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Effect of Thawing Technique on the Quality and Status of Acrosome Reactions

JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) · Vol. 9 No. 2 (2022) · pp. 437–445

DOI
10.33772/jitro.v9i2.23126
ISSN
2406-9337
Published
2022-05-31
Publisher
Universitas Halu Oleo
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

The success of Artificial Insemination is affected by the thawing technique used by the inseminator. The difference in thawing technique can affect the quality of the spermatozoa produced. This study aims to determine the effect of temperature and duration of thawing on the frozen semen quality of Bali and Madura cattle. The material used was 80 frozen semen straws for Bali cattle and Madura cattle. The method used is an experiment with four treatments and 10 replications, that is thawing in water at 37℃ for 30 seconds duration, 28℃ for 30 seconds, 28℃ for 45 seconds and 28℃ for 60 seconds. The variables observed in this study were capacitation, intact acrosome, intact plasma membrane and chromatin damage. The data were analyzed using a Randomized Block Design with replication as a block. The results of the analysis of variance showed that thawing technique had significant effect (p0.05) on not yet capacitated, intact acrosome, intact plasma membrane. Thawing technique had no significant effect (p0.05) on the acrosome reacted and chromatin damage.Keywords: thawing technique, capacitation, intact acrosome, intact plasma membrane, chromatin damage

Collaboration network of the authors

23 authors · 75 co-authorship links · 5 across different organisations

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  • University of Brawijaya
  • Politeknik Pertanian Negeri Samarinda
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Kuswati KuswatiAffiliation unknown7
Nurul IsnainiUniversity of Brawijaya4
Muchamad LuthfiAffiliation unknown4
Asri Nurul HudaUniversity of Brawijaya4
Dedi MuhammadUniversity of Brawijaya2
Lukman Affandhy SunartoAffiliation unknown2
Willy Saputra Saputra TatulusPoliteknik Pertanian Negeri Samarinda2
Dian RatnawatiUniversity of Brawijaya2
L AffandhyUniversity of Brawijaya2
Jois HarsahAffiliation unknown2
Muhammad DikmanAffiliation unknown2
Wike Andre SeptianAffiliation unknown1
Dony HerviyantoAffiliation unknown1
Muhammad Ade SalimUniversity of Brawijaya1
Muhammad Nur IhsanUniversity of Brawijaya1
Reza FahleviAffiliation unknown1
Mohammad Nur IhsanAffiliation unknown1
Henny LeondroAffiliation unknown1
Enik Dwi KusumawatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/peternakan-tropis/oai, first harvested 29 Okt 2026, last seen 29 Okt 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of Thawing Technique on the Quality and Status of Acrosome Reactions</dc:title>
	<dc:creator>Safitri, Brilla Ismaya</dc:creator>
	<dc:creator>Wahjuningsih, Sri</dc:creator>
	<dc:creator>Yekti, Aulia Puspita Anugra</dc:creator>
	<dc:creator>Susilawati, Trinil</dc:creator>
	<dc:description xml:lang="en-US">The success of Artificial Insemination is affected by the thawing technique used by the inseminator. The difference in thawing technique can affect the quality of the spermatozoa produced. This study aims to determine the effect of temperature and duration of thawing on the frozen semen quality of Bali and Madura cattle. The material used was 80 frozen semen straws for Bali cattle and Madura cattle. The method used is an experiment with four treatments and 10 replications, that is thawing in water at 37℃ for 30 seconds duration, 28℃ for 30 seconds, 28℃ for 45 seconds and 28℃ for 60 seconds. The variables observed in this study were capacitation, intact acrosome, intact plasma membrane and chromatin damage.  The data were analyzed using a Randomized Block Design with replication as a block. The results of the analysis of variance showed that thawing technique had significant effect (p0.05) on not yet capacitated, intact acrosome, intact plasma membrane.  Thawing technique had no significant effect (p0.05) on the acrosome reacted and chromatin damage.Keywords:  thawing technique, capacitation, intact acrosome, intact plasma membrane, chromatin damage</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2022-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/23126</dc:identifier>
	<dc:identifier>10.33772/jitro.v9i2.23126</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu dan Teknologi Peternakan Tropis; Vol 9, No 2 (2022): JITRO, May 2022; 437-445</dc:source>
	<dc:source>2406-9337</dc:source>
	<dc:source>10.33772/jitro.v9i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/23126/pdf</dc:relation>
	<dc:relation>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/downloadSuppFile/23126/4465</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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