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Effect of Cryoprotectans and Cryopreservation on Physiological and Some Biochemical Changes of Hopea odorata Roxb. Seed

Journal of Tropical Biodiversity and Biotechnology · Vol. 7 No. 1 (2022)

DOI
10.22146/jtbb.67360
ISSN
2540-9581, 2540-9573
Terbit
2022-04-04
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Hopea odorata Roxb. is a forest plant from Dipterocarpaceae family that has important economic and ecological functions in the ecosystem. Generative propagation of H. odorata is limited because of its recalcitrant seed that cannot be stored for long periods at room temperature or even at low temperature. Cryopreservation is a seed storage technique that has the potential to prolong the shelf life of recalcitrant seeds. The aim of this study was to evaluate the effect of cryoprotectant and cryopreservation treatment on seed viability and biochemical change (electrolyte leakage, total malondialdehyde, total phenol) of H. odorata seeds. Fresh seeds of H. odorata were treated with two types of cryoprotectans namely PVS1 as non penetrating cryoprotectant and PVS2 as penetrating cryoprotectant, each type of cryoprotectant with four different concentrations (25, 50, 75 or 100%(w/v)) and four different immersion times (30, 60, 90 or 120 mins). Seeds were then stored in two different temperatures, at room temperature (28±2⁰C) or in liquid nitrogen (-196±2⁰C) for 24 hours to evaluate the cryoprotectant toxicity. The results showed that H. odorata seeds stored at room temperature and immersed either in 100%, 75% or 50% of PVS1 possess a higher viability as well as germination percentage, germination rate, vigour index and maximum growth potensial. In addition, they have lower value of electrolyte leakage, total malondialdehyde and total phenol compared to those seeds treated with PVS2. Meanwhile, both type of cryoprotectants and cryopreservation treatment in this study have not been able yet to increase seed viability of H. odorata. Cryopreservation treatments caused an increase in the total of malondialdehyde and electrolyte leakageas and these leads the inability of H. odorata seeds to germinate. PVS1 cryoprotectant seems to cause less toxic effects on H. odorata seeds but it can not prevent the negative impact of cryopreservation treatment.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Indonesian Institute of Sciences
  • Bandung Institute of Technology
  • Institut Pertanian Stiper
  • University of Palangka Raya
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Rekan penulisOrganisasiArtikel bersama
Muhammad Rifqi HaririIndonesian Institute of Sciences3
Rizmoon Nurul ZulkarnaenNational Research and Innovation Agency3
Lutfi RahmaningtiyasNational Research and Innovation Agency3
Arifin Surya Dwipa IrsyamBandung Institute of Technology2
Peniwidiyanti PeniwidiyantiNational Research and Innovation Agency2
Dian RosleineBandung Institute of Technology2
Endah SulistyawatiBandung Institute of Technology2
Ria Windi LestariUniversity of Palangka Raya2
Saripudin SaripudinNational Research and Innovation Agency2
Ponco YuliyantoNational Research and Innovation Agency2
Noviana BudiantiBandung Institute of Technology2
Yoyo SuhayaBandung Institute of Technology2
Rizkika Zakka AgustinaUniversitas Gadjah Mada1
Farida NurmalikaUniversitas Gadjah Mada1
Chlarissa Bella Asy ShyfaUniversitas Gadjah Mada1
Yohana Theresia Maria AstutiInstitut Pertanian Stiper1
Yekti Asih PurwestriUniversitas Gadjah Mada1
Lila NatasaputraAfiliasi tidak diketahui1
P. ParmiUniversitas Gadjah Mada1
Diah RachmawatiUniversitas Gadjah Mada1
R. RefliUniversitas Gadjah Mada1
Sukarti MuljopawiroUniversitas Gadjah Mada1
Widya Ayu PratiwiNational Research and Innovation Agency1
Prima Wahyu Kusuma HutabaratIndonesian Institute of Sciences1
Dwi SetyantiIndonesian Institute of Sciences1
Faozan IndresputraNational Research and Innovation Agency1
Jihan Sekar WijayantiUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of Cryoprotectans and Cryopreservation on Physiological and Some Biochemical Changes of Hopea odorata Roxb. Seed</dc:title>
	<dc:creator>Rohmah, Laila Ainur</dc:creator>
	<dc:creator>Latifah, Dian</dc:creator>
	<dc:creator>Wardani, Fitri Fatma</dc:creator>
	<dc:creator>Widjaya, Aulia Hasan</dc:creator>
	<dc:creator>Dewi, Kumala</dc:creator>
	<dc:subject xml:lang="en-US">Biology; Biochemistry; Chemical Engineering</dc:subject>
	<dc:subject xml:lang="en-US">tropical biodiversity; seed storage and biotechnology; cryopreservation, recalcitrant seed;</dc:subject>
	<dc:description xml:lang="en-US">Hopea odorata Roxb. is a forest plant from Dipterocarpaceae family that has important economic and ecological functions in the ecosystem.  Generative propagation of H. odorata is limited because of its recalcitrant seed that cannot be stored for long periods at room temperature or even at low temperature. Cryopreservation is a seed storage technique that has the potential to prolong the shelf life of recalcitrant seeds. The aim of this study was to evaluate the effect of cryoprotectant and cryopreservation treatment on seed viability and biochemical change (electrolyte leakage, total malondialdehyde, total phenol) of H. odorata seeds. Fresh seeds of H. odorata were treated with two types of cryoprotectans namely PVS1 as non penetrating cryoprotectant and PVS2 as penetrating cryoprotectant, each type of cryoprotectant with four different concentrations (25, 50, 75 or 100%(w/v)) and four different immersion times (30, 60, 90 or 120 mins). Seeds were then stored in two different temperatures, at room temperature (28±2⁰C) or in liquid nitrogen (-196±2⁰C) for 24 hours to evaluate the cryoprotectant toxicity. The results showed that H. odorata seeds stored at room temperature and immersed either in 100%, 75% or 50% of PVS1 possess a higher viability as well as germination percentage, germination rate, vigour index and maximum growth potensial. In addition, they have lower value of electrolyte leakage, total malondialdehyde and total phenol compared to those seeds treated with PVS2. Meanwhile, both type of cryoprotectants and cryopreservation treatment in this study have not been able yet to increase seed viability of H. odorata. Cryopreservation treatments caused an increase in the total of malondialdehyde and electrolyte leakageas and these leads the inability of  H. odorata seeds to germinate. PVS1 cryoprotectant seems to cause less toxic effects on H. odorata seeds but it can not prevent the negative impact of cryopreservation treatment. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Universitas Gadjah Mada Yogyakarta</dc:contributor>
	<dc:contributor xml:lang="en-US">Center for Plant Conservation Research, Indonesian Institute of Sciences (LIPI), Bogor</dc:contributor>
	<dc:date>2022-04-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/67360</dc:identifier>
	<dc:identifier>10.22146/jtbb.67360</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 7, No 1 (2022): April; jtbb67360</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/67360/33535</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/67360/17317</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/67360/17318</dc:relation>
	<dc:coverage xml:lang="en-US">South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US">Interest on Biodiversity and Vulnirable Species Issue</dc:coverage>
	<dc:coverage xml:lang="en-US">Quantitative Research</dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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