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Detection of AtRKD4 Protein During Induction of Somatic Embryogenesis in Dendrobium lineale Rolfe Transgenic Orchids Carrying 35S::GR::AtRKD4

Journal of Tropical Biodiversity and Biotechnology · Vol. 6 No. 2 (2021)

DOI
10.22146/jtbb.61783
ISSN
2540-9581, 2540-9573
Terbit
2021-06-04
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Dendrobium lineale is an Indonesian native orchid from the Spatulata section in Orchidaceae Family. This orchid is important because it is usually used as a parental plant in orchid breeding and is predicted to have a potential phytochemistry compound. In addition, in their natural habitat, this orchid is threatened due to forest exploitation and natural disaster. Therefore the precision mass propagation techniques for this orchid need to be conducted. Biotechnological approaches through inserting embryo gene such as AtRKD4 from Arabidopsis thaliana has already been successfully conducted. This study aims to check the integration stability of T-DNA harboring 35S::GR::AtRKD4 from ten selection transformants and to detect the existence of AtRKD4 protein after induction by Dexamethasone and/ Thidiazuron. The result showed that T-DNA were stably integrated into the genome of D. lineale transformants and the AtRKD4 protein with a molecular weight of 28.53 kDa was detected in D. lineale transformant plants after being induced by 15 µM DEX and 3 mgL-1 TDZ for 5 days.

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  • Universitas Gadjah Mada
  • Udayana University
  • University of Warwick
  • Universitas Pattimura
  • Diponegoro University
  • Universitas Muhammadiyah Semarang
  • Jenderal Soedirman University
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Rekan penulisOrganisasiArtikel bersama
Aziz PurwantoroUniversitas Gadjah Mada11
Sukarti MoeljopawiroUniversitas Gadjah Mada6
Windi MoseUniversitas Pattimura6
Muhammad Dylan LawrieUniversitas Gadjah Mada5
Naufal Ghozi Aditya PerdanaUniversitas Gadjah Mada5
Ari IndriantoUniversitas Gadjah Mada4
Febri Yuda KurniawanUniversitas Gadjah Mada3
Nintya SetiariDiponegoro University2
Jaka WidadaUniversitas Gadjah Mada2
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Exsyupransia MursyantiUniversitas Gadjah Mada2
Aries Bagus SasongkoUniversitas Gadjah Mada2
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1
Yan RamonaUdayana University1
I Dewa Agung Panji DwipayanaUdayana University1
Zulfa LayinaUniversitas Gadjah Mada1
Della Rosiana NingtiasUniversitas Gadjah Mada1
Falah Nur AlifiantoUniversitas Gadjah Mada1
Rina ArimarsetiowatiIndonesian Coffee and Cocoa Research Institute1
Yohana Theresia Maria AstutiInstitut Pertanian Stiper1
Ana AininaUniversitas Gadjah Mada1
Ahmad Yudis MahardhikaUniversitas Gadjah Mada1
Ni Putu Ayu Erninda Oktaviani SuputriUniversitas Gadjah Mada1
Ixora Sartika MercurianiYogyakarta State University1
Seonghoe JangAgricultural Biotechnology Research Center, Academia Sinica1
Sri Saptuti WahyuningsihLampung University1
Soenghoe JangAcademia Sinica1
Sri NopitasariUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Detection of AtRKD4 Protein During Induction of Somatic Embryogenesis in Dendrobium lineale Rolfe Transgenic Orchids Carrying 35S::GR::AtRKD4</dc:title>
	<dc:creator>Wirajagat, Gde Cahyadi</dc:creator>
	<dc:creator>Febryanti, Ni Luh Putu Kayika</dc:creator>
	<dc:creator>Puspitasari, Fitriana</dc:creator>
	<dc:creator>Sundari, Dika</dc:creator>
	<dc:creator>Gutierrez-Marcos, Jose</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US">Biology; Biotechnology; Plant Tissue Engineering</dc:subject>
	<dc:subject xml:lang="en-US">AtRKD4 gene; AtRKD4 protein; Dendrobium lineale; dexamethasone; somatic embryo; thidiazuron</dc:subject>
	<dc:description xml:lang="en-US">Dendrobium lineale is an Indonesian native orchid from the Spatulata section in Orchidaceae Family. This orchid is important because it is usually used as a parental plant in orchid breeding and is predicted to have a potential phytochemistry compound. In addition, in their natural habitat, this orchid is threatened due to forest exploitation and natural disaster. Therefore the precision mass propagation techniques for this orchid need to be conducted. Biotechnological approaches through inserting embryo gene such as AtRKD4 from Arabidopsis thaliana has already been successfully conducted. This study aims to check the integration stability of T-DNA harboring 35S::GR::AtRKD4 from ten selection transformants and to detect the existence of AtRKD4 protein after induction by Dexamethasone and/ Thidiazuron. The result showed that T-DNA were stably integrated into the genome of D. lineale transformants and the AtRKD4 protein with a molecular weight of 28.53 kDa was detected in D. lineale transformant plants after being induced by 15 µM DEX and 3 mgL-1 TDZ for 5 days.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Ministry of Research and Technology Higher Education RI on the Scheme of Rekognisi Tugas Akhir (RTA) 2020</dc:contributor>
	<dc:date>2021-06-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/61783</dc:identifier>
	<dc:identifier>10.22146/jtbb.61783</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 6, No 2 (2021): August; jtbb61783</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/61783/31438</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/61783/14297</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/61783/14365</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/61783/14514</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/61783/14727</dc:relation>
	<dc:coverage xml:lang="en-US">Indonesial; South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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