Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Molecular Identification of Mudskipper Fish (Periophthalmus spp.) from Baros Beach, Bantul, Yogyakarta

Journal of Tropical Biodiversity and Biotechnology · Vol. 6 No. 3 (2021)

DOI
10.22146/jtbb.66391
ISSN
2540-9581, 2540-9573
Terbit
2021-10-13
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 201 tautan kepenulisan bersama · 33 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Politeknik Bina Husada Kendari
  • Universitas Airlangga
  • University of Bengkulu
  • Kyoto University
  • Tadulako University
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Dwi Sendi PriyonoUniversitas Gadjah Mada6
Diana FebriyantiUniversitas Gadjah Mada4
Budi Setiadi DaryonoUniversitas Gadjah Mada3
Febrina Amaliya Rha’ifaUniversitas Gadjah Mada3
Amir HamidyIndonesian Institute of Sciences3
Rury EprilurahmanUniversitas Gadjah Mada2
Zuliyati RohmahUniversitas Gadjah Mada2
Lukman HakimUniversitas Gadjah Mada2
Rosichon UbaidillahIndonesian Institute of Sciences2
Tasya NuraliyahUniversitas Gadjah Mada2
Vindi DwijayantiUniversitas Gadjah Mada2
Karmilah KarmilahPoliteknik Bina Husada Kendari1
Anggun Diyan NurhasanahUniversitas Gadjah Mada1
Madyan Akmal HidayatUniversitas Gadjah Mada1
Muslifah HasanahUniversitas Gadjah Mada1
Ganies Riza AristyaUniversitas Gadjah Mada1
Adib Fakhruddin YusufUniversitas Gadjah Mada1
Adristi Shafa WidyasariUniversitas Gadjah Mada1
Niken Satuti Nur HandayaniUniversitas Gadjah Mada1
Ananto Puradi NainggolanUniversitas Gadjah Mada1
Vida Rahma LatifahUniversitas Gadjah Mada1
Vivi Indah NurcahyatiUniversitas Gadjah Mada1
Aldy Riau Wansyah HasibuanUniversitas Gadjah Mada1
Arini Dian PratiwiUniversitas Gadjah Mada1
Rindu AurantikaUniversitas Gadjah Mada1
Indra LesmanaUniversitas Gadjah Mada1
Fanni KhoirillahUniversitas Gadjah Mada1
Anjar Tri WibowoUniversitas Airlangga1
Warisatul IlmiUniversitas Gadjah Mada1
Rika Lathif HasanUniversitas Gadjah Mada1
Khadija Lung AyuUniversitas Gadjah Mada1
Nofita RatmanUniversitas Gadjah Mada1
Deiandra Jasmine AudreaUniversitas Gadjah Mada1
Vestidhia Yunisya AtmajaUniversity of Bengkulu1
Misbahul MunirKyoto University1
Tomi KasayevUniversitas Gadjah Mada1
Muhammad Zulfiqar Meizar PratamaUniversitas Gadjah Mada1
Manap TriantoTadulako University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Molecular Identification of Mudskipper Fish (Periophthalmus spp.) from Baros Beach, Bantul, Yogyakarta</dc:title>
	<dc:creator>Aji, Katon Waskito</dc:creator>
	<dc:creator>Arisuryanti, Tuty</dc:creator>
	<dc:subject xml:lang="en-US">Genetics; Fish Genetics</dc:subject>
	<dc:subject xml:lang="en-US">COI; cryptic species; DNA barcoding; mudskipper</dc:subject>
	<dc:description xml:lang="en-US">Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Laboratory of Genetics and Breeding</dc:contributor>
	<dc:date>2021-10-13</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/66391</dc:identifier>
	<dc:identifier>10.22146/jtbb.66391</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 6, No 3 (2021): December; jtbb66391</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/66391/32209</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/66391/16835</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/66391/16836</dc:relation>
	<dc:coverage xml:lang="en-US">Bantul, Yogyakarta, Indonesia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>