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Genetic Identification of Freshwater Fish Species Through DNA Barcoding from Lake Lebo Taliwang, West Nusa Tenggara

Journal of Tropical Biodiversity and Biotechnology · Vol. 4 No. 3 (2019) · hlm. 107–112

DOI
10.22146/jtbb.46256
ISSN
2540-9581, 2540-9573
Terbit
2019-12-16
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.

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  • Universitas Gadjah Mada
  • Universitas Islam Negeri Ar-Raniry
  • Indonesian Institute of Sciences
  • Institut Teknologi Sepuluh Nopember
  • UIN Sunan Ampel Surabaya
  • Indonesia Open University
  • Universitas Syiah Kuala
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Rekan penulisOrganisasiArtikel bersama
Katon Waskito AjiUniversitas Gadjah Mada5
Dwi Sendi PriyonoUniversitas Gadjah Mada3
Febrina Amaliya Rha’ifaUniversitas Gadjah Mada3
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Rury EprilurahmanUniversitas Gadjah Mada2
Zuliyati RohmahUniversitas Gadjah Mada2
Amir HamidyIndonesian Institute of Sciences2
Rosichon UbaidillahIndonesian Institute of Sciences2
Diana FebriyantiUniversitas Gadjah Mada2
Chastine FatichahInstitut Teknologi Sepuluh Nopember1
Siti RochimahInstitut Teknologi Sepuluh Nopember1
KusnadiIndonesia Open University1
I Nengah Surati JayaAfiliasi tidak diketahui1
Nining PuspaningsihAfiliasi tidak diketahui1
Makin BasukiAfiliasi tidak diketahui1
Muji BurrahmadAfiliasi tidak diketahui1
Edy MarsudiAfiliasi tidak diketahui1
Muji BurrahmadUniversitas Syiah Kuala1
Edy MarsudiAfiliasi tidak diketahui1
Lukman Nuzul HakimNational Research and Innovation Agency1
Subyantoro SubyantoroAfiliasi tidak diketahui1
Bakhtiar BakhtiarAfiliasi tidak diketahui1
Erita HayatiAfiliasi tidak diketahui1
Sabaruddin ZakariaAfiliasi tidak diketahui1
Happy SaputraUniversitas Islam Negeri Ar-Raniry1
Sapuan HusniUniversitas Islam Negeri Ar-Raniry1
Ali Sajjad BaalyUIN Sunan Ampel Surabaya1
Abu Bakar YamaniUIN Sunan Ampel Surabaya1
Khairani KhairaniUniversitas Islam Negeri Ar-Raniry1
Khairuddin HasballahAfiliasi tidak diketahui1
Sayuthi M. AminAfiliasi tidak diketahui1
Riza Afrian MustaqiemAfiliasi tidak diketahui1
Andi RahmanAfiliasi tidak diketahui1
Murodi MurodiAfiliasi tidak diketahui1
Adib Fakhruddin YusufUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Genetic Identification of Freshwater Fish Species Through DNA Barcoding from Lake Lebo Taliwang, West Nusa Tenggara</dc:title>
	<dc:creator>Arisuryanti, Tuty</dc:creator>
	<dc:creator>Hasan, Rika Lathif</dc:creator>
	<dc:creator>Ayu, Khadija Lung</dc:creator>
	<dc:creator>Ratman, Nofita</dc:creator>
	<dc:creator>Hakim, Lukman</dc:creator>
	<dc:subject xml:lang="en-US">genetic identification; freshwater fish; Lake Lebo Taliwang; DNA barcoding</dc:subject>
	<dc:description xml:lang="en-US">Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2019-12-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/46256</dc:identifier>
	<dc:identifier>10.22146/jtbb.46256</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 4, No 3 (2019): Desember; 107-112</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/46256/26468</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/46256/8198</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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