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Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7)

Journal of Mathematical and Fundamental Sciences · Vol. 51 No. 3 (2019) · hlm. 205–224

DOI
10.5614/j.math.fund.sci.2019.51.3.1
ISSN
2338-5510, 2337-5760
Terbit
2019-12-31
Penerbit
Directorate for Research and Innovation (DRI) ITB
Bahasa
eng

Abstrak

Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton's jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-α, IFN-γ, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression.

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  • Maranatha Christian University
  • University of Brawijaya
  • Universitas Prima Indonesia
  • University of Indonesia
  • Bandung Institute of Technology
  • Indonesian Institute of Sciences
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Rekan penulisOrganisasiArtikel bersama
Tjandrawati MozefIndonesian Institute of Sciences12
Seila ArumwardanaAfiliasi tidak diketahui10
Ermi GirsangUniversitas Prima Indonesia9
Didik PriyandokoIndonesia University of Education8
Ferry SandraUniversitas Trisakti8
Dhanar Septyawan HadiprasetyoUniversitas Jenderal Achmad Yani7
Rizal AzisUniversity of Indonesia7
Chrismis Novalinda GintingUniversitas Prima Indonesia7
I Nyoman Ehrich ListerUniversitas Prima Indonesia6
Lusiana DarsonoMaranatha Christian University6
Ahmad FariedPadjadjaran University5
Wahyu Setia WidodoAfiliasi tidak diketahui5
Teresa Liliana WargasetiaMaranatha Christian University4
The Fransiska EltaniaMaranatha Christian University4
Ika Adhani SholihahBandung Institute of Technology4
Agung NoviantoPadjadjaran University4
Arief BoedionoIPB University4
Mawar SubangkitIPB University4
Hana RatnawatiMaranatha Christian University3
Fadhilah Haifa ZahirohAfiliasi tidak diketahui3
A Aulanni’amUniversity of Brawijaya2
Chandra RisdianIndonesian Institute of Sciences2
Mohammad HidayatUniversity of Brawijaya2
Zairin NoorLambung Mangkurat University2
Agus Hadian RahimDr. Hasan Sadikin General Hospital2
Lilis Puspa FriliansariAfiliasi tidak diketahui1
Gina KhairinisaAfiliasi tidak diketahui1
Eki HerawatiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journals.itb.ac.id/index.php/jmfs/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7)</dc:title>
	<dc:creator>Widowati, Wahyu</dc:creator>
	<dc:creator>Jasaputra, Diana Krisanti</dc:creator>
	<dc:creator>Onggowidjaja, Philips</dc:creator>
	<dc:creator>Sumitro, Sutiman Bambang</dc:creator>
	<dc:creator>Widodo, Mochammad Aris</dc:creator>
	<dc:creator>Afifah, Ervi</dc:creator>
	<dc:creator>Rihibiha, Dwi Davidson</dc:creator>
	<dc:creator>Rizal, Rizal</dc:creator>
	<dc:creator>Amalia, Annisa</dc:creator>
	<dc:creator>Kusuma, Hanna Sari Widya</dc:creator>
	<dc:creator>Murti, Harry</dc:creator>
	<dc:creator>Bachtiar, Indra</dc:creator>
	<dc:subject xml:lang="en-US">breast cancer</dc:subject>
	<dc:subject xml:lang="en-US">conditioned medium</dc:subject>
	<dc:subject xml:lang="en-US">human Wharton’s jelly mesenchymal stem cells</dc:subject>
	<dc:subject xml:lang="en-US">interleukin-2</dc:subject>
	<dc:subject xml:lang="en-US">natural killer cells</dc:subject>
	<dc:description xml:lang="en-US">Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton's jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-α, IFN-γ, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression. </dc:description>
	<dc:publisher xml:lang="en-US">Directorate for Research and Innovation (DRI) ITB</dc:publisher>
	<dc:date>2019-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journals.itb.ac.id/index.php/jmfs/article/view/9081</dc:identifier>
	<dc:identifier>10.5614/j.math.fund.sci.2019.51.3.1</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Mathematical and Fundamental Sciences; Vol. 51 No. 3 (2019); 205-224</dc:source>
	<dc:source>2338-5510</dc:source>
	<dc:source>2337-5760</dc:source>
	<dc:source>10.5614/j.math.fund.sci.2019.51.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journals.itb.ac.id/index.php/jmfs/article/view/9081/3860</dc:relation>
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