Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia

Journal of Tropical Biodiversity and Biotechnology · Vol. 6 No. 3 (2021)

DOI
10.22146/jtbb.65357
ISSN
2540-9581, 2540-9573
Terbit
2021-10-22
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F1534C) alleles in both two species. All of mosquito samples have wild type genotype of kdr alleles (V1016V and F1534F). Data were analysed using R Studio 1.4 Version by Genetics package. Results showed that the frequency of resistant alleles (G1016 and C1534) was zero, and the frequency of susceptible allele was 1 (V1016 and F1534). Insecticide bioassay could not be established due to the limited number of adult mosquitoes, so insecticide resistance status could not be determined. However, this study can be used as preliminary monitoring for the vector control program.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 123 tautan kepenulisan bersama · 49 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Airlangga
  • Universitas Negeri Surabaya
  • University of Mataram
  • Universitas Ciputra
  • Poltekkes Kemenkes Kupang
  • Universitas Wijaya Kusuma Surabaya
  • STIKES Bahrul Ulum Jombang
  • Organisasi lain
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Kismiyati KismiyatiUniversitas Airlangga5
Moch. Amin AlamsjahUniversitas Airlangga5
Agustono AgustonoUniversitas Airlangga3
Rahayu KusdarwatiUniversitas Airlangga3
Sri Pantja MadyawatiUniversitas Airlangga3
Ririh YudhastutiUniversitas Airlangga2
Wanti WantiPoltekkes Kemenkes Kupang2
Hari Basuki NotobrotoUniversitas Airlangga2
Rosmanida RosmanidaUniversitas Airlangga2
Adi PranotoUniversitas Airlangga2
Muhammad AriefUniversitas Airlangga2
Imam MustofaUniversitas Airlangga2
Tri Wahyu SuprayogiUniversitas Airlangga2
Muhammad Fajar AmrullahUniversitas Airlangga2
Ristaqul Husna BelganiaUniversitas Airlangga2
Hariyono HariyonoUniversitas Airlangga2
Leo Yosdimyati RomliSTIKES Bahrul Ulum Jombang2
Chin Xuan TanUniversiti Tunku Abdul Rahman2
Sin War NawMyitkyina University2
Mirwa Adiprahara AnggaraniUniversitas Negeri Surabaya2
Dzikra Nasyaya MahfudhahUniversitas Negeri Surabaya2
Fistara Lesti RahmafitriaUniversitas Negeri Surabaya2
Nuniek HerdyastutiUniversitas Negeri Surabaya2
Adiana Mutamsari WitaningrumUniversitas Airlangga2
Nia SaurinaUniversitas Wijaya Kusuma Surabaya1
Nur ChamidahUniversitas Airlangga1
Riries RulaningtyasUniversitas Airlangga1
Romi ErmawanUniversity of Mataram1
Yudi Her OktavionoUniversitas Airlangga1
Budi Susetyo PikirUniversitas Airlangga1
Mulyanto MulyantoUniversity of Mataram1
Widjiati WidjiatiUniversitas Airlangga1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia</dc:title>
	<dc:creator>Fauziyah, Shifa</dc:creator>
	<dc:creator>Subekti, Sri</dc:creator>
	<dc:creator>Utomo, Budi</dc:creator>
	<dc:creator>Sucipto, Teguh Hari</dc:creator>
	<dc:creator>Adrianto, Hebert</dc:creator>
	<dc:creator>Aryati, Aryati</dc:creator>
	<dc:creator>Wardhani, Puspa</dc:creator>
	<dc:creator>Soegijanto, Soegeng</dc:creator>
	<dc:subject xml:lang="en-US">Medical entomology; vector borne diseases</dc:subject>
	<dc:subject xml:lang="en-US">dengue; insecticide; kdr allele; mosquito; Surabaya; urban parks</dc:subject>
	<dc:description xml:lang="en-US">An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F1534C) alleles in both two species. All of mosquito samples have wild type genotype of kdr alleles (V1016V and F1534F). Data were analysed using R Studio 1.4 Version by Genetics package. Results showed that the frequency of resistant alleles (G1016 and C1534) was zero, and the frequency of susceptible allele was 1 (V1016 and F1534). Insecticide bioassay could not be established due to the limited number of adult mosquitoes, so insecticide resistance status could not be determined. However, this study can be used as preliminary monitoring for the vector control program.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2021-10-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/65357</dc:identifier>
	<dc:identifier>10.22146/jtbb.65357</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 6, No 3 (2021): December; jtbb65357</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/65357/32275</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/65357/16336</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/65357/16337</dc:relation>
	<dc:coverage xml:lang="en-US">Surabaya</dc:coverage>
	<dc:coverage xml:lang="en-US">This is the first study in Surabaya that was analyse the frequency of resistant allele in dengue vector collected from some urban parks.</dc:coverage>
	<dc:coverage xml:lang="en-US">Sample was collected from some urban parks in Surabaya, Indonesia. There are two species, consisting of Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus.</dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>