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Effectiveness of entomopathogenic fungal combinations on eggs and larval development of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in corn plants

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 26 No. 2 (2026) · hlm. 290–303

DOI
10.23960/jhptt.226290-303
ISSN
2461-0399, 1411-7525
Terbit
2026-04-23
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Spodoptera frugiperda J.E. Smith is an invasive pest of corn, requiring effective and environmentally friendly control strategies. This study aimed to evaluate the effectiveness of three entomopathogenic fungal (EPF) isolates, namely Beauveria bassiana WS, Metarhizium anisopliae B22C, and Trichoderma asperellum A116, tested as single isolates or in combination against S. frugiperda eggs and larvae. The experiment used a Completely Randomized Design (CRD) with eight treatments (three single treatments, four combinations, and a control) and four replications. Conidial suspensions were applied by spraying onto eggs and second-instar larvae. Parameters observed included the percentage of unhatched eggs, larval mortality, and pupal and adult development. Chitinase, protease, and lipase activities were qualitatively tested to support the identification of pathogenicity mechanisms. Data were analyzed using analysis of variance (ANOVA), followed by the LSD test at a 5% significance level. The results showed that EPF combinations increased mortality and inhibited the development of S. frugiperda. The combination of B. bassiana WS + T. asperellum A116 was the most effective treatment, inhibiting egg hatching by 85% and reducing pupal and adult formation to 25.95% and 12.96%, respectively. The highest mortality of first-instar larvae was observed in the single treatment of B. bassiana WS and the tertiary combination of B. bassiana WS + M. anisopliae B22C + T. asperellum A116, at 24.18% and 22.91%, respectively. Consistent enzymatic activity indicated potential biocontrol synergy through the production of cuticle-degrading enzymes. This combination is recommended for field-scale testing as an environmentally friendly alternative to reduce dependence on chemical insecticides.

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  • National Research and Innovation Agency
  • Andalas University
  • IPB University
  • University of Bengkulu
  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • King Mongkut's Institute of Technology Ladkrabang
  • National University of Battambang
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Rekan penulisOrganisasiArtikel bersama
GiyantoNational Research and Innovation Agency2
Meity Suradji SinagaIPB University2
Aprizal ZainalAndalas University2
Fauzan Abid Hanif NasutionAndalas University2
Zurai RestiAndalas University2
Siska Chiko EfendiAndalas University1
Syahbanuari SitompulAfiliasi tidak diketahui1
I Nyoman WidiartaNational Research and Innovation Agency1
Yusmani PrayogoNational Research and Innovation Agency1
Sempurna Br. GintingUniversity of Bengkulu1
Marida Santi Yudha Ika BayuNational Research and Innovation Agency1
Yuliantoro BaliadiNational Research and Innovation Agency1
Didik HarnowoNational Research and Innovation Agency1
Sri Wahyuni IndiatiNational Research and Innovation Agency1
Gatut Wahyu Anggoro SusantoNational Research and Innovation Agency1
Yadi SuryadiIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development1
Made Jana MejayaNational Research and Innovation Agency1
Sernan Entierro FortusKing Mongkut's Institute of Technology Ladkrabang1
Vibol PeuoNational University of Battambang1
Memen SurahmanIPB University1
Suryati .Afiliasi tidak diketahui1
Memen SuharmanIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Effectiveness of entomopathogenic fungal combinations on eggs and larval development of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in corn plants</dc:title>
	<dc:creator xml:lang="en">Vajri, Indri Yanil</dc:creator>
	<dc:creator xml:lang="en">Trizelia, Trizelia</dc:creator>
	<dc:creator xml:lang="en">Rahma, Haliatur  </dc:creator>
	<dc:creator xml:lang="en">Yusniwati, Yusniwati</dc:creator>
	<dc:subject xml:lang="en">Biological control</dc:subject>
	<dc:subject xml:lang="en">combination</dc:subject>
	<dc:subject xml:lang="en">entomopathogenic fungi</dc:subject>
	<dc:subject xml:lang="en">ovicides</dc:subject>
	<dc:subject xml:lang="en">Spodoptera frugiperda</dc:subject>
	<dc:description xml:lang="en">Spodoptera frugiperda J.E. Smith is an invasive pest of corn, requiring effective and environmentally friendly control strategies. This study aimed to evaluate the effectiveness of three entomopathogenic fungal (EPF) isolates, namely Beauveria bassiana WS, Metarhizium anisopliae B22C, and Trichoderma asperellum A116, tested as single isolates or in combination against S. frugiperda eggs and larvae. The experiment used a Completely Randomized Design (CRD) with eight treatments (three single treatments, four combinations, and a control) and four replications. Conidial suspensions were applied by spraying onto eggs and second-instar larvae. Parameters observed included the percentage of unhatched eggs, larval mortality, and pupal and adult development. Chitinase, protease, and lipase activities were qualitatively tested to support the identification of pathogenicity mechanisms. Data were analyzed using analysis of variance (ANOVA), followed by the LSD test at a 5% significance level. The results showed that EPF combinations increased mortality and inhibited the development of S. frugiperda. The combination of B. bassiana WS + T. asperellum A116 was the most effective treatment, inhibiting egg hatching by 85% and reducing pupal and adult formation to 25.95% and 12.96%, respectively. The highest mortality of first-instar larvae was observed in the single treatment of B. bassiana WS and the tertiary combination of B. bassiana WS + M. anisopliae B22C + T. asperellum A116, at 24.18% and 22.91%, respectively. Consistent enzymatic activity indicated potential biocontrol synergy through the production of cuticle-degrading enzymes. This combination is recommended for field-scale testing as an environmentally friendly alternative to reduce dependence on chemical insecticides.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2026-04-23</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/993</dc:identifier>
	<dc:identifier>10.23960/jhptt.226290-303</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 26 No. 2 (2026): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 290-303</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/993/705</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>