Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin

Muhammad, Dedi; Isnaini, Nurul; Kuswati, Kuswati; Yekti, Aulia Puspita Anugra; Luthfi, Muchamad; Sunarto, Lukman Affandhy; Susilawati, Trinil

doi:10.33772/jitro.v6i1.5450

Open access Jurnal SINTA 2

Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin

JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) · Vol. 6 No. 1 (2019) · pp. 85–91

DOI: 10.33772/jitro.v6i1.5450
ISSN: 2406-9337
Published: 2019-01-21
Publisher: Universitas Halu Oleo
Language: eng
License: creativecommons.org/licenses/by-sa/4.0

Abstract

ABSTRAKPengencer dasar air kelapa merupakan solusi dari sulit dan mahalnya harga pengadaan bahan baku pembuatan pengencer. Tujuan dari penelitian ini adalah untuk mengetahui kapasitasi spermatozoa sapi Peranakan Ongole dalam berbagai formulasi pengencer air kelapa selama simpan dingin pada suhu 4-5oC. Metodologi yang digunakan adalah eksperimantal laboratorium menggunakan rancangan acak kelompok (RAK). Terdapat tiga perlakuan yaitu, P1 = Pengencer air kelapa + 20% kuning telur, P2 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 1000 mg/l. P3 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 2000 mg/l, dengan 10 ulangan. Hasil menunjukkan bahwa tidak terdapat perbedaan yang signifikan pada persentase spermatozoa belum kapasitasi, terkapasitasi dan telah rekasi akrosom antara perlakuan formulasi pengencer air kelapa yang berbeda (P1, P2, P3) selama peyimpana suhu 2-5oC. Pengencer berbasis air kelapa mampu mempertahankan kualitas akrosom diatas 50% penyimpanan hari ke lima.Kata kunci: air kelapa, kapasitasi, reaksi akrosom, semen cairABSTRACTCoconut water extender is the solution to the difficulty and high cots of semen diluents. The objective of this study were to examine the capacitation of Ongole Crossbreed sperm in various formulations of coconut water extender during storage in 4-5 oC. The study method used laboratory experimental. The experimental design was Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are three treatments in this study (P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were not significant differences in percentage of sperm uncapacitation, sperm capacitaion and sperm acrosom reaction between various formulations of coconut water diluents during chilled storage at 4-5 oC. Coconut water diluents are able to maintain quality of sperm acrosom up to day 5th with values above 50%.Keywords: acrosom reaction, capacitation, coconut water, liquid semen

Collaboration network of the authors

31 authors · 92 co-authorship links · 6 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

University of Brawijaya
Politeknik Pertanian Negeri Samarinda
Universiti Sains Malaysia
Affiliation unknown

Different organisations
Same organisation
Affiliation unknown
Thicker line = more joint articles · bigger circle = more articles

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Co-author	Organisation	Joint articles
Asri Nurul Huda	University of Brawijaya	7
Dian Ratnawati	University of Brawijaya	4
Jois Harsah	Affiliation unknown	4
Muhammad Dikman	Affiliation unknown	4
Willy Saputra Saputra Tatulus	Politeknik Pertanian Negeri Samarinda	3
L Affandhy	University of Brawijaya	3
Agus Susilo	Affiliation unknown	2
Wike Andre Septian	Affiliation unknown	2
Dony Herviyanto	Affiliation unknown	2
Muhammad Ade Salim	University of Brawijaya	2
Muhammad Nur Ihsan	University of Brawijaya	2
Brilla Ismaya Safitri	Affiliation unknown	2
Sri Wahjuningsih	Affiliation unknown	2
Henny Leondro	Affiliation unknown	2
Enik Dwi Kusumawati	Affiliation unknown	2
Noni Ashri Maghfiroh	Affiliation unknown	1
Madiyan Sugesti	Affiliation unknown	1
Shintia Sukmawati Cahya Maulid	Affiliation unknown	1
Didiek Purwanto	Affiliation unknown	1
Mukhtar Mukhtar	Universiti Sains Malaysia	1
Ikhsanuddin Ikhsanuddin	Affiliation unknown	1
V.M. Ani Nurgiartiningsih	University of Brawijaya	1
Lukman Affandhy	Affiliation unknown	1
Frediansyah Firdaus	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/peternakan-tropis/oai, first harvested 29 Okt 2026, last seen 1 Nov 2026.

Original metadata (oai_dc)

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	<dc:title xml:lang="en-US">Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin</dc:title>
	<dc:creator>Muhammad, Dedi</dc:creator>
	<dc:creator>Isnaini, Nurul</dc:creator>
	<dc:creator>Kuswati, Kuswati</dc:creator>
	<dc:creator>Yekti, Aulia Puspita Anugra</dc:creator>
	<dc:creator>Luthfi, Muchamad</dc:creator>
	<dc:creator>Sunarto, Lukman Affandhy</dc:creator>
	<dc:creator>Susilawati, Trinil</dc:creator>
	<dc:description xml:lang="en-US">ABSTRAKPengencer dasar air kelapa merupakan solusi dari sulit dan mahalnya harga pengadaan bahan baku pembuatan pengencer. Tujuan dari penelitian ini adalah untuk mengetahui kapasitasi spermatozoa sapi Peranakan Ongole dalam berbagai formulasi pengencer air kelapa selama simpan dingin pada suhu 4-5oC. Metodologi yang digunakan adalah eksperimantal laboratorium menggunakan rancangan acak kelompok (RAK). Terdapat tiga perlakuan yaitu, P1 = Pengencer air kelapa + 20% kuning telur, P2 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 1000 mg/l. P3 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 2000 mg/l, dengan 10 ulangan. Hasil menunjukkan bahwa tidak terdapat perbedaan yang signifikan pada persentase spermatozoa belum kapasitasi, terkapasitasi dan telah rekasi akrosom antara perlakuan formulasi pengencer air kelapa yang berbeda (P1, P2, P3) selama peyimpana suhu 2-5oC. Pengencer berbasis air kelapa mampu mempertahankan kualitas akrosom diatas 50% penyimpanan hari ke lima.Kata kunci : air kelapa, kapasitasi, reaksi akrosom, semen cairABSTRACTCoconut water extender is the solution to the difficulty and high cots of semen diluents. The objective of this study were to examine the capacitation of Ongole Crossbreed sperm in various formulations of coconut water extender during storage in 4-5 oC. The study method used laboratory experimental. The experimental design was Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are three treatments in this study (P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were not significant differences in percentage of sperm uncapacitation, sperm capacitaion and sperm acrosom reaction between various formulations of coconut water diluents during chilled storage at 4-5 oC. Coconut water diluents are able to maintain quality of sperm acrosom up to day 5th with values above 50%.Keywords: acrosom reaction, capacitation, coconut water, liquid semen</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Halu Oleo</dc:publisher>
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	<dc:date>2019-01-21</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/5450</dc:identifier>
	<dc:identifier>10.33772/jitro.v6i1.5450</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu dan Teknologi Peternakan Tropis; Vol 6, No 1 (2019): JITRO, Januari; 85-91</dc:source>
	<dc:source>2406-9337</dc:source>
	<dc:source>10.33772/jitro.v6i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/5450/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Jurnal Ilmu dan Teknologi Peternakan Tropis</dc:rights>
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