Akses terbuka Jurnal SINTA 1
Engineering Coffee Cherry Pulp-derived Activated Carbon for Cellulase Immobilization and its Role in Regulating Non-Sterile Coffee Fermentation
Journal of Mathematical and Fundamental Sciences · Vol. 58 No. 1 (2026) · hlm. 19–31
- ISSN
- 2338-5510, 2337-5760
- Terbit
- 2026-09-10
- Penerbit
- Directorate for Research and Innovation (DRI) ITB
- Bahasa
- eng
Abstrak
During non-sterile coffee fermentation, microbial activity and substrate availability change as the mucilage is degraded. Activated carbon prepared from Arabica coffee cherry pulp by carbonization and ZnCl₂ activation was used as a support for cellulase immobilization. Cellulase produced by Cytobacillus sp. isolated from civet (Paradoxurus hermaphroditus) feces was immobilized by adsorption and used as part of the fermentation starter system. The immobilization efficiency was 80% and the immobilized cellulase had a specific activity of 613.22 U/mg. During fermentation, cellulase activity decreased from 2.45 U/mL at 0 h to 1.71 U/mL at 72 h, with approximately 70% of the initial activity remaining. At 48 h, the reducing sugar concentration was 10.7 mg/mL in the fermentation with immobilized cellulase, compared with 6.1 mg/mL in the control. The microbial growth rate was 0.132 h⁻¹ with immobilized cellulase and 0.084 h⁻¹ in the control. Pearson correlation coefficients were 0.93 between cellulase activity and reducing sugar concentration, 0.91 between reducing sugar concentration and microbial growth, and 0.88 between cellulase activity and microbial growth rate. Immobilized cellulase was associated with changes in sugar availability and microbial growth during non-sterile coffee fermentation.
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- State University of Malang
- Universitas Islam Negeri Maulana Malik Ibrahim
- Fiji National University
- Institut Teknologi Kalimantan
- Tun Hussein Onn University of Malaysia
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| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Rudju Winarsa | Afiliasi tidak diketahui | 3 |
| Busyro Busyro | Afiliasi tidak diketahui | 2 |
| Farah Salma Elida | Afiliasi tidak diketahui | 2 |
| Ramdhan Putrasetya | Afiliasi tidak diketahui | 2 |
| Hidayat Teguh Wiyono | Afiliasi tidak diketahui | 2 |
| Agus Mukti Wibowo | State University of Malang | 1 |
| Sugeng Utaya | State University of Malang | 1 |
| W. Wahjoedi | State University of Malang | 1 |
| Siti Zubaidah | State University of Malang | 1 |
| Ravinesh Rohit Prasad | Fiji National University | 1 |
| Sumarmi Sumarmi | State University of Malang | 1 |
| Syamsul Bachri | State University of Malang | 1 |
| Singgih Susilo | State University of Malang | 1 |
| Ramadhani Lausi Mkumbachi | Afiliasi tidak diketahui | 1 |
| Ahmad Ghozi | Universitas Islam Negeri Maulana Malik Ibrahim | 1 |
| N. Teti | Afiliasi tidak diketahui | 1 |
| I. Hernaman | Afiliasi tidak diketahui | 1 |
| B. Ayuningsih | Afiliasi tidak diketahui | 1 |
| D. Ramdani | Afiliasi tidak diketahui | 1 |
| Nanda Widaninggar | Afiliasi tidak diketahui | 1 |
| Much Aziz Muslim | Tun Hussein Onn University of Malaysia | 1 |
| Alamsyah Alamsyah | Institut Teknologi Kalimantan | 1 |
| Muhamad Zulfatul A'la | Afiliasi tidak diketahui | 1 |
| Rahmawati L. Maghfiroh | Afiliasi tidak diketahui | 1 |
| Dea Amelia | Afiliasi tidak diketahui | 1 |
| Anisah Mega Andini | Afiliasi tidak diketahui | 1 |
| Aisyah Deri Ayu Tungga Safitri | Afiliasi tidak diketahui | 1 |
| Yuant Tiandho | Afiliasi tidak diketahui | 1 |
| Rosita Fitrah Dewi | Afiliasi tidak diketahui | 1 |
| Arsal Arsal | Afiliasi tidak diketahui | 1 |
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