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Molecular Bird Sexing of Tanimbar Cockatoos (Cacatua goffiniana) by Using Polymerase Chain Reaction Method

Journal of Tropical Biodiversity and Biotechnology · Vol. 6 No. 2 (2021)

DOI
10.22146/jtbb.59997
ISSN
2540-9581, 2540-9573
Terbit
2021-05-20
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This study aimed to determine the sex of Tanimbar Cockatoo (Cacatua goffiniana) birds by amplifying Chromodomain Helicase DNA-binding-1 (CHD-1) gene on Z and W sex chromosomes as well as to compare the quality of DNA extraction and PCR amplification products from samples derived from peripheral blood and plucked feathers. This work used five C. goffiniana birds which were collected from the Wildlife Rescue Center (WRC) in Pengasih, Kulon Progo, Yogyakarta. From each C. goffiniana, feather samples were collected by plucking feathers on the ventral wing and peripheral blood samples were taken by cutting their nails and collecting the blood into microhematocrit tubes containing heparin. The next stage was DNA extraction and DNA amplification on the CHD-1 gene using the PCR method by NP, P2, and MP primer pairs. Then, products of DNA extraction and PCR amplification were electrophoresed on 1.5% agarose gel and visualized under a UV light transilluminator with a wavelength of 260 nm. The visualization showed that samples from peripheral blood generated clearer DNA fragments compared to plucked feathers. Two of the five samples were male C. goffiniana and the other three samples were females. In the male Tanimbar Cockatoo was amplified a single DNA fragment of the Z sex chromosome in size of 300 bp, whereas in the female C. goffiniana was amplified double DNA fragments of Z and W sex chromosomes in size of 300 bp and 400 bp respectively. The DNA quality showed that the DNA quality from peripheral blood samples were better in quality than the DNA collected from plucked feather samples.

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  • Universitas Gadjah Mada
  • Tadulako University
  • Justus-Liebig-Universität Gießen
  • University of Würzburg
  • Chulalongkorn University
  • Universitas Sulawesi Barat
  • Atma Jaya Catholic University of Indonesia
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Rekan penulisOrganisasiArtikel bersama
Nastiti WijayantiUniversitas Gadjah Mada7
Medania PurwaningrumUniversitas Gadjah Mada5
Michael KannJustus-Liebig-Universität Gießen4
Hevi WihadmadyatamiUniversitas Gadjah Mada2
Krisna Noli AndrianUniversitas Gadjah Mada2
Irhamna Putri RahmawatiAfiliasi tidak diketahui2
Inggit Nindika Dianing RatriUniversitas Gadjah Mada2
Tri WibawaUniversitas Gadjah Mada1
Hera NirwatiUniversitas Gadjah Mada1
Sp.A SutaryoUniversitas Gadjah Mada1
Nenny Sri MulyaniAfiliasi tidak diketahui1
Titis Budi WidowatiAfiliasi tidak diketahui1
Purnomo HadiAfiliasi tidak diketahui1
Ida TjahajatiUniversitas Gadjah Mada1
Muh. Disna FaizalUniversitas Gadjah Mada1
Balqis Bahiya Milan NovindasariUniversitas Gadjah Mada1
Isti Ananda NurrahmiUniversitas Gadjah Mada1
Srikanth KarnatiUniversity of Würzburg1
Yohanna KayanavedaUniversitas Gadjah Mada1
Chenphop SawangmakeChulalongkorn University1
Hendro SukocoUniversitas Sulawesi Barat1
Ferbian Milas SiswantoAtma Jaya Catholic University of Indonesia1
Agung BudiyantoUniversitas Gadjah Mada1
Erif Maha Nugraha SetyawanUniversitas Gadjah Mada1
SuriansyahTadulako University1
Annisa Putri CahyaniAfiliasi tidak diketahui1
SalminTadulako University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Molecular Bird Sexing of Tanimbar Cockatoos (Cacatua goffiniana) by Using Polymerase Chain Reaction Method</dc:title>
	<dc:creator>Hidayat, Ratu Fresa Khoirotunnisa</dc:creator>
	<dc:creator>Savitri, Diana</dc:creator>
	<dc:creator>Putri, Irhamna</dc:creator>
	<dc:creator>Nugrahani, Warih Pulung</dc:creator>
	<dc:creator>Haryanto, Aris</dc:creator>
	<dc:subject xml:lang="en-US">Biochemistry; Molecular Biology</dc:subject>
	<dc:subject xml:lang="en-US">Cacatua goffiniana; CHD-1; molecular bird sexing; PCR; sex chromosome</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to determine the sex of Tanimbar Cockatoo (Cacatua goffiniana) birds by amplifying Chromodomain Helicase DNA-binding-1 (CHD-1) gene on Z and W sex chromosomes as well as to compare the quality of DNA extraction and PCR amplification products from samples derived from peripheral blood and plucked feathers. This work used five C. goffiniana birds which were collected from the Wildlife Rescue Center (WRC) in Pengasih, Kulon Progo, Yogyakarta. From each C. goffiniana, feather samples were collected by plucking feathers on the ventral wing and peripheral blood samples were taken by cutting their nails and collecting the blood into microhematocrit tubes containing heparin. The next stage was DNA extraction and DNA amplification on the CHD-1 gene using the PCR method by NP, P2, and MP primer pairs. Then, products of DNA extraction and PCR amplification were electrophoresed on 1.5% agarose gel and visualized under a UV light transilluminator with a wavelength of 260 nm. The visualization showed that samples from peripheral blood generated clearer DNA fragments compared to plucked feathers. Two of the five samples were male C. goffiniana and the other three samples were females. In the male Tanimbar Cockatoo was amplified a single DNA fragment of the Z sex chromosome in size of 300 bp, whereas in the female C. goffiniana was amplified double DNA fragments of Z and W sex chromosomes in size of 300 bp and 400 bp respectively. The DNA quality showed that the DNA quality from peripheral blood samples were better in quality than the DNA collected from plucked feather samples.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">UGM</dc:contributor>
	<dc:date>2021-05-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/59997</dc:identifier>
	<dc:identifier>10.22146/jtbb.59997</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 6, No 2 (2021): August; jtbb59997</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/59997/31294</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/59997/13519</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/59997/13521</dc:relation>
	<dc:coverage xml:lang="en-US">Indonesia; South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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