modulation. Thus, the aim of this research is to investigate the antigenotoxic effect of ethanolic extract of papaya (Carica papaya L.) leaf (EEPL) on CPA induced mice. The antigenotoxic effect was evaluated by mammalian in vivo micronucleus test. EEPL was orally administered as single treatment at dose 1000 mg/kgBW and in combination with CPA 50 mg/kgBW at dose 250 mg/kgBW; 500 mg/kgBW; and 1000 mg/kgBW. Molecular docking using PLANTS on CYP 3A4 was performed to explore the antigenotoxic effect mechanism. The three different combination dose of EEPL with CPA significantly (P&lt;0.05) decreased the amount of micronucleated polychromatic erytrhocyte (MNPCE)/1000 polychromatic erythrocyte (PCE) and also increased % PCE/(PCE+normochromatic erythrocyte (NCE)), compared with single dose of CPA. Nevertheless, the antigenotoxic effect wasn’t significant compared with each combination dose. The docking score result showed quercetin (-82,41) has more potent interaction to CYP 3A4 than cyclophosphamide (-70,16) and both of them has similar active site at amino acid residue Ile 369 and Thr 309. The results obtained indicated that EEPL at dose 250 mg/KgBB is the optimal dose as antigenotoxic agent by interaction between quercetin with CYP 3A4 based on molecular docking.Keywords: antigenotoxic, Carica papaya L., MNPCE, in vivo</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-02-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/100</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev7iss1pp31-37</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 7, No 1 (2016); 31-37</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/100/100</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>