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Acute and Lethal Dose Toxicity Test of Pure Eel Fish (Anguilla marmorata [Q.] Gaimard) Oil in Mice (Mus musculus)

Journal of Public Health and Pharmacy · Vol. 5 No. 3 (2025) · hlm. 664–677

DOI
10.56338/jphp.v5i3.5987
ISSN
2775-4952
Terbit
2025-11-03
Penerbit
Pusat Pengembangan Teknologi Informasi dan Jurnal Universitas Muhammadiyah Palu
Bahasa
eng

Abstrak

Introduction: Eel fish oil (Anguilla marmorata (Q.) Gaimard) highlighted its nutritional advantages, especially vitamin A, EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid), and omega-3. Unsaturated fats in fish oil are known to have positive effects in the treatment of various diseases, although the potential negative impacts also need to be considered. Methods: This research aims to focus on the cytotoxic activity of eel fish oil and carry out extraction using the soxhlation method, Thomson and Weil, and Metode fixed dose. To evaluate the toxicity of the extract, the acute toxicity test was carried out by measuring the middle lethal dose (LD50), which is often used as a benchmark. Apart from that, the research also aims to determine the lethal dose 50 (LD??) value of eel fish oil, namely the dose that can cause the death of 50% of the test animal population. The extraction process uses diethyl ether solvent at 60°C, followed by purification using a 3% bentonite adsorbent. The test was carried out twice with female mice and rats as test animals, divided into groups with oral doses of 5, 50, 300, 2000, and 5000 mg/kg. Result: The results showed no deaths in the test animals, and the LD50 of eel fish oil extract was greater than the dose used. Clinical symptoms in mice include decreased motor activity and increased grooming frequency as the dose increases, as well as increased muscle tone and tension responses. Conclusion: Based on the research results, it can be concluded that eel fish oil extracts up to a dose of 5000 mg/kg are still classified as "safe" for use because they do not cause death in test animals.

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  • Tadulako University
  • University of Mataram
  • Ministry of Marine Affairs and Fisheries
  • Hasanuddin University
  • Universitas Muhammadiyah Palu
  • Universitas Methodist Indonesia
  • Universitas Muhammadiyah Luwuk Banggai
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Rekan penulisOrganisasiArtikel bersama
Yusriadi YusriadiHasanuddin University2
Finta AmalindaUniversitas Muhammadiyah Palu2
Ihwan IhwanTadulako University2
Andi Ratihsekarningrum SiradjeTadulako University2
Apriyana SoputraTadulako University2
Novita Christiana PongananTadulako University2
Klerensia Defneir TitingTadulako University2
Novianti BattiTadulako University2
Andi MustikaTadulako University2
Andi AlfiantoTadulako University2
Moh. RifkiTadulako University2
Janes KrislyTadulako University2
Rimbun SiringoringoUniversitas Methodist Indonesia1
Abdul Wahab JufriUniversity of Mataram1
Agus RamdaniUniversity of Mataram1
Muhammad SafirTadulako University1
Kasim MansyurTadulako University1
Fadly Yasin TantuTadulako University1
Andi Iqbal BurhanuddinHasanuddin University1
Abdul GaniUniversitas Muhammadiyah Luwuk Banggai1
Achmad Afif BakriMinistry of Marine Affairs and Fisheries1
Devita Tetra AdrianyMinistry of Marine Affairs and Fisheries1
Muh. HerjayantoTadulako University1
Samliok NdobeTadulako University1
Nurjirana NurjiranaNational Research and Innovation Agency1
Lady Diana KhartionoAfiliasi tidak diketahui1
Alimudin LaapoIPB University1
Andi Darmawati TombolotutuUniversitas Muhammadiyah Palu1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.unismuhpalu.ac.id/index.php/jphp/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Acute and Lethal Dose Toxicity Test of Pure Eel Fish (Anguilla marmorata [Q.] Gaimard) Oil in Mice (Mus musculus)</dc:title>
	<dc:creator>Jamaluddin, Jamaluddin</dc:creator>
	<dc:creator>Ihwan, Ihwan</dc:creator>
	<dc:creator>Khaerati, Khildah </dc:creator>
	<dc:creator>Hasanuddin, Asriani </dc:creator>
	<dc:creator>Serdiati, Novalina </dc:creator>
	<dc:creator>Nilawati, Jusri </dc:creator>
	<dc:creator>Wijaya, Berniawan </dc:creator>
	<dc:creator>Yuliet, Yuliet</dc:creator>
	<dc:creator>Ahmad, Iftitah </dc:creator>
	<dc:subject xml:lang="en-US">Anguilla marmorata</dc:subject>
	<dc:subject xml:lang="en-US">Mus musculus</dc:subject>
	<dc:subject xml:lang="en-US">Acute Toxicity</dc:subject>
	<dc:subject xml:lang="en-US">Lethal Dose 50</dc:subject>
	<dc:description xml:lang="en-US">Introduction: Eel fish oil (Anguilla marmorata (Q.) Gaimard) highlighted its nutritional advantages, especially vitamin A, EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid), and omega-3. Unsaturated fats in fish oil are known to have positive effects in the treatment of various diseases, although the potential negative impacts also need to be considered.
Methods: This research aims to focus on the cytotoxic activity of eel fish oil and carry out extraction using the soxhlation method, Thomson and Weil, and Metode fixed dose. To evaluate the toxicity of the extract, the acute toxicity test was carried out by measuring the middle lethal dose (LD50), which is often used as a benchmark. Apart from that, the research also aims to determine the lethal dose 50 (LD??) value of eel fish oil, namely the dose that can cause the death of 50% of the test animal population. The extraction process uses diethyl ether solvent at 60°C, followed by purification using a 3% bentonite adsorbent. The test was carried out twice with female mice and rats as test animals, divided into groups with oral doses of 5, 50, 300, 2000, and 5000 mg/kg.
Result: The results showed no deaths in the test animals, and the LD50 of eel fish oil extract was greater than the dose used. Clinical symptoms in mice include decreased motor activity and increased grooming frequency as the dose increases, as well as increased muscle tone and tension responses.
Conclusion: Based on the research results, it can be concluded that eel fish oil extracts up to a dose of 5000 mg/kg are still classified as "safe" for use because they do not cause death in test animals.</dc:description>
	<dc:publisher xml:lang="en-US">Pusat Pengembangan Teknologi Informasi dan Jurnal Universitas Muhammadiyah Palu</dc:publisher>
	<dc:date>2025-11-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.unismuhpalu.ac.id/index.php/jphp/article/view/5987</dc:identifier>
	<dc:identifier>10.56338/jphp.v5i3.5987</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Public Health and Pharmacy; Vol. 5 No. 3 (2025); 664-677</dc:source>
	<dc:source>2775-4952</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jurnal.unismuhpalu.ac.id/index.php/jphp/article/view/5987/6219</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Journal of Public Health and Pharmacy</dc:rights>
</oai_dc:dc>