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Incidence of main viruses infecting local garlic in Java, Indonesia

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 23 No. 2 (2023) · hlm. 7–15

DOI
10.23960/jhptt.2237-15
ISSN
2461-0399, 1411-7525
Terbit
2023-05-27
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Virus infection is one of the challenges in garlic production due to it perpetuates from one generation to the next and its infection caused huge yield reduction. There was still few information regarding virus status on Indonesian local garlic cultivars. This study was aimed to detect four major viruses infecting local garlic in Indonesia, they were members of genus Potyvirus (Onion yellow dwarf virus/OYDV, Leek yellow stripe virus/LYSV), and Carlavirus (Garlic common laten virus/ GCLV and Shallot latent virus/SLV). Garlic samples were obtained from IPB University collection and field survey in Tegal and Karanganyar (Central Java Province). Dot immuno-binding assay (DIBA) was done for initial virus indexing on non-commercial and commercial cultivars. Reverse transcription polymerase chain reaction (RT-PCR) using four specific primers was done to detect virus on commercial cultivars. DIBA from leaf samples showed that virus incidence of OYDV was relatively higher (92.3 to 100%) than GCLV and SLV (84.6 to 100%) from all tested cultivars. On average, ‘Lumbu Hijau’ has the lowest level of virus titter (severity) than other cultivars. The virus incidence of both bulbil and single clover was similar (97 – 100%) while virus titter of OYDV, GCLV, and SLV on bulbil was the lowest than other propagation materials. Detection by RT-PCR from two commercial cultivars showed that ‘Lumbu Hijau’ has less virus incidence than ‘Jawa Lama’. LYSV, OYDV, GCLV were detected on both cultivars but SLV was not found. Further virus indexing using larger number of samples and involving more virus targets needs to be done.

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  • Universitas Halu Oleo
  • IPB University
  • State University of Jakarta
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
SRIANI SUJIPRIHATIAfiliasi tidak diketahui4
Kikin Hamzah MutaqinIPB University4
Tri Asmira DamayantiAfiliasi tidak diketahui3
Asmar HasanUniversitas Halu Oleo2
MUHAMMAD TAUFIKUniversitas Halu Oleo2
Widodo WidodoState University of Jakarta2
RUSMILAH SUSENOAfiliasi tidak diketahui2
Endang NurhayatiAfiliasi tidak diketahui2
NOOR AIDAWATIAfiliasi tidak diketahui2
Suryo WiyonoAfiliasi tidak diketahui2
John ThomasAfiliasi tidak diketahui2
Purnama HidayatAfiliasi tidak diketahui2
Sofi MawarniAfiliasi tidak diketahui2
Widodo WidodoIPB University1
Muhammad TaufikUniversitas Halu Oleo1
Ahmad FatsanAfiliasi tidak diketahui1
Sudarsono SudarsonoUniversitas Halu Oleo1
Ismail MaskromoAfiliasi tidak diketahui1
Dwi HastutiAfiliasi tidak diketahui1
Ahmad MukoffiAfiliasi tidak diketahui1
Hendrik SuhendriAfiliasi tidak diketahui1
Sri IndahAfiliasi tidak diketahui1
Cut RahmawatiAfiliasi tidak diketahui1
Lia HandayaniAfiliasi tidak diketahui1
Muhamad SyukurAfiliasi tidak diketahui1
Redy GaswantoAfiliasi tidak diketahui1
Bambang Sapta PurwokoAfiliasi tidak diketahui1
Gede SuastikaAfiliasi tidak diketahui1
SIENTJE MANDANG SUMARAWAfiliasi tidak diketahui1
Sri SulandariAfiliasi tidak diketahui1
JUMANTO HARJOSUDARMOAfiliasi tidak diketahui1
SOEMARTONO SOSROMARSONOAfiliasi tidak diketahui1
DWI SUBEKTIAfiliasi tidak diketahui1
ORAWAN CHATCHAWANKAN PANICHAfiliasi tidak diketahui1
Hamdayanty HamdayantyAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Incidence of main viruses infecting local garlic in Java, Indonesia</dc:title>
	<dc:creator xml:lang="en">Hidayat, Sri Hendrastuti</dc:creator>
	<dc:creator xml:lang="en">Meliyana, Meliyana</dc:creator>
	<dc:creator xml:lang="en">Yulianingsih, Refa</dc:creator>
	<dc:creator xml:lang="en">Dinarti, Diny</dc:creator>
	<dc:creator xml:lang="en">Nurulita, Sari </dc:creator>
	<dc:subject xml:lang="en">carlavirus</dc:subject>
	<dc:subject xml:lang="en">dot immuno-binding assay</dc:subject>
	<dc:subject xml:lang="en"> potyvirus</dc:subject>
	<dc:subject xml:lang="en">reverse transcription polymerase chain reaction</dc:subject>
	<dc:description xml:lang="en">Virus infection is one of the challenges in garlic production due to it perpetuates from one generation to the next and its infection caused huge yield reduction. There was still few information regarding virus status on Indonesian local garlic cultivars. This study was aimed to detect four major viruses infecting local garlic in Indonesia, they were members of genus Potyvirus (Onion yellow dwarf virus/OYDV, Leek yellow stripe virus/LYSV), and Carlavirus (Garlic common laten virus/ GCLV and Shallot latent virus/SLV). Garlic samples were obtained from IPB University collection and field survey in Tegal and Karanganyar (Central Java Province). Dot immuno-binding assay (DIBA) was done for initial virus indexing on non-commercial and commercial cultivars. Reverse transcription polymerase chain reaction (RT-PCR) using four specific primers was done to detect virus on commercial cultivars. DIBA from leaf samples showed that virus incidence of OYDV was relatively higher (92.3 to 100%) than GCLV and SLV (84.6 to 100%) from all tested cultivars. On average, ‘Lumbu Hijau’ has the lowest level of virus titter (severity) than other cultivars. The virus incidence of both bulbil and single clover was similar (97 – 100%) while virus titter of OYDV, GCLV, and SLV on bulbil was the lowest than other propagation materials. Detection by RT-PCR from two commercial cultivars showed that ‘Lumbu Hijau’ has less virus incidence than ‘Jawa Lama’. LYSV, OYDV, GCLV were detected on both cultivars but SLV was not found. Further virus indexing using larger number of samples and involving more virus targets needs to be done.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2023-05-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/706</dc:identifier>
	<dc:identifier>10.23960/jhptt.2237-15</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 7-15</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/706/556</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>