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Ligninolitic Enzyme Immobilization from Pleurotus ostreatus for Dye and Batik Wastewater Decolorization

Jurnal Pendidikan IPA Indonesia · Vol. 8 No. 2 (2019) · hlm. 220–229

DOI
10.15294/jpii.v8i2.19372
ISSN
2089-4392, 2339-1286
Terbit
2019-06-28
Penerbit
Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)
Bahasa
eng

Abstrak

Batik wastewater is one of the environmental problems which has become a significant water pollution problem recently. It contains a large variety of synthetic dyes, chemicals, and has high chemical oxygen demand (COD). Synthetic dyes are difficult to degrade and have recalcitrant and toxic characteristics. Dyes can be degraded by ligninolytic enzymes, including laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP). The immobilized ligninolytic enzyme is effectively used to enhance the degradation of dye wastewater. Pleurotus ostreatus, the most abundant ligninolytic enzyme source, is the candidate to regenerate fungal biomass as the bioremediation agent. Immobilization of ligninolytic enzyme using alginate was observed in this study for its efficiency to decolorize and decrease COD concentration of RBBR dye and Naphtol batik wastewater in different time periods (0, 2, 4, 6, 24, 48, 72 h) and growth conditions (static and agitated). The results show that the Lac, MnP, LnP activities of P. ostreatus are 200.43, 9.714, 12938.60 U/l, respectively. Static conditions within 48 hours exhibit the highest percentage of decolorization of dye. Ligninolytic immobilized from its condition has decolorized RBBR dye up to 75.88%, while the percentage of decolorization of agitated culture is 68.09%. This ligninolytic immobilized has decolorized about 94.867% of batik wastewater within 24 hours under a static condition. It is also able to decrease the COD level of the batik wastewater containing Naphtol dye (504 to 233 mg/l) within 24 hours under a shaking condition. Immobilization of enzyme has been a promising alternative for decolorization of dye and batik wastewater.

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  • Indonesian Institute of Sciences
  • Jenderal Soedirman University
  • National Research and Innovation Agency
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Endang MugiastutiAfiliasi tidak diketahui2
Ruth Feti RahayuniatiAfiliasi tidak diketahui2
Loekas SoesantoAfiliasi tidak diketahui2
Khusnul KhotimahNational Research and Innovation Agency1
Triastuti SulistyaningsihAfiliasi tidak diketahui1
Luigy Maurizka YasmineAfiliasi tidak diketahui1
Erni MartaniAfiliasi tidak diketahui1
Rina Sri KasiamdariAfiliasi tidak diketahui1
Yekti Asih PurwestriAfiliasi tidak diketahui1
Putri RamadaniAfiliasi tidak diketahui1
Saefuddin AzizAfiliasi tidak diketahui1
ABDUL MANANAfiliasi tidak diketahui1
Purwati PurwatiAfiliasi tidak diketahui1
Mardiyah KurniasihAfiliasi tidak diketahui1
Kasandra BudiarniAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/nju/index.php/jpii/oai, pertama di-harvest 29 Okt 2026, terakhir terlihat 1 Nov 2026.
Metadata asli (oai_dc)
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	<dc:title xml:lang="en-US">Ligninolitic Enzyme Immobilization from Pleurotus ostreatus for Dye and Batik Wastewater Decolorization</dc:title>
	<dc:creator>Dewi, R. S.</dc:creator>
	<dc:creator>Ilyas, M.</dc:creator>
	<dc:creator>Sari, A. A.</dc:creator>
	<dc:subject xml:lang="en-US">COD, decolorization, immobilization, ligninolytic, Pleurotus ostreatus</dc:subject>
	<dc:description xml:lang="en-US">Batik wastewater is one of the environmental problems which has become a significant water pollution problem recently. It contains a large variety of synthetic dyes, chemicals, and has high chemical oxygen demand (COD). Synthetic dyes are difficult to degrade and have recalcitrant and toxic characteristics. Dyes can be degraded by ligninolytic enzymes, including laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP). The immobilized ligninolytic enzyme is effectively used to enhance the degradation of dye wastewater. Pleurotus ostreatus, the most abundant ligninolytic enzyme source, is the candidate to regenerate fungal biomass as the bioremediation agent. Immobilization of ligninolytic enzyme using alginate was observed in this study for its efficiency to decolorize and decrease COD concentration of RBBR dye and Naphtol batik wastewater in different time periods (0, 2, 4, 6, 24, 48, 72 h) and growth conditions (static and agitated). The results show that the Lac, MnP, LnP activities of P. ostreatus are 200.43, 9.714, 12938.60 U/l, respectively. Static conditions within 48 hours exhibit the highest percentage of decolorization of dye. Ligninolytic immobilized from its condition has decolorized RBBR dye up to 75.88%, while the percentage of decolorization of agitated culture is 68.09%. This ligninolytic immobilized has decolorized about 94.867% of batik wastewater within 24 hours under a static condition. It is also able to decrease the COD level of the batik wastewater containing Naphtol dye (504 to 233 mg/l) within 24 hours under a shaking condition. Immobilization of enzyme has been a promising alternative for decolorization of dye and batik wastewater.</dc:description>
	<dc:publisher xml:lang="en-US">Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)</dc:publisher>
	<dc:contributor xml:lang="en-US">2Research Center for Chemistry, Indonesian Institute of Sciences</dc:contributor>
	<dc:date>2019-06-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/nju/jpii/article/view/19372</dc:identifier>
	<dc:identifier>10.15294/jpii.v8i2.19372</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Pendidikan IPA Indonesia; Vol 8, No 2 (2019): June 2019; 220-229</dc:source>
	<dc:source>2089-4392</dc:source>
	<dc:source>2339-1286</dc:source>
	<dc:source>10.15294/jpii.v8i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/view/19372/9368</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/19372/3976</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/19372/3977</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/19372/3978</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/19372/3981</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/19372/4031</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/19372/4078</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Jurnal Pendidikan IPA Indonesia</dc:rights>
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