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Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

Journal of Tropical Biodiversity and Biotechnology · Vol. 8 No. 2 (2023)

DOI
10.22146/jtbb.78346
ISSN
2540-9581, 2540-9573
Terbit
2023-05-26
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%

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23 penulis · 50 tautan kepenulisan bersama · 23 lintas organisasi

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  • Jenderal Soedirman University
  • Indonesian Institute of Sciences
  • Universitas Gadjah Mada
  • IPB University
  • International Women University
  • Universitas Muhammadiyah Semarang
  • State University of Semarang
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Nuniek Ina RatnaningtyasJenderal Soedirman University2
Warsinah WarsinahJenderal Soedirman University1
Triyadi Hendra WijayaJenderal Soedirman University1
Jaka WidadaUniversitas Gadjah Mada1
Tri Joko RaharjoState University of Semarang1
Endang SemiartiUniversitas Gadjah Mada1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Yopi YopiIndonesian Institute of Sciences1
Feby Heryani PutriIPB University1
Andri Fadillah MartinIndonesian Institute of Sciences1
Salamiah .Afiliasi tidak diketahui1
Witiyasti ImaningsihLambung Mangkurat University1
Loekas SoesantoJenderal Soedirman University1
Hernayanti HernayantiJenderal Soedirman University1
Bambang Heru BudiantoJenderal Soedirman University1
Fajar HusenAfiliasi tidak diketahui1
Widodo WidodoIPB University1
Daniel Joko WahyonoJenderal Soedirman University1
Hendro PramonoJenderal Soedirman University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase</dc:title>
	<dc:creator>Agustiani, Rina Dwi</dc:creator>
	<dc:creator>Oedjijono, Oedjijono</dc:creator>
	<dc:creator>Rahmani, Nanik</dc:creator>
	<dc:creator>Ekowati, Nuraeni</dc:creator>
	<dc:subject xml:lang="en-US">Biology; Biochemistry</dc:subject>
	<dc:subject xml:lang="en-US">Amylolytic bacteria, Canna, Maltooligosaccharides, 16S rDNA gene</dc:subject>
	<dc:description xml:lang="en-US">The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Badan Riset dan Inovasi Nasional (BRIN) and International Woman University</dc:contributor>
	<dc:date>2023-05-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/78346</dc:identifier>
	<dc:identifier>10.22146/jtbb.78346</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 8, No 2 (2023): August; jtbb78346</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/78346/36404</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/78346/23494</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/78346/23495</dc:relation>
	<dc:coverage xml:lang="en-US">South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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