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Detection of AtRKD4 Gene and Induction of Somatic Embryo in Transformant of Phalaenopsis amabilis Carrying 35S::GR::AtRKD4

Journal of Tropical Biodiversity and Biotechnology · Vol. 8 No. 2 (2023)

DOI
10.22146/jtbb.71211
ISSN
2540-9581, 2540-9573
Terbit
2023-07-21
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Phalaenopsis amabilis (L.) Blume is a native Indonesian orchid that plays an important role in the breeding of orchid’s hybrid worldwide. The high consumer demand causes a decline in the population of orchids for commercial trade. Plant propagation through induction of somatic embryogenesis will be very beneficial, because plants can be obtained in large numbers and uniform. AtRKD4 gene is an important gene in the model plant Arabidopsis thaliana which functions very early in development stage to initiate embryo formation. The AtRKD4 gene has been inserted into the P. amabilis orchid and several transformants have been obtained. This study aims to determine the stability integration of the AtRKD4 gene in the transformant P. amabilis genome and induce somatic embryo formation on transformant orchids. Plantation of leaf explants from P. amabilis transformants on hormone-free New Phalaenopsis basic medium induced somatic embryo formation by 20%. Anatomical analysis showed that there is no difference stage between anatomy of somatic embryo development pattern in P. amabilis transformant and somatic embryo development pattern of monocot plants in general, PCR analysis using AtRKD4 specific primers showed that the embryos still contained 198 bp fragments of the AtRKD4 gene. In conclusion, the AtRKD4 gene is stably integrated in the P. amabilis orchid genome and can continuously induce the formation of somatic embryo from somatic cells of orchid transformants.

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  • Universitas Gadjah Mada
  • Nagoya University
  • Universitas Pattimura
  • University of Warwick
  • Diponegoro University
  • Universitas Muhammadiyah Semarang
  • Jenderal Soedirman University
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Rekan penulisOrganisasiArtikel bersama
Aziz PurwantoroUniversitas Gadjah Mada12
Muhammad Dylan LawrieUniversitas Gadjah Mada7
Sukarti MoeljopawiroUniversitas Gadjah Mada6
Ari IndriantoUniversitas Gadjah Mada5
Fitriana PuspitasariUniversitas Gadjah Mada4
Febri Yuda KurniawanUniversitas Gadjah Mada3
Nintya SetiariDiponegoro University2
Jaka WidadaUniversitas Gadjah Mada2
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Exsyupransia MursyantiUniversitas Gadjah Mada2
Aries Bagus SasongkoUniversitas Gadjah Mada2
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1
Zulfa LayinaUniversitas Gadjah Mada1
Della Rosiana NingtiasUniversitas Gadjah Mada1
Falah Nur AlifiantoUniversitas Gadjah Mada1
Rina ArimarsetiowatiIndonesian Coffee and Cocoa Research Institute1
Yohana Theresia Maria AstutiInstitut Pertanian Stiper1
Ana AininaUniversitas Gadjah Mada1
Ahmad Yudis MahardhikaUniversitas Gadjah Mada1
Ni Putu Ayu Erninda Oktaviani SuputriUniversitas Gadjah Mada1
Ixora Sartika MercurianiYogyakarta State University1
Seonghoe JangAgricultural Biotechnology Research Center, Academia Sinica1
Sri Saptuti WahyuningsihLampung University1
Soenghoe JangAcademia Sinica1
Sri NopitasariUniversitas Gadjah Mada1
Yuli SetiawatiIndonesian Oil Palm Research Institute1
Yasushi YoshiokaNagoya University1
Shogo MatsumotoNagoya University1
Kana NinomiyaNagoya University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Detection of AtRKD4 Gene and Induction of Somatic Embryo in Transformant of Phalaenopsis amabilis Carrying 35S::GR::AtRKD4</dc:title>
	<dc:creator>Sundari, Dika</dc:creator>
	<dc:creator>Perdana, Naufal Ghozi Aditya</dc:creator>
	<dc:creator>Mose, Windi</dc:creator>
	<dc:creator>Gutierrez -Marcos, Jose</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US">Biology</dc:subject>
	<dc:subject xml:lang="en-US">AtRKD4; embryo gene; Arabidopsis thaliana; Phalaenopsis amabilis; somatic embryo</dc:subject>
	<dc:description xml:lang="en-US">Phalaenopsis amabilis (L.) Blume is a native Indonesian orchid that plays an important role in the breeding of orchid’s hybrid worldwide. The high consumer demand causes a decline in the population of orchids for commercial trade. Plant propagation through induction of somatic embryogenesis will be very beneficial, because plants can be obtained in large numbers and uniform. AtRKD4 gene is an important gene in the model plant Arabidopsis thaliana which functions very early in development stage to initiate embryo formation. The AtRKD4 gene has been inserted into the P. amabilis orchid and several transformants have been obtained. This study aims to determine the stability integration of the AtRKD4 gene in the transformant P. amabilis genome and induce somatic embryo formation on transformant orchids. Plantation of leaf explants from P. amabilis transformants on hormone-free New Phalaenopsis basic medium induced somatic embryo formation by 20%.  Anatomical analysis showed that there is no difference stage between anatomy of somatic embryo development pattern in P. amabilis transformant and somatic embryo development pattern of monocot plants in general, PCR analysis using AtRKD4 specific primers showed that the embryos still contained 198 bp fragments of the AtRKD4 gene. In conclusion, the AtRKD4 gene is stably integrated in the P. amabilis orchid genome and can continuously induce the formation of somatic embryo from somatic cells of orchid transformants.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">The Ministry of Research and Technology RI</dc:contributor>
	<dc:contributor xml:lang="en-US">National Agency for Research and Innovation</dc:contributor>
	<dc:date>2023-07-21</dc:date>
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	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 8, No 2 (2023): August; jtbb71211</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
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	<dc:coverage xml:lang="en-US">Worlwide</dc:coverage>
	<dc:coverage xml:lang="en-US">The AtRKD4 gene has been inserted into the P. amabilis orchid and several transformants have been obtained</dc:coverage>
	<dc:coverage xml:lang="en-US">the stability integration of the AtRKD4 gene in the transformant P. amabilis genome and induce somatic embryo formation on transformant orchids.</dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
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