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EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 16 No. 2 (2017) · hlm. 115–123

DOI
10.23960/j.hptt.216115-123
ISSN
2461-0399, 1411-7525
Terbit
2017-04-19
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

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24 penulis · 53 tautan kepenulisan bersama · 20 lintas organisasi

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  • Universitas Gadjah Mada
  • Sriwijaya University
  • Universitas Pattimura
  • IPB University
  • Universitas Papua
  • Indonesian Institute of Sciences
  • Field Science Center for Northern Biosphere, Hokkaido University
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  • Organisasi berbeda
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
HarjonoAfiliasi tidak diketahui3
Tri JokoUniversitas Gadjah Mada3
Ani WidiastutiUniversitas Gadjah Mada2
Siti SubandiyahUniversitas Gadjah Mada2
Rachmanto Bambang WijoyoUniversitas Gadjah Mada1
Endang SulistyaningsihUniversitas Gadjah Mada1
Sri BudiartiIPB University1
Sebastian MarginoUniversitas Gadjah Mada1
Susanti TasikUniversitas Papua1
Indira RiastiwiIndonesian Institute of Sciences1
Yuli KristiawatiUniversitas Gadjah Mada1
Yufita Dwi ChintaField Science Center for Northern Biosphere, Hokkaido University1
Zjakiyah Ari SusantiUniversitas Gadjah Mada1
Danar WicaksonoUniversitas Gadjah Mada1
Stephen Mark HarperThe University of Queensland1
Hurin Nabila Aghnia IlmaUniversitas Gadjah Mada1
Annisa Hasta PratiwiUniversitas Gadjah Mada1
Indra YustianSriwijaya University1
Hilda ZulkifliSriwijaya University1
Indah Anggraini YusantiUniversitas PGRI Palembang1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG</dc:title>
	<dc:creator xml:lang="en">Taribuka, Johanna</dc:creator>
	<dc:creator xml:lang="en">Sumardiyono, Christanti</dc:creator>
	<dc:creator xml:lang="en">Widyastuti, Siti Muslimah</dc:creator>
	<dc:creator xml:lang="en">Wibowo, Arif</dc:creator>
	<dc:subject xml:lang="en">Identification</dc:subject>
	<dc:subject xml:lang="en">banana</dc:subject>
	<dc:subject xml:lang="en">Fusarium oxysporum f.sp. cubense</dc:subject>
	<dc:subject xml:lang="en">Trichoderma endophytic</dc:subject>
	<dc:description xml:lang="en">Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2017-04-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/355</dc:identifier>
	<dc:identifier>10.23960/j.hptt.216115-123</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 16 No. 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA; 115-123</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/355/341</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>