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Degradation of diesel fuel by Pseudomonas aeruginosa B031 with expression of the alkB gene in a column bioreactor

Journal of Degraded and Mining Lands Management · Vol. 12 No. 1 (2024) · hlm. 6875–6885

DOI
10.15243/jdmlm.2024.121.6875
ISSN
2502-2458, 2339-076X
Terbit
2024-10-01
Penerbit
Brawijaya University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Diesel fuel pollutants contain toxic hydrocarbons dominated by aliphatic and aromatic hydrocarbons. Hydrocarbon pollutants can be degraded in an environmentally friendly manner through bioremediation using hydrocarbonoclastic bacteria, i.e., Pseudomonas aeruginosa B031, which harbors the alkB gene that encodes an alkane hydroxylase that degrades alkane chains in hydrocarbons. This study compared the ability and efficiency of P. aeruginosa B031 to degrade diesel pollutants in a batch system and a continuous system using a column bioreactor, as well as the expression of alkB. P. aeruginosa B031 could more efficiently degrade diesel fuel in a continuous system in a column bioreactor than in the batch system. The concentrations of phenol, total organic carbon, chemical oxygen demand, and biological oxygen demand in the column bioreactor underwent a greater decrease than those in the batch system, namely 1.5-fold, 1.7-fold, 1.4-fold, and 1.3-fold, respectively. The decrease in these concentrations was followed by changes in functional groups, as shown via Fourier transform infrared spectroscopy. The number of bacteria and the concentration of exopolysaccharide increased in the column bioreactor by 4-fold and 2.3-fold more than the increase in the batch system. The ability of P. aeruginosa B031 to degrade diesel fuel in the column bioreactor was also demonstrated by the higher expression of alkB than that in the control.

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  • Universitas Gadjah Mada
  • Yogyakarta State University
  • Ministry of Public Works, Republic of Indonesia
  • Universitas Muhammadiyah Semarang
  • Indonesia Open University
  • National Research and Innovation Agency
  • Kementerian Energi dan Sumber Daya Mineral
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Rekan penulisOrganisasiArtikel bersama
Doni Prakasa Eka PutraUniversitas Gadjah Mada8
Hendy SetiawanUniversitas Gadjah Mada3
Laurentius Hartanto NugrohoUniversitas Gadjah Mada2
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Heru HendrayanaUniversitas Gadjah Mada2
Raja SusatioUniversitas Gadjah Mada2
Teuku Faisal FathaniUniversitas Gadjah Mada2
Pulung Arya PranantyaMinistry of Public Works, Republic of Indonesia2
Ketut Arte WidaneUniversitas Gadjah Mada1
Annisaa WidyasariUniversitas Gadjah Mada1
Sukirno SukirnoUniversitas Gadjah Mada1
Syarafina Ratna PutriUniversitas Gadjah Mada1
Sri DarmawatiUniversitas Muhammadiyah Semarang1
Muhammad Evy PrastiyantoUniversitas Gadjah Mada1
Wayan Tunas ArtamaUniversitas Gadjah Mada1
Fitri NadifahUniversitas Gadjah Mada1
Whika Febria DewatisariIndonesia Open University1
Yekti Asih PurwestriUniversitas Gadjah Mada1
Yuli WidiyastutiNational Research and Innovation Agency1
Ratna Asmah SusidartiUniversitas Gadjah Mada1
Rozana ZuhriUniversitas Gadjah Mada1
Restu Dwi Cahyo AdiUniversitas Gadjah Mada1
R ResiliKementerian Energi dan Sumber Daya Mineral1
Ahmad Tawfiequrrahman YuliansyahUniversitas Gadjah Mada1
H HusnizarUniversitas Gadjah Mada1
Phyu Phyu ThinUniversitas Gadjah Mada1
Satoru KawasakiHokkaido University1
Kurnianto Dwi SetyawanUniversitas Gadjah Mada1
R RisantiUniversitas Gadjah Mada1
Galeh Nur Indriatno Putra PratamaYogyakarta State University1
Slamet WidodoYogyakarta State University1
Maris Setyo NugrohoYogyakarta State University1
Wisnu Rachmad PrihadiYogyakarta State University1
La Ode Muhamad SyawaluddinUniversitas Gadjah Mada1
Surya Damar Sasongko PutroAfiliasi tidak diketahui1
I Gde Budi IndrawanUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jdmlm.ub.ac.id/index.php/jdmlm/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Degradation of diesel fuel by Pseudomonas aeruginosa B031 with expression of the alkB gene in a column bioreactor </dc:title>
	<dc:creator>Daris, Pieter Jhon Joshua</dc:creator>
	<dc:creator>Wilopo, Wahyu</dc:creator>
	<dc:creator>Warmada, Wayan</dc:creator>
	<dc:creator>Retnaningrum, Endah</dc:creator>
	<dc:subject xml:lang="en-US">alkB gene</dc:subject>
	<dc:subject xml:lang="en-US">degradation efficiency</dc:subject>
	<dc:subject xml:lang="en-US">exopolysaccharide</dc:subject>
	<dc:subject xml:lang="en-US">FTIR</dc:subject>
	<dc:subject xml:lang="en-US">gene expression</dc:subject>
	<dc:description xml:lang="en-US">Diesel fuel pollutants contain toxic hydrocarbons dominated by aliphatic and aromatic hydrocarbons. Hydrocarbon pollutants can be degraded in an environmentally friendly manner through bioremediation using hydrocarbonoclastic bacteria, i.e., Pseudomonas aeruginosa B031, which harbors the alkB gene that encodes an alkane hydroxylase that degrades alkane chains in hydrocarbons. This study compared the ability and efficiency of P. aeruginosa B031 to degrade diesel pollutants in a batch system and a continuous system using a column bioreactor, as well as the expression of alkB. P. aeruginosa B031 could more efficiently degrade diesel fuel in a continuous system in a column bioreactor than in the batch system. The concentrations of phenol, total organic carbon, chemical oxygen demand, and biological oxygen demand in the column bioreactor underwent a greater decrease than those in the batch system, namely 1.5-fold, 1.7-fold, 1.4-fold, and 1.3-fold, respectively. The decrease in these concentrations was followed by changes in functional groups, as shown via Fourier transform infrared spectroscopy. The number of bacteria and the concentration of exopolysaccharide increased in the column bioreactor by 4-fold and 2.3-fold more than the increase in the batch system. The ability of P. aeruginosa B031 to degrade diesel fuel in the column bioreactor was also demonstrated by the higher expression of alkB than that in the control.</dc:description>
	<dc:publisher xml:lang="en-US">Brawijaya University</dc:publisher>
	<dc:date>2024-10-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/16798</dc:identifier>
	<dc:identifier>10.15243/jdmlm.2024.121.6875</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Degraded and Mining Lands Management; Vol. 12 No. 1 (2024); 6875-6885</dc:source>
	<dc:source>2502-2458</dc:source>
	<dc:source>2339-076X</dc:source>
	<dc:source>10.15243/jdmlm.2024.121</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/16798/1186</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Journal of Degraded and Mining Lands Management</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>