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Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions

Indonesian Journal of Cancer Chemoprevention · Vol. 10 No. 1 (2019) · hlm. 7–15

DOI
10.14499/indonesianjcanchemoprev10iss1pp7-15
ISSN
2355-8989, 2088-0197
Terbit
2019-02-28
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords: EPO, erythropoietin, protein expression, CHO-K1, optimation

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  • National Research and Innovation Agency
  • Bandung Institute of Technology
  • Indonesian Institute of Sciences
  • Universitas Gadjah Mada
  • IPB University
  • Tanjungpura University
  • PT Bio Farma (Indonesia)
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Rekan penulisOrganisasiArtikel bersama
Pekik Wiji PrasetyaningrumNational Research and Innovation Agency14
Yana RubiyanaNational Research and Innovation Agency12
Ratna Dwi RamadaniNational Research and Innovation Agency9
Ratih Asmana NingrumNational Research and Innovation Agency8
Ahmad SuyokoNational Research and Innovation Agency6
Dian Fitria AgustiyantiNational Research and Innovation Agency4
Andri WardianaNational Research and Innovation Agency4
Khairul AnamNational Research and Innovation Agency4
Neny NurainiPT Bio Farma (Indonesia)4
Edy MeiyantoUniversitas Gadjah Mada3
Ria Fajarwati KastianNational Research and Innovation Agency3
Komang Alit ParamitasariNational Research and Innovation Agency3
Alayna Lillahida Indri HimawanNational Research and Innovation Agency3
Salsabila Putri AzzahraIndonesia Defense University3
Neng HerawatiNational Research and Innovation Agency3
Ernawati Arifin Giri-RachmanBandung Institute of Technology2
Hariyatun HariyatunIndonesian Institute of Sciences2
Fitri Aulia PermatasariBandung Institute of Technology2
Ferry IskandarBandung Institute of Technology2
Marissa AngelinaNational Research and Innovation Agency2
Azzania FibrianiBandung Institute of Technology2
Ratna Asmah SusidartiUniversitas Gadjah Mada2
Indri KusharyantiTanjungpura University2
Metta NoviantiNational Research and Innovation Agency2
Wisnu Ananta KusumaIPB University1
Annisa AnnisaIPB University1
Desriani DesrianiIndonesian Institute of Sciences1
Audrey Angelina Putri TaharuddinBandung Institute of Technology1
Nicholas YamahokiBandung Institute of Technology1
Rebecca StephanieBandung Institute of Technology1
Arum Sinda SantikaBandung Institute of Technology1
Nahdiya Laila MufidaBandung Institute of Technology1
Retno Damajanti SoejoedonoIPB University1
Nur Faiizah Aqiilah FirmanAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions</dc:title>
	<dc:creator>Santoso, Adi</dc:creator>
	<dc:creator>Larasati, Larasati</dc:creator>
	<dc:creator>Kusumawati, Arizah</dc:creator>
	<dc:creator>Wisnuwardhani, Popi Hadi</dc:creator>
	<dc:creator>Ningrum, Ratih Asma</dc:creator>
	<dc:creator>Septisetyani, Endah Puji</dc:creator>
	<dc:description xml:lang="en-US">Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US">Indonesian Institute of Sciences</dc:contributor>
	<dc:date>2019-02-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/261</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev10iss1pp7-15</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 10, No 1 (2019); 7-15</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/261/176</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Adi Santoso</dc:rights>
</oai_dc:dc>