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Molecular characterization and effectiveness cross-protection of weak strains against super-infection malignant strains Cucumber mosaic virus (CMV) on cayenne pepper (Capsicum annuum L.) in Lampung, Indonesia

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 25 No. 1 (2025) · hlm. 63–75

DOI
10.23960/jhptt.12563-75
ISSN
2461-0399, 1411-7525
Terbit
2025-02-22
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Cayenne pepper (Capsicum annuum L.) is a horticultural crop of significant economic importance. One of the major threats to its cultivation is the Cucumber mosaic virus (CMV), which can cause severe yield losses and crop failures. This study aimed to isolate weak CMV strains from natural populations of cayenne pepper, characterize their molecular properties, and evaluate the effectiveness of cross-protection conferred by weak strains against superinfection by virulent CMV strains. The research was conducted in three stages: virus isolation, molecular characterization, and evaluation of cross-protection effectiveness. Data analysis was performed using the MEGA v.11.0.11 software and analysis of variance (ANOVA). The findings identified the C1 isolate as a highly virulent CMV strain. Molecular analysis of naturally infected samples confirmed the presence of CMV through the amplification of specific DNA bands. Inoculation tests revealed distinct differences between weak and malignant strains, particularly in symptom severity and molecular characteristics, such as genetic distance. Furthermore, the weak strains demonstrated a protective effect, significantly reducing the infection rate of virulent CMV strains. This was evidenced by variations in symptom expression, disease severity, plant resistance, chlorophyll levels, carbohydrate content, and peroxidase enzyme activity. These findings highlight the potential application of weak CMV strains in developing disease management strategies for cayenne pepper cultivation.

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  • Lampung University
  • State University of Semarang
  • Institut Teknologi Sepuluh Nopember
  • Universitas Halu Oleo
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Rike Puspitasari TaminAfiliasi tidak diketahui3
Riana AnggrainiAfiliasi tidak diketahui2
Fazriyas FazriyasAfiliasi tidak diketahui2
Joko PrasetyoInstitut Teknologi Sepuluh Nopember2
Lestari WibowoAfiliasi tidak diketahui2
Radix SuharjoAfiliasi tidak diketahui2
Desma AnggrainiAfiliasi tidak diketahui2
Mohammad HoesainAfiliasi tidak diketahui2
Fariz Kustiawan AlfarisyAfiliasi tidak diketahui2
Ankardiansyah Pandu PradanaAfiliasi tidak diketahui2
Asrul SaniUniversitas Halu Oleo1
Muhammad SudiaAfiliasi tidak diketahui1
Emantis RosaLampung University1
Supiyanto SupiyantoLampung University1
Nismah NukmalLampung University1
Nabela H.N. HarfianiLampung University1
Mahfut MahfutLampung University1
Endang NurcahyaniLampung University1
Akhmad NurdinAfiliasi tidak diketahui1
Iqbal ZulfikarAfiliasi tidak diketahui1
Angger Bagus PrasetiyoAfiliasi tidak diketahui1
Ahmad SofwanAfiliasi tidak diketahui1
Eka HaryaniAfiliasi tidak diketahui1
Ihsan Nuril HikamAfiliasi tidak diketahui1
Asep SutarmanAfiliasi tidak diketahui1
Zaharuddin ZaharuddinAfiliasi tidak diketahui1
Thriwaty ArsalAfiliasi tidak diketahui1
Martitah MartitahAfiliasi tidak diketahui1
Ani RusilowatiState University of Semarang1
Nathan HindartoState University of Semarang1
Eva AchmadAfiliasi tidak diketahui1
Asrizal PaimanAfiliasi tidak diketahui1
Marwoto MarwotoAfiliasi tidak diketahui1
Hamzah HamzahAfiliasi tidak diketahui1
Maria UlfaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Molecular characterization and effectiveness cross-protection of weak strains against super-infection malignant strains Cucumber mosaic virus (CMV) on cayenne pepper (Capsicum annuum L.) in Lampung, Indonesia</dc:title>
	<dc:creator xml:lang="en">Mahfut, Mahfut</dc:creator>
	<dc:creator xml:lang="en">Sari, Mai</dc:creator>
	<dc:creator xml:lang="en">Akin, Hasriadi Mat</dc:creator>
	<dc:creator xml:lang="en">Wahyuningsih, Sri</dc:creator>
	<dc:creator xml:lang="en">Irawan, Bambang</dc:creator>
	<dc:subject xml:lang="en">cayenne pepper</dc:subject>
	<dc:subject xml:lang="en">cross-protection</dc:subject>
	<dc:subject xml:lang="en">Cucumber mosaic virus (CMV)</dc:subject>
	<dc:subject xml:lang="en">molecular characterization</dc:subject>
	<dc:subject xml:lang="en">weak strains</dc:subject>
	<dc:description xml:lang="en">Cayenne pepper (Capsicum annuum L.) is a horticultural crop of significant economic importance. One of the major threats to its cultivation is the Cucumber mosaic virus (CMV), which can cause severe yield losses and crop failures. This study aimed to isolate weak CMV strains from natural populations of cayenne pepper, characterize their molecular properties, and evaluate the effectiveness of cross-protection conferred by weak strains against superinfection by virulent CMV strains. The research was conducted in three stages: virus isolation, molecular characterization, and evaluation of cross-protection effectiveness. Data analysis was performed using the MEGA v.11.0.11 software and analysis of variance (ANOVA). The findings identified the C1 isolate as a highly virulent CMV strain. Molecular analysis of naturally infected samples confirmed the presence of CMV through the amplification of specific DNA bands. Inoculation tests revealed distinct differences between weak and malignant strains, particularly in symptom severity and molecular characteristics, such as genetic distance. Furthermore, the weak strains demonstrated a protective effect, significantly reducing the infection rate of virulent CMV strains. This was evidenced by variations in symptom expression, disease severity, plant resistance, chlorophyll levels, carbohydrate content, and peroxidase enzyme activity. These findings highlight the potential application of weak CMV strains in developing disease management strategies for cayenne pepper cultivation.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2025-02-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/815</dc:identifier>
	<dc:identifier>10.23960/jhptt.12563-75</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 63-75</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/815/643</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>