Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Induction of Synthetic Polyploids of Porang (Amorphophallus muelerri Blume) and Assessment of Its Genetic Variability Using Morphological Data and RAPD Molecular Marker

Journal of Tropical Biodiversity and Biotechnology · Vol. 8 No. 3 (2023)

DOI
10.22146/jtbb.82238
ISSN
2540-9581, 2540-9573
Terbit
2023-10-11
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation.

Kata kunci

Jaringan kolaborasi para penulis

31 penulis · 151 tautan kepenulisan bersama · 38 lintas organisasi

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Gadjah Mada
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • University of Brawijaya
  • Indonesian Institute of Sciences
  • National Research and Innovation Agency
  • State University of Malang
  • Khairun University
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Lia HapsariIndonesian Institute of Sciences2
Suyono SuyonoUniversitas Islam Negeri Maulana Malik Ibrahim2
Bagas PratesoUniversitas Islam Negeri Maulana Malik Ibrahim2
Eriyanto YusnawanNational Research and Innovation Agency2
Evika Sandi SavitriUniversitas Islam Negeri Maulana Malik Ibrahim1
Kholifah HolilUniversitas Islam Negeri Maulana Malik Ibrahim1
Ulfi Oktaviana OktavianaAfiliasi tidak diketahui1
Rasyadan Taufiq ProbojatiState University of Malang1
Sundari SundariKhairun University1
Rodiyati AzrianingsihUniversity of Brawijaya1
Isna Arofatun NikmahUniversity of Brawijaya1
Muhammad BaiquniUniversitas Gadjah Mada1
Lutfi MutaaliAfiliasi tidak diketahui1
Raden RijantaUniversitas Gadjah Mada1
Bambang Sriyanto Eko PrakosoUniversitas Gadjah Mada1
Dodi WidiyantoUniversitas Gadjah Mada1
Rizki Adriadi GhiffariUniversitas Gadjah Mada1
Agung Satriyo NugrohoUniversitas Gadjah Mada1
Estuning Tyas Wulan MeiUniversitas Gadjah Mada1
Idea Wening NuraniLaboratoire de Géographie Physique1
Joko ChristantoUniversitas Gadjah Mada1
Alia FajarwatiUniversitas Gadjah Mada1
Surani HasanatiUniversitas Gadjah Mada1
Adji SaiddinullahUniversitas Gadjah Mada1
Muhammad Galang Ramadhan Al TumusUniversitas Gadjah Mada1
Dhia Fauzia RahmanUniversitas Gadjah Mada1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Induction of Synthetic Polyploids of Porang (Amorphophallus muelerri Blume) and Assessment of Its Genetic Variability Using Morphological Data and RAPD Molecular Marker</dc:title>
	<dc:creator>Suyono, Suyono</dc:creator>
	<dc:creator>Indivia, Imey Tamara</dc:creator>
	<dc:creator>Resmisari, Ruri Siti</dc:creator>
	<dc:creator>Fitriyah, Fitriyah</dc:creator>
	<dc:creator>Wahyudi, Didik</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">genetically modified organisms;iles-iles;molecular marker;porang mutant;suweg</dc:subject>
	<dc:description xml:lang="en-US">This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Universitas Islam Negeri Maulana Malik Ibrahim Malang</dc:contributor>
	<dc:date>2023-10-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/82238</dc:identifier>
	<dc:identifier>10.22146/jtbb.82238</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 8, No 3 (2023): December; jtbb82238</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/82238/37574</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/82238/25391</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/82238/25392</dc:relation>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>