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Marmin of Aegle Marmelos Correa Antagonizes AChM3 Receptors: In Silico and In Vitro Studies on Isolated-Guinea Pig Illeum Smooth Muscle

Journal of Mathematical and Fundamental Sciences · Vol. 46 No. 3 (2014) · hlm. 278–289

DOI
10.5614/j.math.fund.sci.2014.46.3.6
ISSN
2338-5510, 2337-5760
Terbit
2014-12-01
Penerbit
Directorate for Research and Innovation (DRI) ITB
Bahasa
eng

Abstrak

Marmin is a coumarin derivative compounds isolated from the bark and roots of Aegle Marmelos Correa. Previously, ethanolic extract of leaves of Aegle Marmelos Correa on isolated ileum showed antagonism effect on the contraction induced by histamine. Besides H1 receptor, there is also muscarinic acetylcholine (AChM)receptors involved in the mechanism of ileum contraction. Marmin is predicted to antagonize on AChM3 receptors.This study aimed to determine the effect of marmin on smooth muscle contraction of isolated guinea pig ileum induced by metacholine, an agonist of AChM3 receptor. The method in the study were in vitro (isolated organ) and in silico (docking PLANTS). In the in vitro study, marmin exhibited a competitive antagonists effect at the concentration of 100 μM (pA2 value of 1,728) on the AChM3 receptors. Reversibility assay on AChM3 receptors showed that by washing the ileum with Tyrode buffer every 6 minutes for 30 minutes, the bond of marmin to the receptor was still not completely detached. In the in silico study, marmin was observed to be bound to AchM3 receptors (docking score: -102.086). Marmin bond was weaker than that of native ligand of AChM3 receptor (tiotropium, docking score: -115.107), however, stronger than that of AChM3 receptor agonist and antagonist. Based on the results, we conclude that marmin have a competitive antagonist activity on AChM3 receptor.

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  • Universitas Gadjah Mada
  • Muhammadiyah University of Yogyakarta
  • National Research and Innovation Agency
  • Universitas Ahmad Dahlan
  • Atma Jaya Catholic University of Indonesia
  • Universitas Jember
  • Rumah Sakit Umum Pusat Dr. Sardjito
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Rekan penulisOrganisasiArtikel bersama
S. SardjimanAfiliasi tidak diketahui1
Kazutaka MaeyamaAfiliasi tidak diketahui1
Subagus WahyuonoUniversitas Gadjah Mada1
Yuli WidiyastutiNational Research and Innovation Agency1
Rarastoeti PratiwiUniversitas Gadjah Mada1
Raymond R. TjandrawinataAtma Jaya Catholic University of Indonesia1
Suwijiyo PramonoUniversitas Gadjah Mada1
Novia Tri AstutiUniversitas Gadjah Mada1
Putri Rachma NovitasariUniversitas Ahmad Dahlan1
Endah PuspitasariUniversitas Jember1
Endah Dwi SetyowatiRumah Sakit Umum Pusat Dr. Sardjito1
Binar Asrining DhianiMuhammadiyah University Purwokerto1
Edy MeiyantoUniversitas Gadjah Mada1
Anindyajati AnindyajatiUniversitas Gadjah Mada1
Ika NurzijahUniversitas Gadjah Mada1
Dita Brenna SeptheaUniversitas Gadjah Mada1
Andita Pra DarmaUniversitas Gadjah Mada1
Prajona MarbunAfiliasi tidak diketahui1
Endang LukitaningsihUniversitas Gadjah Mada1
Annik MegawatiUniversitas Gadjah Mada1
Arief NurrochmadUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journals.itb.ac.id/index.php/jmfs/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Marmin of Aegle Marmelos Correa Antagonizes AChM3 Receptors: In Silico and In Vitro Studies on Isolated-Guinea Pig Illeum Smooth Muscle</dc:title>
	<dc:creator>Nugroho, Agung Endro</dc:creator>
	<dc:creator>Noviarsito, Puguh</dc:creator>
	<dc:creator>Riyanto, Sugeng</dc:creator>
	<dc:description xml:lang="en-US">Marmin is a coumarin derivative compounds isolated from the bark and roots of Aegle Marmelos Correa. Previously, ethanolic extract of leaves of Aegle Marmelos Correa on isolated ileum showed antagonism effect on the contraction induced by histamine. Besides H1 receptor, there is also muscarinic acetylcholine (AChM)receptors involved in the mechanism of ileum contraction. Marmin is predicted to antagonize on AChM3 receptors.This study aimed to determine the effect of marmin on smooth muscle contraction of isolated guinea pig ileum induced by metacholine, an agonist of AChM3 receptor. The method in the study were in vitro (isolated organ) and in silico (docking PLANTS). In the in vitro study, marmin exhibited a competitive antagonists effect at the concentration of 100 μM (pA2 value of 1,728) on the AChM3 receptors. Reversibility assay on AChM3 receptors showed that by washing the ileum with Tyrode buffer every 6 minutes for 30 minutes, the bond of marmin to the receptor was still not completely detached. In the in silico study, marmin was observed to be bound to AchM3 receptors (docking score: -102.086). Marmin bond was weaker than that of native ligand of AChM3 receptor (tiotropium, docking score: -115.107), however, stronger than that of AChM3 receptor agonist and antagonist. Based on the results, we conclude that marmin have a competitive antagonist activity on AChM3 receptor.</dc:description>
	<dc:publisher xml:lang="en-US">Directorate for Research and Innovation (DRI) ITB</dc:publisher>
	<dc:date>2014-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journals.itb.ac.id/index.php/jmfs/article/view/783</dc:identifier>
	<dc:identifier>10.5614/j.math.fund.sci.2014.46.3.6</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Mathematical and Fundamental Sciences; Vol. 46 No. 3 (2014); 278-289</dc:source>
	<dc:source>2338-5510</dc:source>
	<dc:source>2337-5760</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journals.itb.ac.id/index.php/jmfs/article/view/783/771</dc:relation>
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