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Composition of Mitochondrial DNA 16S Nucleotide of Dwarf Snakehead (Channa gachua Hamilton, 1822) from Keji River, Magelang, Central Java

Journal of Tropical Biodiversity and Biotechnology · Vol. 3 No. 2 (2018) · hlm. 57–61

DOI
10.22146/jtbb.35613
ISSN
2540-9581, 2540-9573
Terbit
2018-09-25
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Indonesia has a high marine and freshwater biodiversity including freshwater fish biodiversity. One of freshwater fish which is commonly consumed by Indonesian people is dwarf snakehead (Channa gachua Hamilton, 1822). However, research on genetic characterization, especially the composition of mtDNA 16S nucleotide of dwarf snakehead has poorly understood. Therefore, the aim of this study was to determine the composition of mtDNA 16S nucleotide of dwarf snakehead as a part of genetic characterization of the fish species taken from Keji River, Magelang, Central Java which has not been previously examined. This study analyzed 16S mt-DNA of two samples of dwarf snakehead from Keji River (KTS-01 and KTS-02). In addition, two sequences of Channa gachua with accession number KU986900, KU238074, and HM117234-HM117238 taken from GenBank were used as a comparison. A method used in this research was a PCR method and primers used in this research were 16Sar and 16Sbr. The results revealed that the average of nucleotide composition T, C, A and G of the fish species was 23.04%, 25.13%, 29.06% and 22.77% respectively whereas the average rate of nucleotide composition A+ T and G+ C was 52.10% and 47.90% respectively. The two dwarf snakehead had similar T and C composition but different in A and G composition. In addition, the G+C content in KTS-01 and KTS-02 had the highest frequency compared to other dwarf snakehead taken from GenBank. From this finding it could be assumed that there is genetic variation between the two dwarf snakehead from Keji River which is important genetic data for breeding program of the fish species in the future.

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Politeknik Bina Husada Kendari
  • Universitas Airlangga
  • University of Bengkulu
  • Kyoto University
  • Tadulako University
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Rekan penulisOrganisasiArtikel bersama
Katon Waskito AjiUniversitas Gadjah Mada5
Dwi Sendi PriyonoUniversitas Gadjah Mada3
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Rury EprilurahmanUniversitas Gadjah Mada2
Zuliyati RohmahUniversitas Gadjah Mada2
Lukman HakimUniversitas Gadjah Mada2
Febrina Amaliya Rha’ifaUniversitas Gadjah Mada2
Amir HamidyIndonesian Institute of Sciences2
Rosichon UbaidillahIndonesian Institute of Sciences2
Diana FebriyantiUniversitas Gadjah Mada2
Karmilah KarmilahPoliteknik Bina Husada Kendari1
Anggun Diyan NurhasanahUniversitas Gadjah Mada1
Madyan Akmal HidayatUniversitas Gadjah Mada1
Muslifah HasanahUniversitas Gadjah Mada1
Ganies Riza AristyaUniversitas Gadjah Mada1
Adib Fakhruddin YusufUniversitas Gadjah Mada1
Adristi Shafa WidyasariUniversitas Gadjah Mada1
Niken Satuti Nur HandayaniUniversitas Gadjah Mada1
Ananto Puradi NainggolanUniversitas Gadjah Mada1
Vida Rahma LatifahUniversitas Gadjah Mada1
Vivi Indah NurcahyatiUniversitas Gadjah Mada1
Aldy Riau Wansyah HasibuanUniversitas Gadjah Mada1
Arini Dian PratiwiUniversitas Gadjah Mada1
Rindu AurantikaUniversitas Gadjah Mada1
Indra LesmanaUniversitas Gadjah Mada1
Fanni KhoirillahUniversitas Gadjah Mada1
Tasya NuraliyahUniversitas Gadjah Mada1
Vindi DwijayantiUniversitas Gadjah Mada1
Anjar Tri WibowoUniversitas Airlangga1
Rika Lathif HasanUniversitas Gadjah Mada1
Khadija Lung AyuUniversitas Gadjah Mada1
Nofita RatmanUniversitas Gadjah Mada1
Deiandra Jasmine AudreaUniversitas Gadjah Mada1
Vestidhia Yunisya AtmajaUniversity of Bengkulu1
Misbahul MunirKyoto University1
Tomi KasayevUniversitas Gadjah Mada1
Muhammad Zulfiqar Meizar PratamaUniversitas Gadjah Mada1
Manap TriantoTadulako University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Composition of Mitochondrial DNA 16S Nucleotide of Dwarf Snakehead  (Channa gachua Hamilton, 1822) from Keji River, Magelang, Central Java</dc:title>
	<dc:creator>Ilmi, Warisatul</dc:creator>
	<dc:creator>Arisuryanti, Tuty</dc:creator>
	<dc:subject xml:lang="en-US">dwarf snakehead; genetic characterization; mtDNA 16S; nucleotide composition</dc:subject>
	<dc:description xml:lang="en-US">Indonesia has a high marine and freshwater biodiversity including freshwater fish biodiversity. One of freshwater fish which is commonly consumed by Indonesian people is dwarf snakehead (Channa gachua Hamilton, 1822). However, research on genetic characterization, especially the composition of mtDNA 16S nucleotide of dwarf snakehead has poorly understood. Therefore, the aim of this study was to determine the composition of mtDNA 16S nucleotide of dwarf snakehead as a part of genetic characterization of the fish species taken from Keji River, Magelang, Central Java which has not been previously examined. This study analyzed 16S mt-DNA of two samples of dwarf snakehead from Keji River (KTS-01 and KTS-02). In addition, two sequences of Channa gachua with accession number KU986900, KU238074, and HM117234-HM117238 taken from GenBank were used as a comparison. A method used in this research was a PCR method and primers used in this research were 16Sar and 16Sbr. The results revealed that the average of nucleotide composition T, C, A and G of the fish species was 23.04%, 25.13%, 29.06% and 22.77% respectively whereas the average rate of nucleotide composition A+ T and G+ C was 52.10% and 47.90% respectively. The two dwarf snakehead had similar T and C composition but different in A and G composition. In addition, the G+C content in KTS-01 and KTS-02 had the highest frequency compared to other dwarf snakehead taken from GenBank. From this finding it could be assumed that there is genetic variation between the two dwarf snakehead from Keji River which is important genetic data for breeding program of the fish species in the future.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2018-09-25</dc:date>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/35613</dc:identifier>
	<dc:identifier>10.22146/jtbb.35613</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 3, No 2 (2018): August; 57-61</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/35613/22084</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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