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Enhanced nematicidal potential of MnCl2-fortified Bacillus sp. cell-free supernatant against Meloidogyne incognita

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 26 No. 2 (2026) · hlm. 327–341

DOI
10.23960/jhptt.226327-341
ISSN
2461-0399, 1411-7525
Terbit
2026-05-06
Penerbit
Universitas Lampung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Meloidogyne incognita causes major yield losses, underscoring the need for sustainable control strategies. This study is the first to demonstrate that micronutrient fortification and pH modulation of Bacillus sp. SK07 cultures enhance the nematicidal activity of their cell-free supernatant (CFS) and alter metabolite composition. Bacillus sp. SK07 was cultured in Nutrient Broth supplemented with MnCl2 at 0, 50, or 75 ppm, with pH adjusted to 6 or 8. A 10% (v/v) CFS was evaluated in vitro against eggs and second-stage juveniles (J2) over 7 days using a completely randomized design with five treatments and five replicates. The most effective treatment—50 ppm MnCl2 at pH 8—resulted in 96.4% egg-hatch inhibition and 63.6% J2 mortality at 168 hours, significantly higher than the unfortified control (91.8% and 49.4%, respectively). Chemical analysis revealed substantial changes in metabolite composition: unfortified cultures exhibited 9 peaks, whereas the optimized treatment produced 27 peaks, indicating increased chemical diversity. Several bioactive compounds, including fatty acid derivatives and aromatic esters, were identified. These compounds belong to diverse chemical classes such as acids, esters, alcohols, and hydrocarbons, many of which are known for their nematicidal activity. These findings demonstrate that MnCl2 fortification at pH 8 effectively enhanced the bioactivity of Bacillus sp. SK07, offering a promising and sustainable approach for managing M. incognita.

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  • Universitas Jember
  • Universitas Nurul Jadid
  • Universitas Borneo Tarakan
  • Sultan Zainal Abidin University
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Rekan penulisOrganisasiArtikel bersama
Fariz Kustiawan AlfarisyAfiliasi tidak diketahui4
Mohammad HoesainAfiliasi tidak diketahui3
Muh. AdiwenaUniversitas Borneo Tarakan3
Sri WahyuningsihAfiliasi tidak diketahui2
Abdullah AbdullahUniversitas Nurul Jadid1
Rahimah EmbongSultan Zainal Abidin University1
Afri PrayudiUniversitas Jember1
Evita Soliha HaniUniversitas Jember1
Alfina Uswatun HasanahUniversitas Jember1
Luh Putu SuciatiUniversitas Jember1
Rachmi MasnilahAfiliasi tidak diketahui1
Suhartiningsih Dwi NurchayantiAfiliasi tidak diketahui1
Dwi Tirta SariAfiliasi tidak diketahui1
Farchan Mushal Al RamadhanAfiliasi tidak diketahui1
RismayaniAfiliasi tidak diketahui1
Sri Ita TariganAfiliasi tidak diketahui1
Toyoshi YoshigaAfiliasi tidak diketahui1
Amam AmamUniversitas Jember1
Pradiptya Ayu HarsitaAfiliasi tidak diketahui1
Diana PutriAfiliasi tidak diketahui1
Deril Indana DamayantiAfiliasi tidak diketahui1
Ahmed Ibrahim Alrashid YousifAfiliasi tidak diketahui1
Mohammad RondhiAfiliasi tidak diketahui1
Rokhani RokhaniAfiliasi tidak diketahui1
Ad Hariyanto AdiAfiliasi tidak diketahui1
Agus SuprionoAfiliasi tidak diketahui1
Triana Dewi HapsariAfiliasi tidak diketahui1
Ahmad Fatikhul KhasanAfiliasi tidak diketahui1
Diana FauziyahAfiliasi tidak diketahui1
Sugeng RahartoAfiliasi tidak diketahui1
Eko SugihartoAfiliasi tidak diketahui1
Adi Heru HusodoAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Enhanced nematicidal potential of MnCl2-fortified Bacillus sp. cell-free supernatant against Meloidogyne incognita</dc:title>
	<dc:creator xml:lang="en">Anggraini, Dyah Retno</dc:creator>
	<dc:creator xml:lang="en">Savitri, Dyah Ayu</dc:creator>
	<dc:creator xml:lang="en">Jadmiko, Mochammad Wildan</dc:creator>
	<dc:creator xml:lang="en">Sofia, Sofia</dc:creator>
	<dc:creator xml:lang="en">Sudarko, Sudarko</dc:creator>
	<dc:creator xml:lang="en">Hariyati, Yuli</dc:creator>
	<dc:creator xml:lang="en">Nurlaelita, Khaerani</dc:creator>
	<dc:creator xml:lang="en">Pradana, Ankardiansyah Pandu</dc:creator>
	<dc:subject xml:lang="en">Bioassay</dc:subject>
	<dc:subject xml:lang="en">GC–MS</dc:subject>
	<dc:subject xml:lang="en">hatch inhibition</dc:subject>
	<dc:subject xml:lang="en">root-knot nematode</dc:subject>
	<dc:subject xml:lang="en">secondary metabolites</dc:subject>
	<dc:description xml:lang="en">Meloidogyne incognita causes major yield losses, underscoring the need for sustainable control strategies. This study is the first to demonstrate that micronutrient fortification and pH modulation of Bacillus sp. SK07 cultures enhance the nematicidal activity of their cell-free supernatant (CFS) and alter metabolite composition. Bacillus sp. SK07 was cultured in Nutrient Broth supplemented with MnCl2 at 0, 50, or 75 ppm, with pH adjusted to 6 or 8. A 10% (v/v) CFS was evaluated in vitro against eggs and second-stage juveniles (J2) over 7 days using a completely randomized design with five treatments and five replicates. The most effective treatment—50 ppm MnCl2 at pH 8—resulted in 96.4% egg-hatch inhibition and 63.6% J2 mortality at 168 hours, significantly higher than the unfortified control (91.8% and 49.4%, respectively). Chemical analysis revealed substantial changes in metabolite composition: unfortified cultures exhibited 9 peaks, whereas the optimized treatment produced 27 peaks, indicating increased chemical diversity. Several bioactive compounds, including fatty acid derivatives and aromatic esters, were identified. These compounds belong to diverse chemical classes such as acids, esters, alcohols, and hydrocarbons, many of which are known for their nematicidal activity. These findings demonstrate that MnCl2 fortification at pH 8 effectively enhanced the bioactivity of Bacillus sp. SK07, offering a promising and sustainable approach for managing M. incognita.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2026-05-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/1003</dc:identifier>
	<dc:identifier>10.23960/jhptt.226327-341</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 26 No. 2 (2026): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 327-341</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/1003/710</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>