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Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro

Journal of Tropical Biodiversity and Biotechnology · Vol. 3 No. 3 (2018) · hlm. 73–79

DOI
10.22146/jtbb.33441
ISSN
2540-9581, 2540-9573
Terbit
2018-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. Macrophage cells separated form peritoneal fluid used RPMI medium. Phagocytosis activity and phagocytosis capacity of macrophages were performed in vitro using latex beads that suspended in phosphate buffered saline (PBS). The antioxidant activity was measured by spectrophotometry technique with 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution. All treatments were done three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytosis activity followed by chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetate extract. The ethyl acetate extract was also the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract has a high immunomodulatory activity and antioxidant activity which contained phenolics, flavonoids, tannins, and terpenoids.

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  • Universitas Gadjah Mada
  • Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales
  • King Saud University
  • ABSys
  • National Research and Innovation Agency
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Rekan penulisOrganisasiArtikel bersama
Subagus WahyuonoUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Adyatma Irawan SantosaUniversitas Gadjah Mada1
Adenisa Hanifah IrbatiUniversitas Gadjah Mada1
Belinda WinonaUniversitas Gadjah Mada1
Reko Saputra JayaUniversitas Gadjah Mada1
Annisa Lisa AndriyaniUniversitas Gadjah Mada1
Chilya Qurrota A'yunUniversitas Gadjah Mada1
Abdulrahman S. AldawoodKing Saud University1
Siti Shofa Assyifa’ul Qulbi BaridUniversitas Gadjah Mada1
Fathur Syahrian RamadhaniUniversitas Gadjah Mada1
Hari PurwantoUniversitas Gadjah Mada1
Hendry T.S.S.G. SaragihUniversitas Gadjah Mada1
SukirnoUniversitas Gadjah Mada1
Siti SubandiyahUniversitas Gadjah Mada1
Junaidi JunaidiAmélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales1
Anne Clément‐VidalAmélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales1
Éric GohetABSys1
Pascal MontoroAmélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales1
Sukarti MoeljopawiroUniversitas Gadjah Mada1
Yoga Mulia PratamaUniversitas Gadjah Mada1
G. GalihUniversitas Gadjah Mada1
Lisna HidayatiUniversitas Gadjah Mada1
Dyah IsmoyowatiUniversitas Gadjah Mada1
Dio N. WijayaUniversitas Gadjah Mada1
Febri Adi SusantoUniversitas Gadjah Mada1
Donny WidiantoUniversitas Gadjah Mada1
Charlie Ester de FretesNational Research and Innovation Agency1
Nastiti WijayantiUniversitas Gadjah Mada1
Dian Ayuning TyasUniversitas Gadjah Mada1
Purnomo PurnomoUniversitas Gadjah Mada1
Sigit Dwi MaryantoUniversitas Gadjah Mada1
Randi Abdur RohmanAfiliasi tidak diketahui1
Roberdi RoberdiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro</dc:title>
	<dc:creator>Mahadi, Rendi</dc:creator>
	<dc:creator>Rasyiid, Mustafid</dc:creator>
	<dc:creator>Dharma, Krisnanda Surya</dc:creator>
	<dc:creator>Anggraini, Lindia</dc:creator>
	<dc:creator>Nurdiyanti, Rahma</dc:creator>
	<dc:creator>Nuringtyas, Tri Rini</dc:creator>
	<dc:subject xml:lang="en-US">Cyclea barbata Miers.; green grass jelly; immunomodulator; antioxidant; DPPH</dc:subject>
	<dc:description xml:lang="en-US">Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. Macrophage cells separated form peritoneal fluid used RPMI medium. Phagocytosis activity and phagocytosis capacity of macrophages were performed in vitro using latex beads that suspended in phosphate buffered saline (PBS). The antioxidant activity was measured by spectrophotometry technique with 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution. All treatments were done three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytosis activity followed by chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetate extract. The ethyl acetate extract was also the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract has a high immunomodulatory activity and antioxidant activity which contained phenolics, flavonoids, tannins, and terpenoids.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2018-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/33441</dc:identifier>
	<dc:identifier>10.22146/jtbb.33441</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 3, No 3 (2018): December; 73-79</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/33441/23384</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/33441/3933</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>