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UTILIZATION OF cpDNA SEQUENCES TO IDENTIFY 15 MANGO ACCESSIONS

AGRIVITA, Journal of Agricultural Science · Vol. 38 No. 2 (2016) · hlm. 155–162

DOI
10.17503/agrivita.v38i2.767
ISSN
2477-8516, 0126-0537
Terbit
2016-04-27
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0/

Abstrak

Information about genetic relationship and genetic diversity are an inevitable need in mango breeding program. Base substitution value, genetic distance and grouping of 15 mango accessions based on accessions in chloroplast DNA (cpDNA) among 15 mango accessions were assessed. The samples were originated from Mango Germplasm, Cukur Gondang Research Station of Indonesian Tropical Fruits Research Institute, Pasuruan, East Java. Sequencing of Chloroplast DNA was used to obtain nucleotide sequence data. Paired specific primer rpl20 F - rps12 R and atp F - rbcL R were used for amplification of non-coding area of mango DNA chloroplast and sequencing processes as well. All data were analyzed using Software MEGA 6. The result showed that total numbers and nucleotide base sequences varied among all accessions. All accessions were grouped in five different clusters that might be used as source of parental breeding.

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  • Universitas Gadjah Mada
  • IPB University
  • Muhammadiyah University of Surakarta
  • Cenderawasih University
  • Universitas Bangun Nusantara
  • Institut Teknologi Sepuluh Nopember
  • Universitas Jember
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Rekan penulisOrganisasiArtikel bersama
Rani Agustina WulandariUniversitas Gadjah Mada3
K. KristamtiniAfiliasi tidak diketahui3
Kristamtini KristamtiniNational Research and Innovation Agency3
Enik Nurlaili AfifahUniversitas Gadjah Mada2
Sugiharto Budi SantosoMuhammadiyah University of Surakarta2
Paramita CahyaningrumUniversitas Gadjah Mada2
Ratna Dewi EskundariUniversitas Bangun Nusantara2
Didik IndradewaUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Yunus Pajanjan PaulanganCenderawasih University1
Muh. Arsyad Al. AminIPB University1
Barnabas BarapadangCenderawasih University1
Yudi WahyudinIPB University1
Mahfud MahfudInstitut Teknologi Sepuluh Nopember1
Dimas Tri AsmaraUniversitas Gadjah Mada1
Arman WijonarkoUniversitas Gadjah Mada1
Vega Kartika SariUniversitas Jember1
Vina Eka AristyaUniversitas Gadjah Mada1
Fitri HandayaniAfiliasi tidak diketahui1
Eko HanudinUniversitas Gadjah Mada1
Dewi Ratna NurhayatiUniversitas Slamet Riyadi1
Prapto YudonoUniversitas Gadjah Mada1
Yuli PriyanaMuhammadiyah University of Surakarta1
Suyadi SuyadiUniversity of Brawijaya1
Titin SetyoriniAfiliasi tidak diketahui1
Sapto IndriokoUniversitas Gadjah Mada1
A. RimbawantoAfiliasi tidak diketahui1
Yudi Agus SetiawanIPB University1
Widiatmaka WidiatmakaIPB University1
Wiwin AmbarwulanIPB University1
Hefni EffendiIPB University1
Muhamad Yanuar Jarwadi PurwantoIPB University1
. SUPUTAAfiliasi tidak diketahui1
Gesang PratyadhiraksanaUniversitas Gadjah Mada1
Tri Murniningtyas Puji LestariAfiliasi tidak diketahui1
Elisa AnggraeniIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">UTILIZATION OF cpDNA SEQUENCES TO IDENTIFY 15 MANGO ACCESSIONS</dc:title>
	<dc:creator>Sukartini, Sukartini</dc:creator>
	<dc:creator>Taryono, Taryono</dc:creator>
	<dc:creator>Basunanda, Panjisakti</dc:creator>
	<dc:creator>Murti, Rudi Hari</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">cpDNA; genetic relationships; mango; sequencing; variability</dc:subject>
	<dc:description xml:lang="en-US">Information about genetic relationship and genetic diversity are an inevitable need in mango breeding program. Base substitution value, genetic distance and grouping of 15 mango accessions based on accessions in chloroplast DNA (cpDNA) among 15 mango accessions were assessed. The samples were originated from Mango Germplasm, Cukur Gondang Research Station of Indonesian Tropical Fruits Research Institute, Pasuruan, East Java. Sequencing of Chloroplast DNA was used to obtain nucleotide sequence data. Paired specific primer rpl20 F - rps12 R and atp F - rbcL R were used for amplification of non-coding area of mango DNA chloroplast and sequencing processes as well. All data were analyzed using Software MEGA 6. The result showed that total numbers and nucleotide base sequences varied among all accessions. All accessions were grouped in five different clusters that might be used as source of parental breeding.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-04-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/767</dc:identifier>
	<dc:identifier>10.17503/agrivita.v38i2.767</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 38, No 2 (2016): JUNE; 155-162</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v38i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/767/706</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 AGRIVITA Journal of Agricultural Science</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0/</dc:rights>
</oai_dc:dc>