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RAPD Analysis for Genetic Variability Detection of Mutant Soybean (Glycine max (L.) Merr)

Journal of Tropical Biodiversity and Biotechnology · Vol. 5 No. 1 (2020) · hlm. 68–77

DOI
10.22146/jtbb.53653
ISSN
2540-9581, 2540-9573
Terbit
2020-04-14
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program.

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  • Indonesian Institute of Sciences
  • National Research and Innovation Agency
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • University of Brawijaya
  • Khairun University
  • Jenderal Soedirman University
  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
Shofiyatul Mas’udahIndonesian Institute of Sciences3
Setyawan Agung DanartoIndonesian Institute of Sciences2
Janis DamaiyaniIndonesian Institute of Sciences2
Siti NurfadilahIndonesian Institute of Sciences2
Ruri Siti ResmisariUniversitas Islam Negeri Maulana Malik Ibrahim2
Elok Rifqi FirdianaNational Research and Innovation Agency2
Dewi LestariNational Research and Innovation Agency2
Rasyadan Taufiq ProbojatiState University of Malang2
TrimantoAfiliasi tidak diketahui1
Sugeng BudihartaIndonesian Institute of Sciences1
TITUT YULISTYARINIIndonesian Institute of Sciences1
Rachmawan Adi LaksonoIndonesian Institute of Sciences1
Nurlaily LaviantiAfiliasi tidak diketahui1
Bekti YunantoAfiliasi tidak diketahui1
Trimanto TrimantoAfiliasi tidak diketahui1
Fauziah FauziahIndonesian Institute of Sciences1
Dewi Ayu LestariIndonesian Institute of Sciences1
Suyono SuyonoUniversitas Islam Negeri Maulana Malik Ibrahim1
Bagas PratesoUniversitas Islam Negeri Maulana Malik Ibrahim1
Eriyanto YusnawanNational Research and Innovation Agency1
Ridesti RindyastutiIndonesian Institute of Sciences1
Ilham Kurnia AbywijayaIndonesian Institute of Sciences1
Ruth Feti RahayuniatiJenderal Soedirman University1
Siti SubandiyahUniversitas Gadjah Mada1
Shofiyatul MasudahAfiliasi tidak diketahui1
Gebby Agnessya Esa OktaviaIndonesian Institute of Sciences1
Kurniawan BudiartoNational Research and Innovation Agency1
Ahmad Syahrian SiregarNational Research and Innovation Agency1
Elga RenjanaNational Research and Innovation Agency1
Linda Wige NingrumNational Research and Innovation Agency1
Trimanto TrimantoIndonesian Institute of Sciences1
Apriyono RahadiantoroNational Research and Innovation Agency1
Suyono SuyonoUniversitas Islam Negeri Maulana Malik Ibrahim1
Imey Tamara IndiviaUniversitas Islam Negeri Maulana Malik Ibrahim1
Fitriyah FitriyahUniversitas Islam Negeri Maulana Malik Ibrahim1
Rodiyati AzrianingsihUniversity of Brawijaya1
Isna Arofatun NikmahUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">RAPD Analysis for Genetic Variability Detection of Mutant Soybean (Glycine max (L.) Merr)</dc:title>
	<dc:creator>Wahyudi, Didik</dc:creator>
	<dc:creator>Hapsari, Lia</dc:creator>
	<dc:creator>Sundari, S.</dc:creator>
	<dc:subject xml:lang="en-US">ethyl methane sulphonate; genetic mutation; RAPD; soybean</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-04-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/53653</dc:identifier>
	<dc:identifier>10.22146/jtbb.53653</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 5, No 1 (2020): April; 68-77</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/53653/27415</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/53653/10907</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/53653/10908</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>