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Screening and profiling of mercury-resistant Azotobacter isolated from gold mine tailing in Pongkor, West Java

Journal of Degraded and Mining Lands Management · Vol. 11 No. 2 (2024) · hlm. 5287–5300

DOI
10.15243/jdmlm.2024.112.5287
ISSN
2502-2458, 2339-076X
Terbit
2024-01-01
Penerbit
Brawijaya University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Mercury (Hg) is a hazardous pollutant produced during the amalgamation of gold extraction. The environmental problems related to improper Hg waste management have become progressively concerning. Hg contamination in environments can be removed by using bioremediation technology. Utilizing Hg-resistant (HgR) microorganisms in Hg bioremediation is a crucial strategy. Azotobacter is one of the potential microbes for Hg bioremediation bioagent due to exopolysaccharides synthesis that binds the heavy metal. The study's main objective was to select and profile a novel Hg-resistant Azotobacter isolated from heavily Hg-contaminated soil and tailing of artisanal and small-scale gold in Pongkor area, West Java, Indonesia. The completely randomized design was used for profiling Azotobacter-HgR and included Hg values of 0, 1, 10, 100, 200, and 400 mg/L. Further, Azotobacter isolate bioassay steps included soil contaminated with Hg, soil contaminated with Hg + Azotobacter sp. S6.a, soil contaminated with Hg + consortium. The profiling results revealed that four Hg-resistant isolates were Azotobacter sp. S5, Azotobacter sp. S6, Azotobacter sp. S6.a, and Azotobacter sp. S9. More importantly, Azotobacter sp. S5 followed by Azotobacter sp. S6.a was found to be the most resistant to Hg exposure at a concentration of 400 mg/L. The Azotobacter sp. S9. produced the lowest EPS, but had the highest activity of nitrogenase and organic acid production. Meanwhile, Azotobacter sp. S6.a. produced the highest EPS. Isolate S5 showed the highest potential as a resistant PGPR-Hg isolate for enhancing the growth of sorghum in Hg-contaminated soil. Sorghum plants accumulate Hg from the soil in the roots but not in the shoots. Present findings suggest that these two isolates have the potential to be used as biological agents to rehabilitate Hg-contaminated soil in Pongkor area.

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  • Padjadjaran University
  • Universitas Jember
  • National Research and Innovation Agency
  • Siliwangi University
  • Universitas Pattimura
  • Universitas Islam Negeri Ar-Raniry
  • Kementerian Energi dan Sumber Daya Mineral
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Rekan penulisOrganisasiArtikel bersama
Mieke Rochimi SetiawatiPadjadjaran University12
Nadia Nuraniya KamaluddinPadjadjaran University7
Betty Natalie FitriatinPadjadjaran University5
Agung KaruniawanPadjadjaran University5
Diyan HerdiyantoroPadjadjaran University4
Triyani DewiNational Research and Innovation Agency4
Emma Trinurani SofyanPadjadjaran University3
Rija SudirjaPadjadjaran University3
Syafrizal SyafrizalUniversitas Islam Negeri Ar-Raniry3
Zulkifliani ZulkiflianiAfiliasi tidak diketahui3
Nia KurniatiPadjadjaran University2
Mahfud ArifinPadjadjaran University2
Agustinus Marthin KalayUniversitas Pattimura2
Rhazista NoviardiNational Research and Innovation Agency2
Tualar SimarmataPadjadjaran University2
Andi Hana Mufidah ElmirasariPadjadjaran University2
Jajang Sauman HamdaniPadjadjaran University2
Sondi KuswaryanPadjadjaran University1
Rustam RustamKementerian Energi dan Sumber Daya Mineral1
Dadang Epi SukarsaPadjadjaran University1
Tarra Martiana DewiPadjadjaran University1
Anne NurbaityPadjadjaran University1
Lia Nur LindaPadjadjaran University1
Andina ChotimahAfiliasi tidak diketahui1
Asana MatsuuraShinshu University1
Yeni Wispa DewiPadjadjaran University1
Yaya SunaryaSiliwangi University1
Rudi PriyadiSiliwangi University1
Rara Rahmatika RisantiPadjadjaran University1
Fasa AdityaAfiliasi tidak diketahui1
Gita Bina NugrahaAfiliasi tidak diketahui1
ELENA F. L. LILIPALYUniversitas Jember1
Iis Nur AsyiahUniversitas Jember1
Imam MudakirUniversitas Jember1
Rita HarniUniversitas Jember1
Muhammad FauziPadjadjaran University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jdmlm.ub.ac.id/index.php/jdmlm/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Screening and profiling of mercury-resistant Azotobacter isolated from gold mine tailing in Pongkor, West Java</dc:title>
	<dc:creator>Suryatmana, Pujawati</dc:creator>
	<dc:creator>Handayani, Sri</dc:creator>
	<dc:creator>Bang, Sunbaek</dc:creator>
	<dc:creator>Hindersah, Reginawanti</dc:creator>
	<dc:subject xml:lang="en-US">ASGM</dc:subject>
	<dc:subject xml:lang="en-US">Azotobacter</dc:subject>
	<dc:subject xml:lang="en-US">exopolysaccharide</dc:subject>
	<dc:subject xml:lang="en-US">Hg resistant microbes</dc:subject>
	<dc:description xml:lang="en-US">Mercury (Hg) is a hazardous pollutant produced during the amalgamation of gold extraction. The environmental problems related to improper Hg waste management have become progressively concerning. Hg contamination in environments can be removed by using bioremediation technology. Utilizing Hg-resistant (HgR) microorganisms in Hg bioremediation is a crucial strategy. Azotobacter is one of the potential microbes for Hg bioremediation bioagent due to exopolysaccharides synthesis that binds the heavy metal. The study's main objective was to select and profile a novel Hg-resistant Azotobacter isolated from heavily Hg-contaminated soil and tailing of artisanal and small-scale gold in Pongkor area, West Java, Indonesia. The completely randomized design was used for profiling Azotobacter-HgR and included Hg values of 0, 1, 10, 100, 200, and 400 mg/L. Further, Azotobacter isolate bioassay steps included soil contaminated with Hg, soil contaminated with Hg + Azotobacter sp. S6.a, soil contaminated with Hg + consortium. The profiling results revealed that four Hg-resistant isolates were Azotobacter sp. S5, Azotobacter sp. S6, Azotobacter sp. S6.a, and Azotobacter sp. S9. More importantly, Azotobacter sp. S5 followed by Azotobacter sp. S6.a was found to be the most resistant to Hg exposure at a concentration of 400 mg/L. The Azotobacter sp. S9. produced the lowest EPS, but had the highest activity of nitrogenase and organic acid production. Meanwhile, Azotobacter sp. S6.a. produced the highest EPS. Isolate S5 showed the highest potential as a resistant PGPR-Hg isolate for enhancing the growth of sorghum in Hg-contaminated soil. Sorghum plants accumulate Hg from the soil in the roots but not in the shoots. Present findings suggest that these two isolates have the potential to be used as biological agents to rehabilitate Hg-contaminated soil in Pongkor area.</dc:description>
	<dc:publisher xml:lang="en-US">Brawijaya University</dc:publisher>
	<dc:date>2024-01-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/7548</dc:identifier>
	<dc:identifier>10.15243/jdmlm.2024.112.5287</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Degraded and Mining Lands Management; Vol. 11 No. 2 (2024); 5287-5300</dc:source>
	<dc:source>2502-2458</dc:source>
	<dc:source>2339-076X</dc:source>
	<dc:source>10.15243/jdmlm.2023.112</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/7548/908</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Journal of Degraded and Mining Lands Management</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>