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Diversity of Culturable Bacterial Gut Microbiome of Oryctes rhinoceros Larvae and Their Potency as Source of Protease and Lipase Enzymes

AGRIVITA, Journal of Agricultural Science · Vol. 45 No. 3 (2023) · hlm. 580–588

DOI
10.17503/agrivita.v45i3.4316
ISSN
2477-8516, 0126-0537
Terbit
2023-10-31
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Oryctes rhinoceros is an important pest of various coconut and palm species. Studies on the bacterial gut microbiome of O. rhinoceros larvae and their potential use as producers of industrial enzymes are still limited. This study aims to examine the diversity and abundance of culturable bacterial microbiome in the gut of O. rhinoceros and their potential to produce protease and lipase. This study consisted of several stages, i.e., collection of O. rhinoceros larvae from coconut plantations; isolation of culturable bacteria from larval gut; protease and lipase enzyme activity assays; and partial characterization of culturable gut bacteria in terms of morphology, physiology, and biochemistry. There were seven bacteria that were isolated from the gut of O. rhinoceros, which were differentiated by their morphotype. The seven bacterial isolates also showed variations in physiological, and biochemical characteristics, and the larval guts were dominated by Gram-positive bacteria. The Shannon diversity index was 1.33, evenness was 0.682, richness was 7, and the abundance of each type of bacteria varied between 2x105 and 9.1x1010. Bacteria UBOG1, UBOG3, and UBOG5 were able to produce protease, but all seven bacteria were unable to produce lipase. Thus, bacterial isolates UBOG1, UBOG3, and UBOG5 are potential to be used in industry.

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  • University of Brawijaya
  • Hasanuddin University
  • National Research and Innovation Agency
  • Philippine Rice Research Institute
  • Padjadjaran University
  • Universitas Muhammadiyah Malang
  • The University of Osaka
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Abdul Latief AbadiUniversity of Brawijaya5
Muhammad Akhid Syib’liUniversity of Brawijaya5
Muhammad Bayu MarioHasanuddin University4
Hagus TarnoUniversity of Brawijaya4
Muh. Ridha Taqwa TangHasanuddin University3
Aminudin AfandhiUniversity of Brawijaya3
Retno Dyah PuspitariniUniversity of Brawijaya3
Eriyanto YusnawanNational Research and Innovation Agency3
Rina RachmawatiUniversity of Brawijaya3
Bayu Aji PamungkasUniversity of Brawijaya3
Wita FirdausiUniversity of Brawijaya2
Liliek SulistyowatiUniversity of Brawijaya2
Adi SetiawanUniversity of Brawijaya2
Nurul AiniUniversity of Brawijaya2
Wiwin Sumiya Dwi YamikaUniversity of Brawijaya2
Ludji Pantja AstutiUniversity of Brawijaya2
Lekhnath KafleAfiliasi tidak diketahui2
Jue-Liang HsuAfiliasi tidak diketahui2
Ardiyan TarunaUniversity of Brawijaya2
Rifani Rusiana DewiUniversity of Brawijaya2
Syarifah Maulidya RahmahUniversity of Brawijaya2
Anisya Putri KarinaUniversity of Brawijaya2
Muhammad Fariq Al HusniUniversity of Brawijaya2
Bayu Aji PamungkasUniversity of Brawijaya2
Sastia Prama PutriThe University of Osaka2
Wardani SyahraniHasanuddin University1
Rahmat RahmatHasanuddin University1
M. TakwaHasanuddin University1
Athiyyah Salsabilla LalisuHasanuddin University1
A. M. Bintang Ramadhan GaligoHasanuddin University1
Ravindra Chandra JoshiPhilippine Rice Research Institute1
Ahwiyah Ekawaty SaidHasanuddin University1
Yani MaharaniPadjadjaran University1
Erfan Dani SeptiaUniversitas Muhammadiyah Malang1
Alfi InayatiUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Diversity of Culturable Bacterial Gut Microbiome of Oryctes rhinoceros Larvae and Their Potency as Source of Protease and Lipase Enzymes</dc:title>
	<dc:creator>Aini, Luqman Qurata</dc:creator>
	<dc:creator>Dewi, Fibrianti Shinta</dc:creator>
	<dc:creator>Trianti, Irisa</dc:creator>
	<dc:creator>Fernando, Ito</dc:creator>
	<dc:creator>Setiawan, Yogo</dc:creator>
	<dc:subject xml:lang="en-US">disease-plant science</dc:subject>
	<dc:subject xml:lang="en-US">Abundance; Diversity; Industrial enzymes; Lipase; Protease</dc:subject>
	<dc:description xml:lang="en-US">Oryctes rhinoceros is an important pest of various coconut and palm species. Studies on the bacterial gut microbiome of O. rhinoceros larvae and their potential use as producers of industrial enzymes are still limited. This study aims to examine the diversity and abundance of culturable bacterial microbiome in the gut of O. rhinoceros and their potential to produce protease and lipase. This study consisted of several stages, i.e., collection of O. rhinoceros larvae from coconut plantations; isolation of culturable bacteria from larval gut; protease and lipase enzyme activity assays; and partial characterization of culturable gut bacteria in terms of morphology, physiology, and biochemistry. There were seven bacteria that were isolated from the gut of O. rhinoceros, which were differentiated by their morphotype. The seven bacterial isolates also showed variations in physiological, and biochemical characteristics, and the larval guts were dominated by Gram-positive bacteria. The Shannon diversity index was 1.33, evenness was 0.682, richness was 7, and the abundance of each type of bacteria varied between 2x105 and 9.1x1010. Bacteria UBOG1, UBOG3, and UBOG5 were able to produce protease, but all seven bacteria were unable to produce lipase. Thus, bacterial isolates UBOG1, UBOG3, and UBOG5 are potential to be used in industry.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2023-10-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4316</dc:identifier>
	<dc:identifier>10.17503/agrivita.v45i3.4316</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 45, No 3 (2023); 580-588</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v45i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4316/1652</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4316/3004</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>