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Identification and antioxidant activity test of β-tocopherol from Dompu corn oil as anti-aging

International Journal of Advances in Applied Sciences · Vol. 14 No. 1 (2025) · hlm. 175–184

DOI
10.11591/ijaas.v14.i1.pp175-184
ISSN
2722-2594, 2252-8814
Terbit
2025-03-01
Penerbit
Institute of Advanced Engineering and Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Corn is a well-cultivated food crop that is grown extensively worldwide. The used portion of corn is its seeds, which are rich in oil. The objective of this study is to separate and characterize tocopherol from corn oil in Dompu, Sumbawa by employing spectroscopic techniques such as ultraviolet (UV) spectrophotometry, ultra-performance liquid chromatography (UPLC), nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Subsequently, the in-vitro antioxidant activity of the tocopherol was assessed. 1 kilogram of dry maize kernels subjected to 70% ethanol extraction yielded 30 grams (35 ml) of corn oil. The purified isolate obtained from the fractionated extract, using radial chromatography, demonstrated the presence of tocopherol. The isolated sample exhibited the presence of beta-tocopherol. Beta-tocopherol's anti-aging properties were assessed by conducting an in-vitro antioxidant activity test utilizing the tyrosinase enzyme. The IC50 value obtained was 83.954±2.849 ppm. The IC50 value indicates that beta-tocopherol possesses significant antioxidant activity, making it suitable for usage as a primary ingredient in cosmetics and pharmaceutical products.

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  • Universitas Ahmad Dahlan
  • Yogyakarta State University
  • Cenderawasih University
  • Universitas Gadjah Mada
  • Universiti Malaysia Pahang Al-Sultan Abdullah
  • SUNY New Paltz
  • Bandung Institute of Technology
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Ibdal SatarUniversitas Ahmad Dahlan3
Apriyana IrjayantiCenderawasih University2
Maxsi IrmantoCenderawasih University2
Totok Eka SuhartoUniversitas Ahmad Dahlan2
Joko PitoyoUniversitas Ahmad Dahlan2
Trianik WidyaningrumUniversitas Ahmad Dahlan2
Jihan Srikandhia PurnamaAfiliasi tidak diketahui1
Yusni NuryantiAfiliasi tidak diketahui1
Rizki Dika AgustinAfiliasi tidak diketahui1
Ririn DesrianiAfiliasi tidak diketahui1
Mega Utami PutriAfiliasi tidak diketahui1
Mona Safitri FatiahCenderawasih University1
Tri Furianto WibowoThe University of Queensland1
Eko Swi DamarwanYogyakarta State University1
Aulia Farhad Tria Nur AbroriYogyakarta State University1
Alex Sandria Jaya WardhanaYogyakarta State University1
Raymond R. TjandrawinataAtma Jaya Catholic University of Indonesia1
Agung Endro NugrohoUniversitas Gadjah Mada1
Suwijiyo PramonoUniversitas Gadjah Mada1
Novia Tri AstutiUniversitas Gadjah Mada1
Mutiara Wilson PutriUniversitas Ahmad Dahlan1
Muhammad Ali AkbarAfiliasi tidak diketahui1
Lukhi Mulia ShitophytaUniversitas Ahmad Dahlan1
Gita Indah BudiartiUniversitas Ahmad Dahlan1
Endah SulistiawatiUniversitas Ahmad Dahlan1
Rachma Tia EvitasariUniversitas Ahmad Dahlan1
Ika Dyah KumalasariUniversitas Ahmad Dahlan1
Nazira MahmudUniversiti Malaysia Pahang Al-Sultan Abdullah1
Nor Adila Mhd OmarUniversiti Malaysia Pahang Al-Sultan Abdullah1
Herman Dwi SurjonoYogyakarta State University1
Muchlas MuchlasUniversitas Ahmad Dahlan1

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Sumber metadata: OAI-PMH (metadata jurnal) http://ijaas.iaescore.com/index.php/IJAAS/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Identification and antioxidant activity test of β-tocopherol from Dompu corn oil as anti-aging</dc:title>
	<dc:creator>Permadi, Adi</dc:creator>
	<dc:creator>Budiastuti, Pramudita</dc:creator>
	<dc:creator>Setyanto, Barry Nur</dc:creator>
	<dc:creator>Wulandana, Rachmadian</dc:creator>
	<dc:creator>Nuraisyah, Fatma</dc:creator>
	<dc:creator>Novitasari, Putri Rachma</dc:creator>
	<dc:creator>Syamsuddin, Arief</dc:creator>
	<dc:creator>Ramadhan, Muhammad Kaisar Sutomo</dc:creator>
	<dc:creator>Rosandy, Andi Rifki</dc:creator>
	<dc:description xml:lang="en-US">Corn is a well-cultivated food crop that is grown extensively worldwide. The used portion of corn is its seeds, which are rich in oil. The objective of this study is to separate and characterize tocopherol from corn oil in Dompu, Sumbawa by employing spectroscopic techniques such as ultraviolet (UV) spectrophotometry, ultra-performance liquid chromatography (UPLC), nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Subsequently, the in-vitro antioxidant activity of the tocopherol was assessed. 1 kilogram of dry maize kernels subjected to 70% ethanol extraction yielded 30 grams (35 ml) of corn oil. The purified isolate obtained from the fractionated extract, using radial chromatography, demonstrated the presence of tocopherol. The isolated sample exhibited the presence of beta-tocopherol. Beta-tocopherol's anti-aging properties were assessed by conducting an in-vitro antioxidant activity test utilizing the tyrosinase enzyme. The IC50 value obtained was 83.954±2.849 ppm. The IC50 value indicates that beta-tocopherol possesses significant antioxidant activity, making it suitable for usage as a primary ingredient in cosmetics and pharmaceutical products.</dc:description>
	<dc:publisher xml:lang="en-US">Institute of Advanced Engineering and Science</dc:publisher>
	<dc:contributor xml:lang="en-US">Universitas Ahmad Dahlan</dc:contributor>
	<dc:date>2025-03-01</dc:date>
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	<dc:identifier>https://ijaas.iaescore.com/index.php/IJAAS/article/view/21180</dc:identifier>
	<dc:identifier>10.11591/ijaas.v14.i1.pp175-184</dc:identifier>
	<dc:source xml:lang="en-US">International Journal of Advances in Applied Sciences; Vol 14, No 1: March 2025; 175-184</dc:source>
	<dc:source>2722-2594</dc:source>
	<dc:source>2252-8814</dc:source>
	<dc:source>10.11591/ijaas.v14.i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijaas.iaescore.com/index.php/IJAAS/article/view/21180/13233</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Institute of Advanced Engineering and Science (IAES)</dc:rights>
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