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Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease

Journal of Mathematical and Fundamental Sciences · Vol. 51 No. 3 (2019) · hlm. 309–319

DOI
10.5614/j.math.fund.sci.2019.51.3.8
ISSN
2338-5510, 2337-5760
Terbit
2019-12-20
Penerbit
Directorate for Research and Innovation (DRI) ITB
Bahasa
eng

Abstrak

PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.

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  • Universitas Gadjah Mada
  • Jenderal Soedirman University
  • Universitas Katolik Widya Mandira
  • University of Mataram
  • Universitas Jember
  • Universitas Airlangga
  • National University of Malaysia
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Rekan penulisOrganisasiArtikel bersama
Anjar Purba AsmaraUniversitas Islam Negeri Ar-Raniry2
Mudasir MudasirUniversitas Gadjah Mada2
Basuki RahmatIndonesia Defense University2
Sri Juari SantosaUniversitas Gadjah Mada2
Hildegardis MissaUniversitas Katolik Widya Mandira2
Anselmus Boy BaunseleUniversitas Katolik Widya Mandira2
Johnson N. NaatUniversity of Nusa Cendana2
Siti Raudhatul KamaliChaoyang University of Technology2
Ulul Khairi ZuryatiUniversity of Mataram2
Abdullah AbdullohAfiliasi tidak diketahui1
Didik Prasetyoko Didik PrasetyokoAfiliasi tidak diketahui1
Siti MaryamUniversitas Airlangga1
Triyono TriyonoUniversitas Gadjah Mada1
Wega TrisunaryantiUniversitas Gadjah Mada1
Nanik Siti AminahUniversitas Airlangga1
Bambang RusdiarsoUniversitas Gadjah Mada1
Jayus JayusAfiliasi tidak diketahui1
Fitria DamayantiState University of Semarang1
Aminah AbdullahNational University of Malaysia1
Lee Yook HengNational University of Malaysia1
Siti AstariUniversitas Gadjah Mada1
Suherman SuhermanUniversitas Gadjah Mada1
Busroni BusroniUniversitas Jember1
Chairil AnwarUniversitas Gadjah Mada1
Jumina JuminaUniversitas Gadjah Mada1
Khansa SalsabilaSebelas Maret University1
Uyi SulaemanJenderal Soedirman University1
Dadan HermawanJenderal Soedirman University1
Ponco IswantoJenderal Soedirman University1
Amin FatoniJenderal Soedirman University1
Cacu CacuJenderal Soedirman University1
Suwandri SuwandriJenderal Soedirman University1
Hassan Youssef Aboul-EneinAfiliasi tidak diketahui1
Erly Grizca BoelanUniversitas Katolik Widya Mandira1
Aloisius Masan KoponUniversitas Katolik Widya Mandira1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journals.itb.ac.id/index.php/jmfs/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease</dc:title>
	<dc:creator>Hermanto, Dhony</dc:creator>
	<dc:creator>Mudasir, Mudasir</dc:creator>
	<dc:creator>Siswanta, Dwi</dc:creator>
	<dc:creator>Kuswandi, Bambang</dc:creator>
	<dc:creator>Ismillayli, Nurul</dc:creator>
	<dc:subject xml:lang="en-US">alginate-chitosan</dc:subject>
	<dc:subject xml:lang="en-US">immobilizing</dc:subject>
	<dc:subject xml:lang="en-US">polyelectrolyte membrane</dc:subject>
	<dc:subject xml:lang="en-US">urease</dc:subject>
	<dc:description xml:lang="en-US">PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.</dc:description>
	<dc:publisher xml:lang="en-US">Directorate for Research and Innovation (DRI) ITB</dc:publisher>
	<dc:date>2019-12-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journals.itb.ac.id/index.php/jmfs/article/view/9034</dc:identifier>
	<dc:identifier>10.5614/j.math.fund.sci.2019.51.3.8</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Mathematical and Fundamental Sciences; Vol. 51 No. 3 (2019); 309-319</dc:source>
	<dc:source>2338-5510</dc:source>
	<dc:source>2337-5760</dc:source>
	<dc:source>10.5614/j.math.fund.sci.2019.51.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journals.itb.ac.id/index.php/jmfs/article/view/9034/4430</dc:relation>
</oai_dc:dc>