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Spermatozoa Quality of Arab Chickens Using Different Combinations of Physiological Saline and Egg Yolk Extenders

JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) · Vol. 13 No. 1 (2026) · pp. 1–6

ISSN
2406-9337, 2406-7849
Published
2026-01-30
Publisher
Faculty of Animal Science, Universitas Halu Oleo
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Arab chickens are a promising poultry breed due to their adaptability, disease resistance, and production potential. Enhancing reproductive efficiency through artificial insemination requires semen extenders capable of preserving spermatozoa viability during storage. This study evaluated the quality of Arab chicken spermatozoa diluted with different combinations of physiological saline and egg yolk extenders during short-term cold storage. Fresh semen was collected from three Arab chicken roosters aged 1–1.5 years and diluted at a ratio of 1:3 using five extender formulations: P0 (100% physiological saline, control), P1 (90% physiological saline + 10% egg yolk), P2 (85% physiological saline + 15% egg yolk), P3 (80% physiological saline + 20% egg yolk), and P4 (75% physiological saline + 25% egg yolk). Diluted semen was stored at 5 °C and evaluated at 0, 6, 12, 18, and 24 hours. Semen quality parameters included individual spermatozoa motility and plasma membrane integrity assessed using the hypo-osmotic swelling (HOS) test. The results showed a progressive decline in motility and plasma membrane integrity with increasing storage duration across all treatments. Statistical analysis indicated no significant effects of extender combinations on spermatozoa motility or plasma membrane integrity during storage, except at 0 hours for plasma membrane integrity. Although egg yolk supplementation up to 25% did not significantly enhance spermatozoa quality, the combination of 75% physiological saline and 25% egg yolk maintained spermatozoa quality comparable to other treatments during short-term cold storage. These findings indicate that physiological saline–egg yolk extenders are suitable for short-term storage of Arab chicken semen in artificial insemination programs.

Keywords

Collaboration network of the authors

34 authors · 85 co-authorship links · 8 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Kanjuruhan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Enike Dwi KusumawatiUniversitas Kanjuruhan4
Dyah Lestari YuliantiUniversitas Kanjuruhan2
Aju Tjatur Nugroho KrisnaningsihUniversitas Kanjuruhan2
Amiluddin IndiUniversitas Halu Oleo1
Muhammad Nur AfandyAffiliation unknown1
La DimanAffiliation unknown1
Sugeng SantosoUniversitas Kanjuruhan1
Harapin HafidUniversitas Halu Oleo1
Yana WatiUniversitas Halu Oleo1
La Ode Ba’aUniversitas Halu Oleo1
Arsiwan ArsiwanUniversitas Halu Oleo1
Takdir SailiUniversitas Halu Oleo1
Selvinus Lawu WoliUniversitas Kanjuruhan1
Waluyo Edi SusantoUniversitas Kanjuruhan1
Ilma Sarimustaqiyma RianseAffiliation unknown1
Wa Kuasa BakaAffiliation unknown1
Usman RianseAffiliation unknown1
Sarty SarabiahAffiliation unknown1
Fendi SudianataUniversitas Kanjuruhan1
Kris Nur UtomoUniversitas Kanjuruhan1
La Ode Arsad SaniUniversitas Halu Oleo1
Danang PrasetyoUniversitas Halu Oleo1
Mansur MansurAffiliation unknown1
Rahman RahmanAffiliation unknown1
A. IndiAffiliation unknown1
Y. YadiAffiliation unknown1
W. KurniawanAffiliation unknown1
La Ode NafiuUniversitas Halu Oleo1
Sulna SulnaAffiliation unknown1
Takdir SailiAffiliation unknown1
Darni DarniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jitro.uho.ac.id/index.php/journal/oai, first harvested 29 Okt 2026, last seen 29 Okt 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Spermatozoa Quality of Arab Chickens Using Different Combinations of Physiological Saline and Egg Yolk Extenders</dc:title>
	<dc:creator>Saniasih, Ni Kadek</dc:creator>
	<dc:creator>Rahman</dc:creator>
	<dc:creator>Rahadi, Syam</dc:creator>
	<dc:subject xml:lang="en-US">Arab chickens</dc:subject>
	<dc:subject xml:lang="en-US">semen extender</dc:subject>
	<dc:subject xml:lang="en-US">egg yolk</dc:subject>
	<dc:subject xml:lang="en-US">spermatozoa quality</dc:subject>
	<dc:subject xml:lang="en-US">artificial insemination</dc:subject>
	<dc:description xml:lang="en-US">Arab chickens are a promising poultry breed due to their adaptability, disease resistance, and production potential. Enhancing reproductive efficiency through artificial insemination requires semen extenders capable of preserving spermatozoa viability during storage. This study evaluated the quality of Arab chicken spermatozoa diluted with different combinations of physiological saline and egg yolk extenders during short-term cold storage. Fresh semen was collected from three Arab chicken roosters aged 1–1.5 years and diluted at a ratio of 1:3 using five extender formulations: P0 (100% physiological saline, control), P1 (90% physiological saline + 10% egg yolk), P2 (85% physiological saline + 15% egg yolk), P3 (80% physiological saline + 20% egg yolk), and P4 (75% physiological saline + 25% egg yolk). Diluted semen was stored at 5 °C and evaluated at 0, 6, 12, 18, and 24 hours. Semen quality parameters included individual spermatozoa motility and plasma membrane integrity assessed using the hypo-osmotic swelling (HOS) test. The results showed a progressive decline in motility and plasma membrane integrity with increasing storage duration across all treatments. Statistical analysis indicated no significant effects of extender combinations on spermatozoa motility or plasma membrane integrity during storage, except at 0 hours for plasma membrane integrity. Although egg yolk supplementation up to 25% did not significantly enhance spermatozoa quality, the combination of 75% physiological saline and 25% egg yolk maintained spermatozoa quality comparable to other treatments during short-term cold storage. These findings indicate that physiological saline–egg yolk extenders are suitable for short-term storage of Arab chicken semen in artificial insemination programs.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Animal Science, Universitas Halu Oleo</dc:publisher>
	<dc:date>2026-01-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jitro.uho.ac.id/index.php/journal/article/view/7</dc:identifier>
	<dc:source xml:lang="en-US">JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis); Vol. 13 No. 1 (2026): JITRO, January; 1-6</dc:source>
	<dc:source>2406-9337</dc:source>
	<dc:source>2406-7849</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jitro.uho.ac.id/index.php/journal/article/view/7/7</dc:relation>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>