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Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1

International Journal of Renewable Energy Development : JRED · Vol. 10 No. 4 (2021) · hlm. 857–863

DOI
10.14710/ijred.2021.38887
ISSN
2252-4940
Terbit
2021-11-01
Penerbit
Center of Biomass & Renewable Energy (CBIORE)
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

In recent years, attention to microbial dehalogenase has continually increased due to its potential application, both in bioremediation and in the biosynthesis of fine chemicals. Many microbial recombinant strains carrying dehalogenase gene have been developed, particularly to increase the dehalogenase production and its quality. In this study, we aimed to find the optimum condition for the production of active haloacid dehalogenase by E. coli BL21 (DE3) harboring recombinant plasmid pET-bcfd1 that carried haloacid dehalogenase gene from Bacillus cereus IndB1 local strain. This would be examined by assessing the ability of whole cell life culture to degrade monochloroacetic acid (MCA) and quantifying the chloride ion released into the medium. Several variables were evaluated to find this optimal condition. We found that the best condition for MCA biodegradation using this recombinant clone was at 0.2 mM MCA, 10 μM of isopropyl β-D-1-thiogalactopyranoside (IPTG), 6 hours of pre-induction incubation at 37ºC with shaking, 2 hours IPTG induction at 30ºC with shaking, at pH 7 in Luria Bertani (LB) liquid medium without NaCl, which produced about 0.056 mM chloride ions. Inducer concentration, pre-induction incubation time and temperature, as well as induction time and temperature were apparent to be associated with the expression of the protein, while the MCA concentration and the pH of the medium influenced the ability of the recombinant E. coli BL21 (DE3)/pET-bcfd1 to grow in toxic environment. Our findings laid the foundation for exploration of dehalogenases from local Bacillus strains through genetic engineering for MCA biodegradation

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  • Bandung Institute of Technology
  • Indonesian Institute of Sciences
  • IPB University
  • National Research and Innovation Agency
  • Yogyakarta State University
  • State University of Semarang
  • Universitas Islam Negeri Sultan Maulana Hasanuddin Banten
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Rekan penulisOrganisasiArtikel bersama
Rukman HertadiBandung Institute of Technology2
Eli RohaetiYogyakarta State University2
Cynthia Linaya RadimanBandung Institute of Technology2
N. M. SurdiaBandung Institute of Technology2
Supartono SupartonoState University of Semarang1
Sadijah AchmadBandung Institute of Technology1
Oei Ban LiangBandung Institute of Technology1
Zuhdina SabiqohBandung Institute of Technology1
Masrukhin MasrukhinNational Research and Innovation Agency1
Suryo WiyonoIPB University1
I Made SudianaIndonesian Institute of Sciences1
Atit KantiIndonesian Institute of Sciences1
Wawan SetiawanIPB University1
Toga Pangihotan NapitupuluNational Research and Innovation Agency1
Efi Toding TondokIPB University1
I Nyoman SumertaNational Research and Innovation Agency1
Ridani Rino AnggoroBandung Institute of Technology1
Ni Putu YuliastriBandung Institute of Technology1
Idris IdrisUniversitas Islam Negeri Sultan Maulana Hasanuddin Banten1
K. KhoiruddinBandung Institute of Technology1
I Gede WentenBandung Institute of Technology1
Putu Teta Prihartini AryantiUniversitas Jenderal Achmad Yani1
Anita Kusuma WardaniBandung Institute of Technology1
Grandprix T.M. KadjaBandung Institute of Technology1
Nurul Faiqotul HimmaUniversity of Brawijaya1
Nicholaus PrasetyaBandung Institute of Technology1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijred.cbiore.id/index.php/ijred/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1</dc:title>
	<dc:creator>Ratnaningsih, Enny</dc:creator>
	<dc:creator>Ade, Rachmad</dc:creator>
	<dc:creator>Putri, Rindia Maharani</dc:creator>
	<dc:creator>Idris, Idris</dc:creator>
	<dc:subject xml:lang="en-US">biodegradation; haloacid dehalogenase; monochloroacetic acid</dc:subject>
	<dc:description xml:lang="en-US">In recent years, attention to microbial dehalogenase has continually increased due to its potential application, both in bioremediation and in the biosynthesis of fine chemicals. Many microbial recombinant strains carrying dehalogenase gene have been developed, particularly to increase the dehalogenase production and its quality. In this study, we aimed to find the optimum condition for the production of active haloacid dehalogenase by E. coli BL21 (DE3) harboring recombinant plasmid pET-bcfd1 that carried haloacid dehalogenase gene from Bacillus cereus IndB1 local strain. This would be examined by assessing the ability of whole cell life culture to degrade monochloroacetic acid (MCA) and quantifying the chloride ion released into the medium. Several variables were evaluated to find this optimal condition. We found that the best condition for MCA biodegradation using this recombinant clone was at 0.2 mM MCA, 10 μM of isopropyl β-D-1-thiogalactopyranoside (IPTG), 6 hours of pre-induction incubation at 37ºC with shaking, 2 hours IPTG induction at 30ºC with shaking, at pH 7 in Luria Bertani (LB) liquid medium without NaCl, which produced about 0.056 mM chloride ions. Inducer concentration, pre-induction incubation time and temperature, as well as induction time and temperature were apparent to be associated with the expression of the protein, while the MCA concentration and the pH of the medium influenced the ability of the recombinant E. coli BL21 (DE3)/pET-bcfd1 to grow in toxic environment. Our findings laid the foundation for exploration of dehalogenases from local Bacillus strains through genetic engineering for MCA biodegradation</dc:description>
	<dc:publisher xml:lang="en-US">Center of Biomass &amp; Renewable Energy (CBIORE)</dc:publisher>
	<dc:date>2021-11-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijred.cbiore.id/index.php/ijred/article/view/38887</dc:identifier>
	<dc:identifier>10.14710/ijred.2021.38887</dc:identifier>
	<dc:source xml:lang="en-US">International Journal of Renewable Energy Development; Vol 10, No 4 (2021): November 2021; 857-863</dc:source>
	<dc:source>2252-4940</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijred.cbiore.id/index.php/ijred/article/view/38887/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 The Authors. Published by Centre of Biomass and Renewable Energy (CBIORE)</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>