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Motilitas Dan Daya Hidup Spermatozoa Asal Epididimis Sapi Persilangan yang Diencerkan dengan Air Tebu

JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) · Vol. 7 No. 1 (2020) · pp. 70–76

DOI
10.33772/jitro.v7i1.8855
ISSN
2406-9337
Published
2020-01-30
Publisher
Universitas Halu Oleo
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

ABSTRAKAir tebu diketahui mengandung sukrosa yang dapat meningkatkan energi bagi motilitas spermatozoa. Penelitian ini bertujuan untuk mengetahui kemampuan air tebu dan kuning telur dalam mempertahankan kualitas spermatozoa asal kauda epididymis sapi persilangan. Penelitian ini menggunakan epididimis sapi persilangan dari rumah pemotongan hewan (RPH) Basirih Kotamadya Banjarmasin. Spermatozoa yang dikoleksi ditempatkan ke dalam empat tabung reaksi masing-masing terdiri dari: 80% pengencer laktosa + 20% kuning telur ayam ras (kontrol), 45% air tebu + 45% akubidestilata + 10% kuning telur ayam ras (ATKT10), 42,5% air tebu + 42,5% akubidestilata + 15% kuning telur ayam ras (ATKT15), dan 40% air tebu + 40% akubidestilata + 20% kuning telur ayam ras (ATKT20). Parameter pengamatan adalah motilitas, daya hidup, dan membran plasma utuh. Hasil penelitian menunjukkan motilitas pengencer kontrol (65%), berbeda nyata (P0,05) dengan ATKT10 (49%), ATKT15 (48%) dan ATKT20 (54%), namun demikian ATKT20 masih lebih tinggi (P0,05) dengan ATKT10 dan ATKT15. Persentase daya hidup spermatozoa berbeda nyata (P0,05) antara kontrol (79,60%) dengan ATKT10 (72,40%), ATKT15 (73,20%), dan ATKT20 (74,60%), akan tetapi ATKT20 dan ATKT15 masih lebih baik (P0,05) dari ATKT10. Untuk persentase MPU, kontrol sebesar 76,4%, sama dengan (P0,05) perlakuan ATKT 20 sebesar 76%, akan tetapi persentase MPU kontrol masih lebih baik (P0,05) dari ATKT 10 sebesar 71,80% dan ATKT15 sebesar 74,20%. Dari hasil penelitian ini, terlihat kemampuan air tebu mampu mempertahankan motilitas spermatozoa sampai dengan dua hari.Kata Kunci: air tebu, sapi persilangan, spermatozoa asal kauda epididimisABSTRACT Sugarcane juice is known to contain sucrose which can increase energy for spermatozoa motility. The object of the study was to determine the ability of sugarcane juice and egg yolk in maintaining the quality of spermatozoa from cauda epididymis of crossbred cattle. This study used epididymal of crossing cattle from the slaughterhouse of Basirih - Banjarmasin. The collected spermatozoa were divided into four test tubes with the same volume in according to the number of treatments i.e.: 80% lactose + 20% egg yolk (control), 45% sugarcane + 45% aquabidest +10% egg yolk (ATKT10), 42.5% sugarcane + 42.5% aquabidest +15% egg yolk (ATKT15), and 40% sugarcane + 40% aquabidest +20% egg yolk (ATKT20). The parameter observed included percentage of motility, viability and intact plasma membrane (IPM). The results showed that (1) the percentage of motility (65%) and spermatozoa viability (79.60%) of control were higher (P 0.05) than in other treatments, however, ATKT20 had a higher percentage of both parameters than in ATKT10 and ATKT15; (2) the percentage of IPM control (76,4%) was not significantly different (P0,05) to ATKT20 (76%). Both treatments have higher of IPM than ATKT10 (71.80%) and ATKT15 (74.20%). The sugarcane juice was able to maintain spermatozoa motility for up to two days.Keywords: crossbred cattle, spermatozoa from cauda epididymis, sugarcane juice

Collaboration network of the authors

8 authors · 11 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Lambung Mangkurat University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Akbar BudiansaLambung Mangkurat University2
Jacky Setiofan CoesterLambung Mangkurat University1
Abrani SulaimanLambung Mangkurat University1
Mihammad RizalAffiliation unknown1
Anis WahdiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/peternakan-tropis/oai, first harvested 29 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Motilitas Dan Daya Hidup Spermatozoa Asal Epididimis Sapi Persilangan yang Diencerkan dengan Air Tebu</dc:title>
	<dc:creator>Riyadhi, Muhammad</dc:creator>
	<dc:creator>Rizal, Muhammad</dc:creator>
	<dc:creator>Thahir, Muhammad</dc:creator>
	<dc:description xml:lang="en-US">ABSTRAKAir tebu diketahui mengandung sukrosa yang dapat meningkatkan energi bagi motilitas spermatozoa.  Penelitian ini bertujuan untuk mengetahui kemampuan air tebu dan kuning telur dalam mempertahankan kualitas spermatozoa asal kauda epididymis sapi persilangan.  Penelitian ini menggunakan epididimis sapi persilangan dari rumah pemotongan hewan (RPH) Basirih Kotamadya Banjarmasin.  Spermatozoa yang dikoleksi ditempatkan ke dalam empat tabung reaksi masing-masing terdiri dari: 80% pengencer laktosa + 20% kuning telur ayam ras (kontrol), 45% air tebu + 45% akubidestilata + 10% kuning telur ayam ras (ATKT10), 42,5% air tebu + 42,5% akubidestilata + 15% kuning telur ayam ras (ATKT15), dan 40% air tebu + 40% akubidestilata + 20% kuning telur ayam ras (ATKT20). Parameter pengamatan adalah motilitas, daya hidup, dan membran plasma utuh.  Hasil penelitian menunjukkan motilitas pengencer kontrol (65%), berbeda nyata (P0,05) dengan ATKT10 (49%), ATKT15 (48%) dan ATKT20 (54%), namun demikian ATKT20 masih lebih tinggi (P0,05) dengan ATKT10 dan ATKT15.  Persentase daya hidup spermatozoa berbeda nyata (P0,05) antara kontrol (79,60%) dengan ATKT10 (72,40%), ATKT15 (73,20%), dan ATKT20 (74,60%), akan tetapi ATKT20 dan ATKT15 masih lebih baik (P0,05) dari ATKT10.  Untuk persentase MPU, kontrol sebesar 76,4%, sama dengan (P0,05) perlakuan ATKT 20 sebesar 76%, akan tetapi persentase MPU kontrol masih lebih baik (P0,05) dari ATKT 10 sebesar 71,80% dan ATKT15 sebesar 74,20%.  Dari hasil penelitian ini, terlihat kemampuan air tebu mampu mempertahankan motilitas spermatozoa sampai dengan dua hari.Kata Kunci : air tebu, sapi persilangan, spermatozoa asal kauda epididimisABSTRACT          Sugarcane juice is known to contain sucrose which can increase energy for spermatozoa motility.  The object of the study was to determine the ability of sugarcane juice and egg yolk in maintaining the quality of spermatozoa from cauda epididymis of crossbred cattle. This study used epididymal of crossing cattle from the slaughterhouse of Basirih - Banjarmasin.  The collected spermatozoa were divided into four test tubes with the same volume in according to the number of treatments i.e. : 80% lactose + 20% egg yolk  (control), 45% sugarcane + 45% aquabidest +10% egg yolk (ATKT10), 42.5% sugarcane +  42.5% aquabidest +15% egg yolk (ATKT15), and 40% sugarcane + 40% aquabidest +20% egg yolk (ATKT20). The parameter observed included percentage of motility, viability and intact plasma membrane (IPM).  The results showed that (1) the percentage of motility (65%) and spermatozoa viability (79.60%) of control were higher (P 0.05) than in other treatments, however, ATKT20 had a higher percentage of both parameters than in ATKT10 and ATKT15; (2) the percentage of IPM control (76,4%) was not significantly different (P0,05) to ATKT20 (76%). Both treatments have higher of IPM than ATKT10 (71.80%) and ATKT15 (74.20%).  The sugarcane juice was able to maintain spermatozoa motility for up to two days.Keywords: crossbred cattle, spermatozoa from cauda epididymis, sugarcane juice</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-01-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/8855</dc:identifier>
	<dc:identifier>10.33772/jitro.v7i1.8855</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu dan Teknologi Peternakan Tropis; Vol 7, No 1 (2020): JITRO, Januari; 70-76</dc:source>
	<dc:source>2406-9337</dc:source>
	<dc:source>10.33772/jitro.v7i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/8855/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 Jurnal Ilmu dan Teknologi Peternakan Tropis</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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