Callus induction and secondary metabolite profile from Elephantopus scaber L.

Junairiah, Junairiah; Wulandari, Diah Ayu; Utami, Edy Setiti Wida; Zuraidassanaaz, Nabilah Istighfari

doi:10.22146/jtbb.59234

Akses terbuka Jurnal SINTA 1

Callus induction and secondary metabolite profile from Elephantopus scaber L.

Journal of Tropical Biodiversity and Biotechnology · Vol. 6 No. 1 (2021)

DOI: 10.22146/jtbb.59234
ISSN: 2540-9581, 2540-9573
Terbit: 2021-03-01
Penerbit: Universitas Gadjah Mada
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-sa/4.0

Abstrak

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol. Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.

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Universitas Airlangga
Indonesian Institute of Sciences
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Rekan penulis	Organisasi	Artikel bersama
Imam Baehaqie	Afiliasi tidak diketahui	1
Gebby Agnessya Esa Oktavia	Indonesian Institute of Sciences	1
Yosephine Sri Wulan Manuhara	Universitas Airlangga	1
Hery Purnobasuki	Universitas Airlangga	1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en-US">Callus induction and secondary metabolite profile from Elephantopus scaber L.</dc:title>
	<dc:creator>Junairiah, Junairiah</dc:creator>
	<dc:creator>Wulandari, Diah Ayu</dc:creator>
	<dc:creator>Utami, Edy Setiti Wida</dc:creator>
	<dc:creator>Zuraidassanaaz, Nabilah Istighfari</dc:creator>
	<dc:subject xml:lang="en-US">Biology</dc:subject>
	<dc:subject xml:lang="en-US">callus; Elephantopus scaber L.; IBA; kinetin; secondary metabolites</dc:subject>
	<dc:description xml:lang="en-US">Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol.  Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
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	<dc:date>2021-03-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/59234</dc:identifier>
	<dc:identifier>10.22146/jtbb.59234</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 6, No 1 (2021): April; jtbb59234</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/59234/30745</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/59234/13515</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/59234/13516</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/59234/14645</dc:relation>
	<dc:coverage xml:lang="en-US">Indonesia; South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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