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Pathogenic Bacteria on Musca domestica: Identification and Antibiotic Sensitivity via MALDI-TOF

Journal of Public Health and Pharmacy · Vol. 5 No. 1 (2025) · hlm. 29–41

DOI
10.56338/jphp.v5i1.6268
ISSN
2775-4952
Terbit
2025-02-10
Penerbit
Pusat Pengembangan Teknologi Informasi dan Jurnal Universitas Muhammadiyah Palu
Bahasa
eng

Abstrak

Introduction: Flies are mechanical vectors that can transmit a variety of pathogenic bacteria, potentially causing human infections, especially in hospital settings. Rapid and accurate identification of these pathogenic bacteria, as well as assessment of their sensitivity to antibiotics, is essential for effective infection control. Methods: Sampling of flies in temporary rubbish bins in the hospital environment. Isolation of pathogenic bacteria from fly body surface by culture method, identification of bacteria using MALDI-TOF Mass Spectrometry technology. Antibiotic sensitivity test was conducted using Viteks 2 Compact to assess the effectiveness of therapy against the isolated bacteria. Ethical approval was obtained from Hasanuddin University Makassar. Results: Four species of pathogenic bacteria, namely Staphylococcus sciuri, Staphylococcus xylosus, Klebsiella pneumoniae, and Proteus mirabilis, were successfully identified quickly and accurately using the MALDI-TOF Mass Spectrometry method. Staphylococcus sciuri and Staphylococcus xylosus are sensitive to Benzylpenicillin, Oxacillin, and Ciprofloxacin, although Staphylococcus sciuri shows moderate resistance to Moxifloxacin. Klebsiella pneumoniae is resistant to Ampicillin, but is effective when combined with Sulbactam, while Proteus mirabilis is generally sensitive, except for Tigecycline. These findings are relevant for infection prevention strategies in hospitals, providing more appropriate antibiotic use guidance and supporting antimicrobial resistance control programs. Conclusion: MALDI-TOF Mass Spectrometry method proved effective in the rapid and accurate identification of pathogenic bacteria from flies. Antibiotic sensitivity testing is essential to determine the most effective therapy and avoid the use of less effective antibiotics.

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  • Universitas Tompotika Luwuk
  • Muhammadiyah University of Makassar
  • Universitas Airlangga
  • Hasanuddin University
  • Universitas Muhammadiyah Parepare
  • Universitas PGRI Palembang
  • Universitas Islam Negeri Sultan Syarif Kasim Riau
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Rekan penulisOrganisasiArtikel bersama
Anwar MallongiHasanuddin University2
Nanda SafiraMuhammadiyah University of Makassar1
Rohana RohanaUniversitas PGRI Palembang1
Syamsia SyamsiaMuhammadiyah University of Makassar1
Andi Rahayu AnwarMuhammadiyah University of Makassar1
Iradhatullah RahimUniversitas Muhammadiyah Parepare1
Selfi AmandiraMuhammadiyah University of Makassar1
Ilhamsyah AidinMuhammadiyah University of Makassar1
Sukma SyafarMuhammadiyah University of Makassar1
Mimi HariyaniUniversitas Islam Negeri Sultan Syarif Kasim Riau1
Rahel Wahjuni SutjiptoUniversitas Airlangga1
Satiti KuntariUniversitas Airlangga1
Yemy AmelianaUniversitas Airlangga1
Seno PradopoUniversitas Airlangga1
Ramli BidullahAfiliasi tidak diketahui1
Sandy N. SakatiAfiliasi tidak diketahui1
Dwi Wahyu BalebuUniversitas Tompotika Luwuk1
Herawati HerawatiUniversitas Tompotika Luwuk1
A. Arsunan ArsinAfiliasi tidak diketahui1
Hasanuddin IshakHasanuddin University1
Agus Bintara BirawidaHasanuddin University1
Ida Leida MariaHasanuddin University1
Bambang DwicahyaUniversitas Tompotika Luwuk1
Firdaus HamidHasanuddin University1
Muhammad SyahrirUniversitas Tompotika Luwuk1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.unismuhpalu.ac.id/index.php/jphp/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Pathogenic Bacteria on Musca domestica: Identification and Antibiotic Sensitivity via MALDI-TOF</dc:title>
	<dc:creator>Kanan, Maria</dc:creator>
	<dc:creator>Herawati Herawati</dc:creator>
	<dc:creator>Inda Hafid</dc:creator>
	<dc:creator>Sandy N. Sakati</dc:creator>
	<dc:creator>Muhammad Syahrir</dc:creator>
	<dc:subject xml:lang="en-US">MALDI-TOF</dc:subject>
	<dc:subject xml:lang="en-US">Mass Spectrometry</dc:subject>
	<dc:subject xml:lang="en-US">Pathogenic Bacteria</dc:subject>
	<dc:subject xml:lang="en-US">Antibiotic Sensitivity</dc:subject>
	<dc:subject xml:lang="en-US">Flies</dc:subject>
	<dc:description xml:lang="en-US">Introduction: Flies are mechanical vectors that can transmit a variety of pathogenic bacteria, potentially causing human infections, especially in hospital settings. Rapid and accurate identification of these pathogenic bacteria, as well as assessment of their sensitivity to antibiotics, is essential for effective infection control.
Methods: Sampling of flies in temporary rubbish bins in the hospital environment. Isolation of pathogenic bacteria from fly body surface by culture method, identification of bacteria using MALDI-TOF Mass Spectrometry technology. Antibiotic sensitivity test was conducted using Viteks 2 Compact to assess the effectiveness of therapy against the isolated bacteria. Ethical approval was obtained from Hasanuddin University Makassar.
Results: Four species of pathogenic bacteria, namely Staphylococcus sciuri, Staphylococcus xylosus, Klebsiella pneumoniae, and Proteus mirabilis, were successfully identified quickly and accurately using the MALDI-TOF Mass Spectrometry method. Staphylococcus sciuri and Staphylococcus xylosus are sensitive to Benzylpenicillin, Oxacillin, and Ciprofloxacin, although Staphylococcus sciuri shows moderate resistance to Moxifloxacin. Klebsiella pneumoniae is resistant to Ampicillin, but is effective when combined with Sulbactam, while Proteus mirabilis is generally sensitive, except for Tigecycline. These findings are relevant for infection prevention strategies in hospitals, providing more appropriate antibiotic use guidance and supporting antimicrobial resistance control programs.
Conclusion: MALDI-TOF Mass Spectrometry method proved effective in the rapid and accurate identification of pathogenic bacteria from flies. Antibiotic sensitivity testing is essential to determine the most effective therapy and avoid the use of less effective antibiotics.</dc:description>
	<dc:publisher xml:lang="en-US">Pusat Pengembangan Teknologi Informasi dan Jurnal Universitas Muhammadiyah Palu</dc:publisher>
	<dc:date>2025-02-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.unismuhpalu.ac.id/index.php/jphp/article/view/6268</dc:identifier>
	<dc:identifier>10.56338/jphp.v5i1.6268</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Public Health and Pharmacy; Vol. 5 No. 1 (2025); 29-41</dc:source>
	<dc:source>2775-4952</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jurnal.unismuhpalu.ac.id/index.php/jphp/article/view/6268/5009</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Journal of Public Health and Pharmacy</dc:rights>
</oai_dc:dc>