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Antifungal Activity of Bacterial Isolates from Straw Mushroom Cultivation Medium against Phytopathogenic Fungi

Journal of Tropical Biodiversity and Biotechnology · Vol. 6 No. 1 (2021)

DOI
10.22146/jtbb.59235
ISSN
2540-9581, 2540-9573
Terbit
2021-01-20
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtrate of each bacteria. The profile of antifungal compound was identified using ethyl acetate extract followed by evaporation and gas chromatography (GC-MS) analysis. Identification of each isolates was performed using 16S rDNA amplification and sequencing. Three phytopathogenic fungi i.e Cercospora lactucae (InaCC F168), Colletotrichum gloeosporides (InaCC F304) and Fusarium oxysporum f.sp. cubense (F817) were co-cultured with bacterial isolates C2.2, C3.8, and D3.3. The C3.8 isolate has highest average inhibition activity either using isolate and filtrate. The result relatively consistent against three phytopathogenic fungi. The metabolite profile of C3.8 isolate showed the Bis(2-ethylhexyl) phthalate as the main compound with 97% similarity. Bis(2-ethylhexyl) phthalate has potential effect as antibacterial and antifungal compound. According to EzBioCloud and GeneBank databases, the C2.2 isolate identified as Bacillus tequilensis, C3.8 as Bacillus siamensis and D3.3 as Bacillus subtilis subsp. Subtilis. This study also shows the potential of Bacillus siamensis C3.8 as biocontrol against phytopathogenic fungi.

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  • Indonesian Institute of Sciences
  • University of Swabi
  • National Research and Innovation Agency
  • IPB University
  • State University of Semarang
  • Hasanuddin University
  • Walisongo State Islamic University
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Rekan penulisOrganisasiArtikel bersama
Puspita LisdiyantiIndonesian Institute of Sciences2
I Made SudianaIndonesian Institute of Sciences2
Atit KantiIndonesian Institute of Sciences2
Januarius MujiyantoState University of Semarang1
Djoko SutopoState University of Semarang1
Moh In’amiIAIN Kudus1
Wahyudin NoorInstitut Agama Islam Negeri Syaikh Abdurrahman Siddik Bangka Belitung1
Hazrat HussainUniversity of Swabi1
Tasiq AhmadUniversity of Swabi1
Said AkramUniversity of Swabi1
Shah FahadUniversity of Swabi1
Mukhtar AlamUniversity of Swabi1
Latifur RehmanUniversity of Swabi1
Dody PriadiIndonesian Institute of Sciences1
Enung Sri MulyaningsihIndonesian Institute of Sciences1
Agus ArfaniIndonesian Institute of Sciences1
Suryo WiyonoIPB University1
Wawan SetiawanIPB University1
Toga Pangihotan NapitupuluNational Research and Innovation Agency1
Idris IdrisIndonesian Institute of Sciences1
Efi Toding TondokIPB University1
I Nyoman SumertaNational Research and Innovation Agency1
Yasper Michael MambrasarIndonesian Institute of Sciences1
Indira RiastiwiIndonesian Institute of Sciences1
Yashanti Berlinda ParadisaIndonesian Institute of Sciences1
Urip PerwitasariIndonesian Institute of Sciences1
Slamet Diah VolkandariIndonesian Institute of Sciences1
Marlin Megalestin RaunsaiIndonesian Institute of Sciences1
Nurul Fitri SariIndonesian Institute of Sciences1
Leberina Kristina IboIndonesian Institute of Sciences1
Siti MeliahIndonesian Institute of Sciences1
Masaru KobayashiKyoto University1
Debora Christin PurbaniNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Antifungal Activity of Bacterial Isolates from Straw Mushroom Cultivation Medium against Phytopathogenic Fungi</dc:title>
	<dc:creator>Masrukhin, Masrukhin</dc:creator>
	<dc:creator>Putri, Ade Lia</dc:creator>
	<dc:creator>Sulistiyani, Tri Ratna</dc:creator>
	<dc:creator>Ilyas, Muhammad</dc:creator>
	<dc:creator>Purnaningsih, Ismu</dc:creator>
	<dc:creator>Saskiawan, Iwan</dc:creator>
	<dc:creator>Niam, Muhammad Yusrun</dc:creator>
	<dc:subject xml:lang="en-US">Biology; Agriculture</dc:subject>
	<dc:subject xml:lang="en-US">Antifungal; biocontrol; plant pathogen; bioactive compound; identification</dc:subject>
	<dc:description xml:lang="en-US">Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtrate of each bacteria. The profile of antifungal compound was identified using ethyl acetate extract followed by evaporation and gas chromatography (GC-MS) analysis. Identification of each isolates was performed using 16S rDNA amplification and sequencing. Three phytopathogenic fungi i.e Cercospora lactucae (InaCC F168), Colletotrichum gloeosporides (InaCC F304) and Fusarium oxysporum f.sp. cubense (F817) were co-cultured with bacterial isolates C2.2, C3.8, and D3.3. The C3.8 isolate has highest average inhibition activity either using isolate and filtrate. The result relatively consistent against three phytopathogenic fungi. The metabolite profile of C3.8 isolate showed the Bis(2-ethylhexyl) phthalate as the main compound with 97% similarity. Bis(2-ethylhexyl) phthalate has potential effect as antibacterial and antifungal compound. According to EzBioCloud and GeneBank databases, the C2.2 isolate identified as Bacillus tequilensis, C3.8 as Bacillus siamensis and D3.3 as Bacillus subtilis subsp. Subtilis. This study also shows the potential of Bacillus siamensis C3.8 as biocontrol against phytopathogenic fungi.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">DIPA Research Center for Biology, LIPI (2019)</dc:contributor>
	<dc:contributor xml:lang="en-US">Mrs. Mia Kusmiati, Mrs. Yeni Yuliani and Ms. Gita Azizah Putri</dc:contributor>
	<dc:date>2021-01-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/59235</dc:identifier>
	<dc:identifier>10.22146/jtbb.59235</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 6, No 1 (2021): April; jtbb59235</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/59235/30490</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/59235/13385</dc:relation>
	<dc:coverage xml:lang="en-US">Indonesia; South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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