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In Vitro Seed Germination and Shoot Growth of Nepenthes jamban Chi. C. Lee, Hernawati & Akhriadi, A Unique Pitcher Plant from Indonesia

Journal of Tropical Biodiversity and Biotechnology · Vol. 9 No. 2 (2024)

DOI
10.22146/jtbb.87674
ISSN
2540-9581, 2540-9573
Terbit
2024-06-07
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done. Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia.

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  • National Research and Innovation Agency
  • Indonesian Institute of Sciences
  • Universitas Ahmad Dahlan
  • IPB University
  • Satya Wacana Christian University
  • University of Indonesia
  • Manchester Metropolitan University
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Rekan penulisOrganisasiArtikel bersama
Tri HandayaniIndonesian Institute of Sciences2
Indira RiastiwiIndonesian Institute of Sciences2
Aryani LeksonowatiIndonesian Institute of Sciences2
Yuyu Suryasari PoerbaIndonesian Institute of Sciences2
Khairul Fathan HabieUniversitas Ahmad Dahlan1
Murinto MurintoUniversitas Ahmad Dahlan1
Arfiani Nur KhusnaUniversitas Ahmad Dahlan1
Yuliawati YuliawatiSatya Wacana Christian University1
Betalini Widhi HapsariNational Research and Innovation Agency1
Deritha Ellfy RantauNational Research and Innovation Agency1
Siti NoorohmahNational Research and Innovation Agency1
Sitti Fatimah SyahidNational Research and Innovation Agency1
Dyah Retno WulandariNational Research and Innovation Agency1
Eldrian Daffa RaihanNational Research and Innovation Agency1
Aufa Rizqia HazNational Research and Innovation Agency1
Ajeng Putri KumalaNational Research and Innovation Agency1
Desriani DesrianiIndonesian Institute of Sciences1
Titiek SetyawatiAfiliasi tidak diketahui1
Sulistijorini SulistijoriniIPB University1
Andi SalamahUniversity of Indonesia1
Edi MirmantoNational Research and Innovation Agency1
Francis Q. BrearleyManchester Metropolitan University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">In Vitro Seed Germination and Shoot Growth of Nepenthes jamban Chi. C. Lee, Hernawati &amp; Akhriadi, A Unique Pitcher Plant from Indonesia</dc:title>
	<dc:creator>Prawestri, Apriliana Dyah</dc:creator>
	<dc:creator>Rahayu, Resa Sri</dc:creator>
	<dc:creator>Kurniajati, Wulan Septiningtyas</dc:creator>
	<dc:creator>Sunardi, Sunardi</dc:creator>
	<dc:creator>Mansur, Muhammad</dc:creator>
	<dc:subject xml:lang="en-US">Biology</dc:subject>
	<dc:subject xml:lang="en-US">carnivorous plant; ex situ conservation; in vitro medium; native species; seed culture</dc:subject>
	<dc:description xml:lang="en-US">The study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done.  Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Wewin Tjiasmanto, Yayasan Konservasi Biota Lahan Basah</dc:contributor>
	<dc:contributor xml:lang="en-US">Sahal Muadz</dc:contributor>
	<dc:contributor xml:lang="en-US">E-Layanan Sains, BRIN</dc:contributor>
	<dc:date>2024-06-07</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/87674</dc:identifier>
	<dc:identifier>10.22146/jtbb.87674</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 9, No 2 (2024): June; jtbb87674</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/87674/39320</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/87674/28226</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/87674/28227</dc:relation>
	<dc:coverage xml:lang="en-US">South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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