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Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’

Journal of Tropical Biodiversity and Biotechnology · Vol. 5 No. 2 (2020) · hlm. 124–131

DOI
10.22146/jtbb.53677
ISSN
2540-9581, 2540-9573
Terbit
2020-08-15
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days, produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC, and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.

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  • Universitas Gadjah Mada
  • Universitas Pattimura
  • University of Warwick
  • Diponegoro University
  • Universitas Muhammadiyah Semarang
  • Jenderal Soedirman University
  • State University of Semarang
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Aziz PurwantoroUniversitas Gadjah Mada14
Sukarti MoeljopawiroUniversitas Gadjah Mada6
Muhammad Dylan LawrieUniversitas Gadjah Mada5
Windi MoseUniversitas Pattimura4
Aries Bagus SasongkoUniversitas Gadjah Mada4
Febri Yuda KurniawanUniversitas Gadjah Mada3
Jose Gutierrez‐MarcosUniversity of Warwick3
Nintya SetiariDiponegoro University2
Jaka WidadaUniversitas Gadjah Mada2
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Sapto IndriokoUniversitas Gadjah Mada2
Mohammad Na’iemUniversitas Gadjah Mada2
Exsyupransia MursyantiUniversitas Gadjah Mada2
Fitriana PuspitasariUniversitas Gadjah Mada2
Naufal Ghozi Aditya PerdanaUniversitas Gadjah Mada2
Dika SundariUniversitas Gadjah Mada2
Zulfa LayinaUniversitas Gadjah Mada2
Della Rosiana NingtiasUniversitas Gadjah Mada2
Falah Nur AlifiantoUniversitas Gadjah Mada2
Suharyanto SuharyantoUniversitas Gadjah Mada1
Utaminingsih UtaminingsihUniversitas Gadjah Mada1
Sulhan EtfantiUniversitas Gadjah Mada1
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1
Dwi Umi SiswantiUniversitas Gadjah Mada1
Fitria Yulia RachmawatiUniversitas Gadjah Mada1
Aprida NikenUniversitas Gadjah Mada1
Rega Virgiyana AgustinUniversitas Gadjah Mada1
Niken WulansariUniversitas Gadjah Mada1
Rina ArimarsetiowatiIndonesian Coffee and Cocoa Research Institute1
Yohana Theresia Maria AstutiInstitut Pertanian Stiper1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’</dc:title>
	<dc:creator>Pagalla, Devi Bunga</dc:creator>
	<dc:creator>Indrianto, Ari</dc:creator>
	<dc:creator>Maryani, Maryani</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US">Biology</dc:subject>
	<dc:subject xml:lang="en-US">Eggplant; flower bud; microspore; stress treatment; embryogenic microspore</dc:subject>
	<dc:description xml:lang="en-US">The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days,  produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC,  and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">This project was supported by funding from the Hibah Penelitian Kolaborasi Dosen dan Mahasiswa” Faculty of Biology, Universitas Gadjah Mada 2019</dc:contributor>
	<dc:date>2020-08-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/53677</dc:identifier>
	<dc:identifier>10.22146/jtbb.53677</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 5, No 2 (2020): August; 124-131</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/53677/28389</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/53677/11117</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/53677/11118</dc:relation>
	<dc:coverage xml:lang="en-US">South East Asia; Indonesia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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