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Characterization of Flower’s Color based on CHS Gene Structure in Phalaenopsis ‘OX Queen’ and Dendrobium ‘Cheddi Jagan’ Orchids

Journal of Tropical Biodiversity and Biotechnology · Vol. 9 No. 3 (2024)

DOI
10.22146/jtbb.91511
ISSN
2540-9581, 2540-9573
Terbit
2024-09-02
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Orchids (Orchidaceae) are ornamental plants known for their high aesthetic value attributed to the shapes, colours, and fragrances of their flowers. Two types of hybrid orchids with attractive flowers, namely the Phalaenopsis 'OX Queen' orchid and the Dendrobium 'Cheddi Jagan' boast attractive flowers were used in this research, because of the beauty of its flower colour. The objective of this research is to characterise the morphology of flower colour and CHS (Chalcone Synthase) gene content that induces flower colour. The method used in this research analyzing the flower’s colour by using the RHS (Royal Horticultural Society) colour chart and molecular analysis by DNA genomic isolation and PCR amplification of gDNA for CHS gene specific primers. The results showed that purple colour is observed through the RHS, with P. 'OX Queen' coded as Deep Purple Pink (N73A) and D. 'Cheddi Jagan' coded as Strong Reddish Purple (N72C). The CHS gene can be amplified in P. ’OX Queen’ 1,287 bp and D. ’Cheddi jagan’ 3,731 bp. In both orchids, the results of amplification showed CHS motifs with conserved domains PLN03172 and PLN03170. The research results show that there is a significant difference in the morphology of the flowers of orchids. Purple colour is observed through the RHS, with P. 'OX Queen' coded as N73A and D. 'Cheddi Jagan' coded as N73C. The results showed that gDNA can be isolated by using CTAB method according to Murray and Thomson, and the CHS gene can be amplified by using CHS primers, resulting 1200 bp of P. 'OX Queen' and 2500 bp for D. 'Cheddi Jagan'. Through this study, preliminary data is expected to be obtained for future research, which is the formation of variegated flowers through editing the CRISPR/Cas9 genome in the CHS gene. This research is intended to support further studies on the formation of variegated flower patterns in P. 'OX Queen' and D. 'Cheddi Jagan’, focusing on the CHS gene using CRISPR/Cas9 technique.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Universitas Pattimura
  • University of Warwick
  • Diponegoro University
  • University of Malaya
  • Indonesian Coffee and Cocoa Research Institute
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Sukarti MoeljopawiroUniversitas Gadjah Mada11
Ari IndriantoUniversitas Gadjah Mada7
Muhammad Dylan LawrieUniversitas Gadjah Mada6
Agus Budi SetiawanUniversitas Gadjah Mada5
Windi MoseUniversitas Pattimura4
Nintya SetiariDiponegoro University4
Exsyupransia MursyantiUniversitas Gadjah Mada4
Febri Yuda KurniawanUniversitas Gadjah Mada3
Jose Gutierrez‐MarcosUniversity of Warwick3
Jaka WidadaUniversitas Gadjah Mada3
Aries Bagus SasongkoUniversitas Gadjah Mada3
Dyah Weny RespatieUniversitas Gadjah Mada2
Chee How TeoUniversity of Malaya2
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Fitriana PuspitasariUniversitas Gadjah Mada2
Naufal Ghozi Aditya PerdanaUniversitas Gadjah Mada2
Dika SundariUniversitas Gadjah Mada2
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember2
Zulfa LayinaUniversitas Gadjah Mada2
Asadi AsadiAfiliasi tidak diketahui1
Sahiral YakubAfiliasi tidak diketahui1
Ari WibowoIndonesian Coffee and Cocoa Research Institute1
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Irna AuliauzzakiaUniversitas Gadjah Mada1
Binti Nur AisahUniversitas Gadjah Mada1
Aprilia Tiara IkhwaniUniversitas Gadjah Mada1
Erlina AmbarwatiUniversitas Gadjah Mada1
Listy AnggraeniNational Research and Innovation Agency1
Chandra SetyawanUniversitas Gadjah Mada1
Aniswatul KhamidahNational Research and Innovation Agency1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Characterization of Flower’s Color based on CHS Gene Structure in Phalaenopsis ‘OX Queen’ and Dendrobium ‘Cheddi Jagan’ Orchids</dc:title>
	<dc:creator>Hanifa, Yumna Rahmadias</dc:creator>
	<dc:creator>Gildantia, Elke</dc:creator>
	<dc:creator>Kasi, Pauline Destinugrainy</dc:creator>
	<dc:creator>Purwantoro, Aziz</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US">Biology; Biotechnology</dc:subject>
	<dc:subject xml:lang="en-US">CHS gene; DNA extraction; Dendrobium ‘Cheddi Jagan’; Phalaenopsis ‘OX Queen’; Phenotypic</dc:subject>
	<dc:description xml:lang="en-US">Orchids (Orchidaceae) are ornamental plants known for their high aesthetic value attributed to the shapes, colours, and fragrances of their flowers. Two types of hybrid orchids with attractive flowers, namely the Phalaenopsis 'OX Queen' orchid and the Dendrobium 'Cheddi Jagan' boast attractive flowers were used in this research, because of the beauty of its flower colour. The objective of this research is to characterise the morphology of flower colour and CHS (Chalcone Synthase) gene content that induces flower colour. The method used in this research analyzing the flower’s colour by using the RHS (Royal Horticultural Society) colour chart and molecular analysis by DNA genomic isolation and PCR amplification of gDNA for CHS gene specific primers. The results showed that purple colour is observed through the RHS, with P. 'OX Queen' coded as Deep Purple Pink (N73A) and D. 'Cheddi Jagan' coded as Strong Reddish Purple (N72C). The CHS gene can be amplified in P. ’OX Queen’ 1,287 bp and D. ’Cheddi jagan’ 3,731 bp. In both orchids, the results of amplification showed CHS motifs with conserved domains PLN03172 and PLN03170. The research results show that there is a significant difference in the morphology of the flowers of orchids. Purple colour is observed through the RHS, with P. 'OX Queen' coded as N73A and D. 'Cheddi Jagan' coded as N73C. The results showed that gDNA can be isolated by using CTAB method according to Murray and Thomson, and the CHS gene can be amplified by using CHS primers, resulting 1200 bp of P. 'OX Queen' and 2500 bp for D. 'Cheddi Jagan'. Through this study, preliminary data is expected to be obtained for future research, which is the formation of variegated flowers through editing the CRISPR/Cas9 genome in the CHS gene. This research is intended to support further studies on the formation of variegated flower patterns in P. 'OX Queen' and D. 'Cheddi Jagan’, focusing on the CHS gene using CRISPR/Cas9 technique. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Penelitian Tesis Magister</dc:contributor>
	<dc:contributor xml:lang="en-US">Kemenristekdikti</dc:contributor>
	<dc:contributor xml:lang="en-US">Gadjah Mada University</dc:contributor>
	<dc:date>2024-09-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/91511</dc:identifier>
	<dc:identifier>10.22146/jtbb.91511</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 9, No 3 (2024): September; jtbb91511</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/91511/39802</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/91511/30373</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/91511/30374</dc:relation>
	<dc:coverage xml:lang="en-US">South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US">Orchids flower</dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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