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Unveiling alliinase gene candidates in shallots using resistance gene motif-based degenerate primers

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 24 No. 2 (2024) · hlm. 274–283

DOI
10.23960/jhptt.224274-283
ISSN
2461-0399, 1411-7525
Terbit
2024-05-09
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Shallots are integral to Indonesian daily life, with annual production reaching two million tons. However, the superior varieties are often susceptible to disease, highlighting the need for new resistant varieties. Understanding the genetic basis of disease resistance is crucial for breeding efforts aimed at developing new varieties. Alliinase, an enzyme crucial for garlic defense, is a potential candidate for enhancing shallot resistance. This study aims to identify alliinase gene candidates in shallots using degenerate primers. Genomic DNA from the Bima Brebes genotype was isolated, and degenerate primers successfully amplified 600–800 bp fragments. Three sequences were selected for further analysis, with one sequence showing high similarity to known resistance genes. Multiple sequence alignment revealed characteristic resistance gene motifs, supporting their candidacy as resistance genes. Phylogenetic analysis grouped these sequences with known resistance genes, further supporting their potential. These findings provide valuable insights for breeding programs aiming to enhance resistance in shallots through a molecular breeding approach. The identified alliinase gene candidates can be used to develop disease-resistant shallot varieties. Incorporating these genes into breeding programs can enhance resistance, improving yield and stability in shallot production.

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  • Indonesia University of Education
  • National Research and Innovation Agency
  • IPB University
  • Soegijapranata Catholic University
  • Diponegoro University
  • State University of Semarang
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Rekan penulisOrganisasiArtikel bersama
Dheny PrasetyaAfiliasi tidak diketahui2
Henna Rya SunokoDiponegoro University1
Budi WidianarkoSoegijapranata Catholic University1
Novi Ratna DewiState University of Semarang1
Fitri RahmawatiIndonesia University of Education1
Riandi RiandiIndonesia University of Education1
Diana RochintaniawatiIndonesia University of Education1
Ari WidodoIndonesia University of Education1
Tiur Sudiaty SilitongaAfiliasi tidak diketahui1
Fatimah FatimahNational Research and Innovation Agency1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Unveiling alliinase gene candidates in shallots using resistance gene motif-based degenerate primers</dc:title>
	<dc:creator xml:lang="en">Herlina, Lina</dc:creator>
	<dc:creator xml:lang="en">Istiaji, Bonjok</dc:creator>
	<dc:subject xml:lang="en">alliinase</dc:subject>
	<dc:subject xml:lang="en">degenerate primer</dc:subject>
	<dc:subject xml:lang="en">defense mechanism</dc:subject>
	<dc:subject xml:lang="en">molecular marker </dc:subject>
	<dc:description xml:lang="en">Shallots are integral to Indonesian daily life, with annual production reaching two million tons. However, the superior varieties are often susceptible to disease, highlighting the need for new resistant varieties. Understanding the genetic basis of disease resistance is crucial for breeding efforts aimed at developing new varieties. Alliinase, an enzyme crucial for garlic defense, is a potential candidate for enhancing shallot resistance. This study aims to identify alliinase gene candidates in shallots using degenerate primers. Genomic DNA from the Bima Brebes genotype was isolated, and degenerate primers successfully amplified 600–800 bp fragments. Three sequences were selected for further analysis, with one sequence showing high similarity to known resistance genes. Multiple sequence alignment revealed characteristic resistance gene motifs, supporting their candidacy as resistance genes. Phylogenetic analysis grouped these sequences with known resistance genes, further supporting their potential. These findings provide valuable insights for breeding programs aiming to enhance resistance in shallots through a molecular breeding approach. The identified alliinase gene candidates can be used to develop disease-resistant shallot varieties. Incorporating these genes into breeding programs can enhance resistance, improving yield and stability in shallot production.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2024-05-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/800</dc:identifier>
	<dc:identifier>10.23960/jhptt.224274-283</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 274-283</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/800/632</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>