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Hypoxia-Induced Mesenchymal Stem Cell Exosomes Promote PDGF and IL-10 Expression During Burn Wound Healing

Journal of Biomedicine and Translational Research · Vol. 12 No. 2 (2026) · hlm. 86–94

DOI
10.14710/jbtr.v12i2.28892
ISSN
2503-2178
Terbit
2026-08-31
Penerbit
Faculty of Medicine, Universitas Diponegoro
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Background: Burn injuries remain a global health concern, with third-degree burns posing high morbidity and mortality risks. Effective wound healing requires both regeneration and inflammation control. Hypoxia-induced mesenchymal stem cell-derived exosomes (EH-MSCs) have emerged as a promising cell-free therapy due to their regenerative and immunomodulatory properties.Objective: This study aimed to evaluate the effect of EH-MSCs on the expression of Platelet-Derived Growth Factor (PDGF) and Interleukin-10 (IL-10) in a Wistar rat model of third-degree burns.Methods: An experimental study with a Post-Test Only Control Group design was conducted at the Stem Cell and Cancer Research Laboratory, Sultan Agung Islamic University, Semarang, in April 2025. Thirty male Wistar rats (6–8 weeks, 200–250 g) were randomized into five groups: healthy control, burn + NaCl, burn + silver sulfadiazine, burn + EH-MSC 100 µg/mL, and burn + EH-MSC 200 µg/mL. Third-degree burns were induced using a 2 × 2 cm² pre-heated metal plate under ether anesthesia. EH-MSCs were isolated from rat umbilical cords using tangential flow filtration and validated with CD63 and CD9 markers. On day 5, PDGF and IL-10 expressions were measured via RT-PCR after orbital sinus blood sampling. Data were analyzed using Shapiro-Wilk and Levene’s tests, followed by One-Way ANOVA or non-parametric equivalents, with p<0.05 considered significant.Results: PDGF and IL-10 expression increased in EH-MSC-treated groups, with the highest levels in the 200 µg/mL dose. IL-10 showed a significant difference among groups (p = 0.030), and post hoc tests confirmed higher expression in EH-MSC groups. PDGF showed a significant pairwise difference, though overall ANOVA was not significant (p = 0.094).Conclusion: EH-MSCs significantly enhanced IL-10 expression and increased PDGF levels, supporting their potential to promote regeneration and modulate inflammation in burn wound healing.

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26 penulis · 57 tautan kepenulisan bersama · 8 lintas organisasi

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  • Sultan Agung Islamic University
  • Diponegoro University
  • RSUD Prof. Dr. Saiful Anwar Malang
  • Universitas Sumatera Utara
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Sri Priyantini MulyaniSultan Agung Islamic University1
Yoshi PuspitasariSultan Agung Islamic University1
Hamzah HamzahAfiliasi tidak diketahui1
Thomas Erwin Christian Junus HuwaeRSUD Prof. Dr. Saiful Anwar Malang1
Nur Anna Chalimah Sa'dyahAfiliasi tidak diketahui1
Agung Brastama PutraSultan Agung Islamic University1
Setyo TrisnadiSultan Agung Islamic University1
Hadi SunartoSultan Agung Islamic University1
Arya TjiptaUniversitas Sumatera Utara1
Harvina Dwi AprilianaSultan Agung Islamic University1
Eko SetiawanSultan Agung Islamic University1
Sri PriyantiniSultan Agung Islamic University1
Pinandhito Nararya Wirrya LatukolanSultan Agung Islamic University1
Taufiqurrahman NasihunSultan Agung Islamic University1
Eni WidayatiSultan Agung Islamic University1
Hardhono SusantoDiponegoro University1
Edi DharmanaDiponegoro University1
Sarjadi SarjadiDiponegoro University1
Hadi SarosaSultan Agung Islamic University1
Danis PertiwiSultan Agung Islamic University1
Atina HussaanaSultan Agung Islamic University1
Ulfah Dian IndrayaniSultan Agung Islamic University1
Bagas WidiyantoSultan Agung Islamic University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ejournal2.undip.ac.id/index.php/jbtr/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Hypoxia-Induced Mesenchymal Stem Cell Exosomes Promote PDGF and IL-10 Expression During Burn Wound Healing</dc:title>
	<dc:creator>Yuniar, Fanni</dc:creator>
	<dc:creator>Setiawan, Eko</dc:creator>
	<dc:creator>Chodidjah, Chodidjah</dc:creator>
	<dc:subject xml:lang="en-US">burn wound; mesenchymal stem cells; exosomes; PDGF; IL-10</dc:subject>
	<dc:description xml:lang="en-US">Background: Burn injuries remain a global health concern, with third-degree burns posing high morbidity and mortality risks. Effective wound healing requires both regeneration and inflammation control. Hypoxia-induced mesenchymal stem cell-derived exosomes (EH-MSCs) have emerged as a promising cell-free therapy due to their regenerative and immunomodulatory properties.Objective: This study aimed to evaluate the effect of EH-MSCs on the expression of Platelet-Derived Growth Factor (PDGF) and Interleukin-10 (IL-10) in a Wistar rat model of third-degree burns.Methods: An experimental study with a Post-Test Only Control Group design was conducted at the Stem Cell and Cancer Research Laboratory, Sultan Agung Islamic University, Semarang, in April 2025. Thirty male Wistar rats (6–8 weeks, 200–250 g) were randomized into five groups: healthy control, burn + NaCl, burn + silver sulfadiazine, burn + EH-MSC 100 µg/mL, and burn + EH-MSC 200 µg/mL. Third-degree burns were induced using a 2 × 2 cm² pre-heated metal plate under ether anesthesia. EH-MSCs were isolated from rat umbilical cords using tangential flow filtration and validated with CD63 and CD9 markers. On day 5, PDGF and IL-10 expressions were measured via RT-PCR after orbital sinus blood sampling. Data were analyzed using Shapiro-Wilk and Levene’s tests, followed by One-Way ANOVA or non-parametric equivalents, with p&lt;0.05 considered significant.Results: PDGF and IL-10 expression increased in EH-MSC-treated groups, with the highest levels in the 200 µg/mL dose. IL-10 showed a significant difference among groups (p = 0.030), and post hoc tests confirmed higher expression in EH-MSC groups. PDGF showed a significant pairwise difference, though overall ANOVA was not significant (p = 0.094).Conclusion: EH-MSCs significantly enhanced IL-10 expression and increased PDGF levels, supporting their potential to promote regeneration and modulate inflammation in burn wound healing.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Medicine, Universitas Diponegoro</dc:publisher>
	<dc:date>2026-08-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal2.undip.ac.id/index.php/jbtr/article/view/28892</dc:identifier>
	<dc:identifier>10.14710/jbtr.v12i2.28892</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Biomedicine and Translational Research; Vol 12, No 2 (2026): August 2026; 86-94</dc:source>
	<dc:source>2503-2178</dc:source>
	<dc:source>10.14710/jbtr.v12i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal2.undip.ac.id/index.php/jbtr/article/view/28892/14863</dc:relation>
	<dc:relation>https://ejournal2.undip.ac.id/index.php/jbtr/article/downloadSuppFile/28892/7115</dc:relation>
	<dc:relation>https://ejournal2.undip.ac.id/index.php/jbtr/article/downloadSuppFile/28892/7117</dc:relation>
	<dc:relation>https://ejournal2.undip.ac.id/index.php/jbtr/article/downloadSuppFile/28892/8573</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Journal of Biomedicine and Translational Research</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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