Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste

Amekan, Yumechris; Wangi, Dyah Sekar A P; Cahyanto, Muhammad Nur; Sarto, Sarto; Widada, Jaka

doi:10.14710/ijred.7.2.101-109

Akses terbuka Jurnal SINTA 1

Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste

International Journal of Renewable Energy Development : JRED · Vol. 7 No. 2 (2018) · hlm. 101–109

DOI: 10.14710/ijred.7.2.101-109
ISSN: 2252-4940
Terbit: 2018-07-10
Penerbit: Center of Biomass & Renewable Energy (CBIORE)
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-sa/4.0

Abstrak

The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37°C and initial pH 7.05 ± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10

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Universitas Gadjah Mada
Jenderal Soedirman University
Sebelas Maret University
The University of Tokyo
IPB University
Institut Teknologi Sepuluh Nopember
Universitas Muhammadiyah Semarang
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Rekan penulis	Organisasi	Artikel bersama
Siti Kabirun	Universitas Gadjah Mada	3
Irfan Dwidya Prijambada	Universitas Gadjah Mada	3
Tri Wibawa	Universitas Gadjah Mada	2
Nastiti Wijayanti	Universitas Gadjah Mada	2
Endang Semiarti	Universitas Gadjah Mada	2
N. Ngadiman	Universitas Gadjah Mada	2
Reni Nurjasmi	Afiliasi tidak diketahui	2
Widya Asmara	Universitas Gadjah Mada	2
Alimuddin Alimuddin	IPB University	2
Donny Widianto	Universitas Gadjah Mada	2
Sri Wedhastri	Universitas Gadjah Mada	2
Diani Mentari	Jenderal Soedirman University	1
Sudadi Sudadi	Sebelas Maret University	1
Sumarno Sumarno	Institut Teknologi Sepuluh Nopember	1
Tri Joko Raharjo	State University of Semarang	1
Stalis Norma Ethica	Universitas Muhammadiyah Semarang	1
Oedjijono Oedjijono	Jenderal Soedirman University	1
Nur Fitriana Ariyanti	Universitas Gadjah Mada	1
Siti Subandiyah	Universitas Gadjah Mada	1
Eka Tarwaca Susila Putra	Universitas Gadjah Mada	1
Sukmana Siswandana Putra	Universitas Gadjah Mada	1
Bambang Hendro Sunarminto	Universitas Gadjah Mada	1
Mujiyo Mujiyo	Sebelas Maret University	1
Eko Hanudin	Universitas Gadjah Mada	1
Murwantoko Murwantoko	Universitas Gadjah Mada	1
Alim Isnansetyo	Universitas Gadjah Mada	1
Melki Melki	Sriwijaya University	1
Masaya Nishiyama	Afiliasi tidak diketahui	1
Dolly Iriani Damarjaya	Universitas Gadjah Mada	1
Keishi Senoo	The University of Tokyo	1
Shigeto Otsuka	The University of Tokyo	1
Edy Meiyanto	Universitas Gadjah Mada	1
Yuyun Farida	Afiliasi tidak diketahui	1
M. Murwantoko	Afiliasi tidak diketahui	1
Yani Lestari Nuraini	Afiliasi tidak diketahui	1

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Metadata asli (oai_dc)

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	<dc:title xml:lang="en-US">Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste</dc:title>
	<dc:creator>Amekan, Yumechris</dc:creator>
	<dc:creator>Wangi, Dyah Sekar A P</dc:creator>
	<dc:creator>Cahyanto, Muhammad Nur</dc:creator>
	<dc:creator>Sarto, Sarto</dc:creator>
	<dc:creator>Widada, Jaka</dc:creator>
	<dc:subject xml:lang="en-US">Inoculum; Biohydrogen; Melon fruit waste; Dark Fermentation; DGGE</dc:subject>
	<dc:description xml:lang="en-US">The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37°C and initial pH 7.05 ± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10</dc:description>
	<dc:publisher xml:lang="en-US">Center of Biomass &amp; Renewable Energy (CBIORE)</dc:publisher>
	<dc:contributor xml:lang="en-US">This study was supported by Indonesia Endowment Fund For Education (LPDP) to the first author and Short-Term Exchange Program in science and engineering at Tokyo University of Agriculture and Technology to the second author.</dc:contributor>
	<dc:date>2018-07-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijred.cbiore.id/index.php/ijred/article/view/17464</dc:identifier>
	<dc:identifier>10.14710/ijred.7.2.101-109</dc:identifier>
	<dc:source xml:lang="en-US">International Journal of Renewable Energy Development; Vol 7, No 2 (2018): July 2018; 101-109</dc:source>
	<dc:source>2252-4940</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijred.cbiore.id/index.php/ijred/article/view/17464/pdf</dc:relation>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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