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Identification of Metallothionein Gene in Human Plasma: A Molecular Analysis of Cadmium and Lead Pollution in Gas Station Environment

Jurnal Pendidikan IPA Indonesia · Vol. 7 No. 4 (2018) · hlm. 383–390

DOI
10.15294/jpii.v7i4.15487
ISSN
2089-4392, 2339-1286
Terbit
2018-12-28
Penerbit
Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)
Bahasa
eng

Abstrak

Metallothionein (MTs) is a protein that binds to metals, cysteine-rich and has low molecular weights. Metallothionein binds metals and has some essential cellular functions, such as transportation, storage, and metal detoxification. When metallic lead (Pb) and cadmium (Cd) are absorbed, it will immediately be bounded by metallothionein and transported by the blood to the liver. Metallothionein gene is encoded by MTs. This study aimed to identify the MTs gene in blood of fuel station workers which can further be used as a biomarker of the vulnerability of the body as a result of exposure to heavy metals like Pb and Cd in humans. This study was a cross-sectional study, conducted for 8 months and took place in Fuel Stations in the city of Semarang. Samples were fuel station workers who met the study criteria, they sought to fulfill as much as 52 samples. Blood of samples was taken approximately 5 ml and then the MT2 gene was analyzed using Polymerase Chain Reaction (PCR). The data were analyzed descriptively. The results showed that 96% of MT2 gene expression had a resemblance to normal human gene sequences by Blast GenBank. MT2 gene expression does not always reflect the accumulation of lead and cadmium in the blood of fuel station workers. The MT2 gene is likely to have a limitation when used as a biomarker for early detection of the heavy metals exposure in humans to determine the susceptibility of heavy metals exposure.

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  • State University of Semarang
  • Diponegoro University
  • Universitas Muhammadiyah Semarang
  • Hasanuddin University
  • Nagoya University
  • University of Malaya
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Rekan penulisOrganisasiArtikel bersama
R. SusantiState University of Semarang5
Widya Hary CahyatiState University of Semarang3
Oktia Woro Kasmini HandayaniState University of Semarang3
Mahalul AzamState University of Semarang3
Sri Ratna RahayuState University of Semarang2
Retno Sri IswariState University of Semarang2
Yanuarita TursinawatiUniversitas Muhammadiyah Semarang2
Wiwi IsnaeniState University of Semarang2
Lisdiana LisdianaState University of Semarang2
Intan ZainafreeState University of Semarang2
Dewi MustikaningtyasState University of Semarang2
Muchamad DafipState University of Semarang2
Suryani As’ádHasanuddin University1
Sri Achadi NugraheniDiponegoro University1
Pingkan FristiwiDiponegoro University1
Fatimah Asri Fadhilah AuliaDiponegoro University1
Shafa Alya KamilaDiponegoro University1
Widowati SaputraState University of Semarang1
RR. Sri Ratna RahayuAfiliasi tidak diketahui1
Sumarti SumartiState University of Semarang1
Sri Ratna RahayuState University of Semarang1
Salsabila Kinaya Pranindita LalaState University of Semarang1
R SusantiState University of Semarang1
LisdianaState University of Semarang1
Revinta Nur Aulia RochlyState University of Semarang1
Nugrahaningsih WHState University of Semarang1
Nugrahaningsih WHState University of Semarang1
Khoerina SalwaState University of Semarang1
Didik Huswo UtomoNagoya University1
W H NugrahaningsihState University of Semarang1
Wulan ChristijantiState University of Semarang1
Sujatha RamasamyUniversity of Malaya1
Vivi Anggraeni LaraswatiState University of Semarang1
Siti Rafika PutriState University of Semarang1
Chatila MaharaniState University of Semarang1
Anggit KartikasariState University of Semarang1
Arum KartikadewiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/nju/index.php/jpii/oai, pertama di-harvest 29 Okt 2026, terakhir terlihat 1 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Identification of Metallothionein Gene in Human Plasma: A Molecular Analysis of Cadmium and Lead Pollution in Gas Station Environment</dc:title>
	<dc:creator>Dewi, N. K.</dc:creator>
	<dc:creator>Yuniastuti, A.</dc:creator>
	<dc:creator>Ahmed, A. M. A.</dc:creator>
	<dc:subject xml:lang="en-US">biomarker, metallothioenian gene, lead, cadmium</dc:subject>
	<dc:description xml:lang="en-US">Metallothionein (MTs) is a protein that binds to metals, cysteine-rich and has low molecular weights. Metallothionein binds metals and has some essential cellular functions, such as transportation, storage, and metal detoxification. When metallic lead (Pb) and cadmium (Cd) are absorbed, it will immediately be bounded by metallothionein and transported by the blood to the liver. Metallothionein gene is encoded by MTs. This study aimed to identify the MTs gene in blood of fuel station workers which can further be used as a biomarker of the vulnerability of the body as a result of exposure to heavy metals like Pb and Cd in humans. This study was a cross-sectional study, conducted for 8 months and took place in Fuel Stations in the city of Semarang. Samples were fuel station workers who met the study criteria, they sought to fulfill as much as 52 samples. Blood of samples was taken approximately 5 ml and then the MT2 gene was analyzed using Polymerase Chain Reaction (PCR). The data were analyzed descriptively. The results showed that 96% of MT2 gene expression had a resemblance to normal human gene sequences by Blast GenBank. MT2 gene expression does not always reflect the accumulation of lead and cadmium in the blood of fuel station workers. The MT2 gene is likely to have a limitation when used as a biomarker for early detection of the heavy metals exposure in humans to determine the susceptibility of heavy metals exposure.</dc:description>
	<dc:publisher xml:lang="en-US">Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2018-12-28</dc:date>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://journal.unnes.ac.id/nju/jpii/article/view/15487</dc:identifier>
	<dc:identifier>10.15294/jpii.v7i4.15487</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Pendidikan IPA Indonesia; Vol 7, No 4 (2018): December 2018; 383-390</dc:source>
	<dc:source>2089-4392</dc:source>
	<dc:source>2339-1286</dc:source>
	<dc:source>10.15294/jpii.v7i4</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/view/15487/8675</dc:relation>
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	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/15487/3303</dc:relation>
	<dc:relation>https://journal.unnes.ac.id/nju/jpii/article/downloadSuppFile/15487/3304</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Jurnal Pendidikan IPA Indonesia</dc:rights>
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