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KEANEKARAGAMAN DAN KEKERABATAN LALAT BUAH (DIPTERA: TEPHRITIDAE) DI KALIMANTAN SELATAN BERDASARKAN KARAKTER MORFOLOGI DAN MOLEKULAR (RAPD-PCR DAN SEKUENSING DNA)

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 13 No. 2 (2014) · hlm. 192–202

DOI
10.23960/j.hptt.213192-202
ISSN
2461-0399, 1411-7525
Terbit
2014-08-25
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Diversity and phylogeny of fruit fly (Diptera: Tephritidae) in South Kalimantan based on morphology and molecular (RAPD-PCR and DNA sequencing). Seven species of fruit fly was known by morphological identification. The fruit flies were found from trapping with methyl eugenol and fruit collecting at all study sites in South Kalimantan. The results showed that as much as 17 plants were infected by fruit fly. Dendrogram based on morphological identification analyzed by using UPGMA with MEGA 4 program consisted in a group consisting of 5 sub-groups. Bactrocera carambolae and Bactrocera papayae of morphology were still a closely related fruit fly at 0.935. Whereas, based on RAPD result analized by UPGMA using 20 character of DNA based, showed that out of seven species consisted 2 groups, 1st group were B. umbrosa, B. occipitalis and sub-group of B. latifrons. The second group consists of sub-groups B.carambolae, B. papaya, sub-group B. albistrigata and B. cucurbitae. The results of dendrogram from sequencing DNA fruit fly analysis comprised one of group and three sub-groups. The first sub-groups were B. papayae, B. carambolae, B. occipitalis, B.latifrons. The second subgroup were B. cucurbitae and B. umbrosa. While B. albistrigata separate but still one group with another fruit flies. The results of DNA sequencing showed that there were a homology of the seven species of the fruit fly i.e at 83 base pair / bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A ). The length of the base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons were respectively 615, 898, 570.969, 898 and 615 bp. The results of morphological analysis and RAPD methods showed difference in the distribution of groups and sub-groups. But based on morphologycal and DNA identification seven species of fruit flies found were all same as the genebank.

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Aminudin AfandhiAfiliasi tidak diketahui3
Akhmad RizaliAfiliasi tidak diketahui3
Toto HimawanAfiliasi tidak diketahui3
Yogo SetiawanAfiliasi tidak diketahui2
Rina RachmawatiAfiliasi tidak diketahui2
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BANDUNG SAHARIAfiliasi tidak diketahui2
Ima FitrianiAfiliasi tidak diketahui2
Marianus MarianusAfiliasi tidak diketahui1
Sumeru AshariAfiliasi tidak diketahui1
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Mahanani Tri AsriAfiliasi tidak diketahui1
Siti Rasminah ChaelaniAfiliasi tidak diketahui1
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David SoetjiptoAfiliasi tidak diketahui1
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Arif Danu SatriaAfiliasi tidak diketahui1
Nurul QomariyahAfiliasi tidak diketahui1
Yuliantoro BaliadiAfiliasi tidak diketahui1
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Ika Rochdjatun SastrahidayatAfiliasi tidak diketahui1
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Etik Mar`ati AchadianAfiliasi tidak diketahui1
Achmad Fitriadi TaufiqurrahmanAfiliasi tidak diketahui1
Mohamad Rizkiy HidayatAfiliasi tidak diketahui1
Ito FernandoUniversity of Brawijaya1
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Anisya Putri KarinaAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">KEANEKARAGAMAN DAN KEKERABATAN LALAT BUAH (DIPTERA: TEPHRITIDAE) DI KALIMANTAN SELATAN BERDASARKAN KARAKTER MORFOLOGI DAN MOLEKULAR (RAPD-PCR DAN SEKUENSING DNA)</dc:title>
	<dc:creator xml:lang="en">Pramudi, M Indar</dc:creator>
	<dc:creator xml:lang="en">Puspitarini, Retno Dyah</dc:creator>
	<dc:creator xml:lang="en">Rahardjo, Bambang Tri</dc:creator>
	<dc:subject xml:lang="en">DNA sequencing</dc:subject>
	<dc:subject xml:lang="en">fruit fly</dc:subject>
	<dc:subject xml:lang="en">morphology</dc:subject>
	<dc:subject xml:lang="en">PCR-RAPD</dc:subject>
	<dc:subject xml:lang="en">South Kalimantan</dc:subject>
	<dc:description xml:lang="en">Diversity and phylogeny of fruit fly (Diptera: Tephritidae) in South Kalimantan based on morphology and molecular (RAPD-PCR and DNA sequencing). Seven species of fruit fly was known by morphological identification. The fruit flies were found from  trapping with methyl eugenol and fruit collecting at all study sites in South Kalimantan. The results showed that as much as 17  plants were infected by fruit fly. Dendrogram based on morphological identification analyzed by using UPGMA with MEGA 4 program consisted in a group consisting of 5 sub-groups. Bactrocera carambolae and Bactrocera papayae of morphology were still a closely related fruit fly at 0.935. Whereas, based on RAPD result analized by UPGMA using 20 character of DNA based, showed that out of seven species consisted 2 groups, 1st group were B. umbrosa,  B. occipitalis and sub-group of B. latifrons. The second group consists of sub-groups B.carambolae, B. papaya, sub-group B. albistrigata and B. cucurbitae. The results of dendrogram from sequencing DNA fruit fly analysis comprised one of group and three sub-groups. The first sub-groups were B. papayae, B. carambolae, B. occipitalis, B.latifrons. The second subgroup were B. cucurbitae and B. umbrosa. While B. albistrigata separate but still one group with another fruit flies. The results of DNA sequencing showed that there were a homology of the seven species of the fruit fly i.e at 83 base pair / bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A ). The length of the base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons were respectively 615, 898, 570.969, 898 and 615 bp. The results of morphological analysis and RAPD methods showed difference in the distribution of groups and sub-groups. But based on morphologycal and DNA identification seven species of fruit flies found were all same as the genebank.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2014-08-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/66</dc:identifier>
	<dc:identifier>10.23960/j.hptt.213192-202</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA; 192-202</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/66/64</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2014 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>