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Piper crocatum Ameliorates APAP-Induced Hepatotoxicity through Antioxidant and Anti-Inflammatory Mechanisms

Journal of Mathematical and Fundamental Sciences · Vol. 54 No. 1 (2022) · hlm. 109–120

DOI
10.5614/j.math.fund.sci.2022.54.1.6
ISSN
2338-5510, 2337-5760
Terbit
2022-06-21
Penerbit
Directorate for Research and Innovation (DRI) ITB
Bahasa
eng

Abstrak

Cirrhosis is a serious hepatic disease that occurs worldwide and is caused by progressive fibrosis in the liver. Free radicals are the major cause of reactive oxygen species (ROS) generation, which affects the balance of the liver metabolism. Piper crocatum, commonly called red betel, is widely used in ethnomedicine because it has biological capabilities, including antioxidant and anti-inflammatory properties. The present study was undertaken to examine the possibility of hepaprotective activity of red betel extract on acetaminophen (APAP)-induced HepG2 cells as the cirrhosis in vitro model. Assessment of red betel extract (RBE) was performed using the colorimetric method for the quantification of LDH, AST, and ALT. As preliminary study, a cytotoxicity assay was performed at various RBE concentrations (100.00; 50.00; 25.00; 12.50; 6.25; 3.13 μg/ml) using an MTS assay to decide the safe concentration based on cell viability. The RBE treatment did not affect cell viability, even at a high concentration (100 µg/ml, p > 0.05). RBE at concentrations 25 and 100 µg/ml successfully reduced LDH, AST, and ALT activities in the hepatotoxic model in a dose-dependent manner. In the APAP-induced hepatotoxicity model, RBE positively indicated a hepatoprotective effect and cell amelioration by a decrease in hepatotoxic markers.

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  • Universitas Prima Indonesia
  • Maranatha Christian University
  • Bandung Institute of Technology
  • University of Indonesia
  • Indonesian Institute of Sciences
  • Padjadjaran University
  • Universitas Jenderal Achmad Yani
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Rekan penulisOrganisasiArtikel bersama
Hanna Sari Widya KusumaAfiliasi tidak diketahui12
Ervi AfifahAfiliasi tidak diketahui10
I Nyoman Ehrich ListerUniversitas Prima Indonesia10
Annisa AmaliaBandung Institute of Technology9
Dhanar Septyawan HadiprasetyoUniversitas Jenderal Achmad Yani6
Tjandrawati MozefIndonesian Institute of Sciences6
Didik PriyandokoIndonesia University of Education6
Teresa Liliana WargasetiaMaranatha Christian University6
Diana Krisanti JasaputraMaranatha Christian University5
Ahmad FariedPadjadjaran University5
Ali Napiah NasutionUniversitas Prima Indonesia5
Rizal AzisUniversity of Indonesia4
The Fransiska EltaniaMaranatha Christian University4
Seila ArumwardanaAfiliasi tidak diketahui4
Dwi Davidson RihibihaAfiliasi tidak diketahui4
Harry MurtiAfiliasi tidak diketahui4
Indra BachtiarAfiliasi tidak diketahui4
Hana RatnawatiMaranatha Christian University3
Sri Lestari Ramadhani NasutionUniversitas Prima Indonesia3
Lusiana DarsonoMaranatha Christian University3
Ika Adhani SholihahBandung Institute of Technology3
Philips OnggowidjajaMaranatha Christian University3
Fadhilah Haifa ZahirohAfiliasi tidak diketahui3
Ferry SandraUniversitas Trisakti3
Nadya Nazimuddin PutriUniversitas Prima Indonesia2
Tan SuyonoUniversitas Prima Indonesia2
Agung NoviantoPadjadjaran University2
Chandra RisdianIndonesian Institute of Sciences2
Putranto ManaluUniversitas Prima Indonesia2
Christin Debora BukitAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journals.itb.ac.id/index.php/jmfs/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Piper crocatum Ameliorates APAP-Induced Hepatotoxicity through Antioxidant and Anti-Inflammatory Mechanisms</dc:title>
	<dc:creator>Lister, I Nyoman Ehrich</dc:creator>
	<dc:creator>Ginting, Chrismis Novalinda</dc:creator>
	<dc:creator>Girsang, Ermi</dc:creator>
	<dc:creator>Amansyah, Adek</dc:creator>
	<dc:creator>Chiuman, Linda</dc:creator>
	<dc:creator>Yanti, Ni Luh Wisma Eka</dc:creator>
	<dc:creator>Widodo, Wahyu Setia</dc:creator>
	<dc:creator>Yusepany, Dewani Tediana</dc:creator>
	<dc:creator>Rizal, Rizal</dc:creator>
	<dc:creator>Widowati, Wahyu</dc:creator>
	<dc:subject xml:lang="en-US">aminotransferase</dc:subject>
	<dc:subject xml:lang="en-US">alanine aminotransferase</dc:subject>
	<dc:subject xml:lang="en-US">cirrhosis</dc:subject>
	<dc:subject xml:lang="en-US">lactate dehydrogenase</dc:subject>
	<dc:subject xml:lang="en-US">red betel</dc:subject>
	<dc:description xml:lang="en-US">Cirrhosis is a serious hepatic disease that occurs worldwide and is caused by progressive fibrosis in the liver. Free radicals are the major cause of reactive oxygen species (ROS) generation, which affects the balance of the liver metabolism. Piper crocatum, commonly called red betel, is widely used in ethnomedicine because it has biological capabilities, including antioxidant and anti-inflammatory properties. The present study was undertaken to examine the possibility of hepaprotective activity of red betel extract on acetaminophen (APAP)-induced HepG2 cells as the cirrhosis in vitro model. Assessment of red betel extract (RBE) was performed using the colorimetric method for the quantification of LDH, AST, and ALT. As preliminary study, a cytotoxicity  assay was performed at various RBE concentrations (100.00; 50.00; 25.00; 12.50; 6.25; 3.13 μg/ml) using an MTS assay to decide the safe concentration based on cell viability. The RBE treatment did not affect cell viability, even at a high concentration (100 µg/ml, p &amp;gt; 0.05). RBE at concentrations 25 and 100 µg/ml successfully reduced LDH, AST, and ALT activities in the hepatotoxic model in a dose-dependent manner. In the APAP-induced hepatotoxicity model, RBE positively indicated a hepatoprotective effect and cell amelioration by a decrease in hepatotoxic markers.</dc:description>
	<dc:publisher xml:lang="en-US">Directorate for Research and Innovation (DRI) ITB</dc:publisher>
	<dc:date>2022-06-21</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journals.itb.ac.id/index.php/jmfs/article/view/13499</dc:identifier>
	<dc:identifier>10.5614/j.math.fund.sci.2022.54.1.6</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Mathematical and Fundamental Sciences; Vol. 54 No. 1 (2022); 109-120</dc:source>
	<dc:source>2338-5510</dc:source>
	<dc:source>2337-5760</dc:source>
	<dc:source>10.5614/j.math.fund.sci.2022.54.1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journals.itb.ac.id/index.php/jmfs/article/view/13499/5846</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Journal of Mathematical and Fundamental Sciences</dc:rights>
</oai_dc:dc>