Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Hemolysis and hypersensitive tests ease culture collection management of antagonistic bacteria

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 23 No. 2 (2023) · hlm. 24–30

DOI
10.23960/jhptt.22324-30
ISSN
2461-0399, 1411-7525
Terbit
2023-06-19
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

A biosafety assessment is a crucial first step in the management of microbial culture collection to screen and determine unexpected potential plant and human pathogenic bacteria. It is common to collect and store as many fresh culture collections from natural resources before being further evaluated for antagonist bacteria. As a result, a bulk of isolates must be preserved which required more effort and budget. Safety evaluations based on the hemolysis and hypersensitive reactions offer simple tests to ease culture collection management of antagonist bacteria. The study aimed to evaluate the safety of bacterial culture collection for their hemolysis and hypersensitive reactions. Ninety-five isolates of rhizosphere and endophytic bacterial isolates from the culture collections of the Department of Plant Protection-IPB University, Indonesian Industrial and Beverages Crops Research Institute, and Indonesian Spice and Medicinal Crops Research Institute, were evaluated their safety using the hemolysis and hypersensitive tests. The hemolysis test was conducted using blood agar media, from which isolates with a negative (?) reaction were then tested for the hypersensitivity reaction on tobacco leaves. Bacterial isolates passed from both hemolysis and hypersensitivity tests were then preserved by the lyophilization method for long-term storage of culture collection. Based on the hemolysis test, 68 out of 95 bacterial isolates (71.57%) were found to be positive (? or?) reactions. The hypersensitive test showed that 22 of 27 negative hemolysis isolates did not trigger hypersensitivity reactions in tobacco leaves, therefore, they were preserved by lyophilization. The study indicated that a high number of bacterial isolates in the present collection, 68 positive hemolysis, and 5 hypersensitive, need to be re-evaluated due to their safety concerns. The present study highlights the importance of biosafety tests performed in an early stage before the to permanent collection of antagonist isolates.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 92 tautan kepenulisan bersama · 39 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • IPB University
  • Muhammadiyah University of Yogyakarta
  • Universitas Halu Oleo
  • National Research and Innovation Agency
  • State University of Semarang
  • Universitas Airlangga
  • Andalas University
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
GiyantoNational Research and Innovation Agency5
Sri Hendrastuti HidayatIPB University4
Asmar HasanUniversitas Halu Oleo2
Widodo WidodoIPB University2
Haliatur RahmaAndalas University2
Abdjad Asih NawangsihIPB University2
Miftakhurohmah MiftakhurohmahNational Research and Innovation Agency2
Muhammad TaufikUniversitas Halu Oleo1
Siti AisyahIPB University1
Widodo WAfiliasi tidak diketahui1
Muhamad BurhanudinState University of Semarang1
Yusro Edy NugrohoState University of Semarang1
Siti Rohmah SoekarbaUniversity of Indonesia1
Aris Slamet WidodoMuhammadiyah University of Yogyakarta1
Arsy Nur FadilahMuhammadiyah University of Yogyakarta1
Lestari RahayuMuhammadiyah University of Yogyakarta1
Diah Rina KamardianiMuhammadiyah University of Yogyakarta1
Siti Yusi RusimahAfiliasi tidak diketahui1
Novita ChoirunnisaAfiliasi tidak diketahui1
Nur Azizatur RahmawatiMuhammadiyah University of Yogyakarta1
Oki WijayaMuhammadiyah University of Yogyakarta1
Riskhi LathifahMuhammadiyah University of Yogyakarta1
Cahyo Wisnu RubiyantoGifu University1
Muhammad TaufikUniversitas Halu Oleo1
Kikin H. MutaqienAfiliasi tidak diketahui1
TRIKOESOEMANINGTYAS TRIKOESOEMANINGTYASIPB University1
Giyanto GiyantoIPB University1
Christoffol LeiwakabessyIPB University1
Sari NurulitaIPB University1
John E. ThomasIPB University1
Agung SosiawanUniversitas Airlangga1
Darmawan SetijantoUniversitas Airlangga1
Andi KhaeruniUniversitas Halu Oleo1
Budi TjahjonoIPB University1
Antonius SuwantoIPB University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Hemolysis and hypersensitive tests ease culture collection management of antagonistic bacteria</dc:title>
	<dc:creator xml:lang="en">Amaria, Widi</dc:creator>
	<dc:creator xml:lang="en">Sinaga, Meity Suradji</dc:creator>
	<dc:creator xml:lang="en">Mutaqin, Kikin Hamzah</dc:creator>
	<dc:creator xml:lang="en">Supriadi, Supriadi</dc:creator>
	<dc:creator xml:lang="en">Widodo, Widodo</dc:creator>
	<dc:subject xml:lang="en">antagonist bacteria</dc:subject>
	<dc:subject xml:lang="en">biosafety</dc:subject>
	<dc:subject xml:lang="en">early stage</dc:subject>
	<dc:subject xml:lang="en">lyophilization</dc:subject>
	<dc:description xml:lang="en">A biosafety assessment is a crucial first step in the management of microbial culture collection to screen and determine unexpected potential plant and human pathogenic bacteria. It is common to collect and store as many fresh culture collections from natural resources before being further evaluated for antagonist bacteria. As a result, a bulk of isolates must be preserved which required more effort and budget. Safety evaluations based on the hemolysis and hypersensitive reactions offer simple tests to ease culture collection management of antagonist bacteria. The study aimed to evaluate the safety of bacterial culture collection for their hemolysis and hypersensitive reactions. Ninety-five isolates of rhizosphere and endophytic bacterial isolates from the culture collections of the Department of Plant Protection-IPB University, Indonesian Industrial and Beverages Crops Research Institute, and Indonesian Spice and Medicinal Crops Research Institute, were evaluated their safety using the hemolysis and hypersensitive tests. The hemolysis test was conducted using blood agar media, from which isolates with a negative (?) reaction were then tested for the hypersensitivity reaction on tobacco leaves. Bacterial isolates passed from both hemolysis and hypersensitivity tests were then preserved by the lyophilization method for long-term storage of culture collection. Based on the hemolysis test, 68 out of 95 bacterial isolates (71.57%) were found to be positive (? or ?) reactions. The hypersensitive test showed that 22 of 27 negative hemolysis isolates did not trigger hypersensitivity reactions in tobacco leaves, therefore, they were preserved by lyophilization. The study indicated that a high number of bacterial isolates in the present collection, 68 positive hemolysis, and 5 hypersensitive, need to be re-evaluated due to their safety concerns. The present study highlights the importance of biosafety tests performed in an early stage before the to permanent collection of antagonist isolates.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2023-06-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/710</dc:identifier>
	<dc:identifier>10.23960/jhptt.22324-30</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 24-30</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/710/560</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>